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3.2.1.180: unsaturated chondroitin disaccharide hydrolase

This is an abbreviated version!
For detailed information about unsaturated chondroitin disaccharide hydrolase, go to the full flat file.

Word Map on EC 3.2.1.180

Reaction

beta-D-4-deoxy-DELTA4-GlcAp-(1->3)-beta-D-GalNAc6S
+
H2O
=
5-dehydro-4-deoxy-D-glucuronate
 +
N-acetyl-beta-D-galactosamine-6-O-sulfate

Synonyms

Phep_2238, Phep_2649, Phep_2830, SagUGL, SpnUGL, SpyUGL, UGL, unsaturated glucuronyl hydrolase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.180 unsaturated chondroitin disaccharide hydrolase

Engineering

Engineering on EC 3.2.1.180 - unsaturated chondroitin disaccharide hydrolase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D174N
site-directed mutagenesis
D182N
site-directed mutagenesis
F164A
site-directed mutagenesis
G362Y
site-directed mutagenesis
R57A
site-directed mutagenesis
D115N
no hydrolysis of beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S
D175N
no hydrolysis of beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S
K370A
-
site-directed mutagenesis
K370I
kcat/KM for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S is 28% of the wild-type value, kcat/Km for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc is 5fold higher than wild-type value
R236A
effectively degrades beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S. Almost inactive on beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc4S, although the substrate is degraded by the wild-type enzyme
R236H
effectively degrades beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S. Almost inactive on beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc4S, although the substrate is degraded by the wild-type enzyme
S365A
-
site-directed mutagenesis
S365G
-
site-directed mutagenesis
S365H
kcat/KM for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S is 1.6% of the wild-type value, kcat/Km for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc is similar to wild-type value
S368A
-
site-directed mutagenesis
S368G
T235A
kcat/KM for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S is 2% of the wild-type value
D115N
-
no hydrolysis of beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S
-
D175N
-
no hydrolysis of beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S
-
R236A
-
effectively degrades beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S. Almost inactive on beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc4S, although the substrate is degraded by the wild-type enzyme
-
R236H
-
effectively degrades beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S. Almost inactive on beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc4S, although the substrate is degraded by the wild-type enzyme
-