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3.2.1.18: exo-alpha-sialidase

This is an abbreviated version!
For detailed information about exo-alpha-sialidase, go to the full flat file.

Word Map on EC 3.2.1.18

Reaction

colominic acid
+
H2O
=
sialic acid
 +
lactose

Synonyms

acetylneuraminidase, Acetylneuraminyl hydrolase, acid sialidase, acylneuraminyl glycohydrolase, alpha-N-acylneuraminate glycohydrolase, alpha-neuraminidase, alpha-sialidase, alpha2,6-sialidase, alpha2,6-sialyltransferase, alpha2,6-trans-sialidase, Am0705, Am0707, Am1757, Am2085, Cytosolic sialidase, DELTA15Pd2,6ST, endo-N, endo-N-acylneuraminidase, endo-sialidase, endoNF, endosialidase, exo-alpha-sialidase, exo-sialidase, G9 sialidase, Ganglioside sialidase, ganglioside-specific sialidase, GH33C, glycosyl hydrolase, haemagglutinin-neuraminidase protein, hemagglutinin-neuraminidase, hemagglutinin-neuraminidase glycoprotein, HN, HsNEU2, IID sialidase, KDNase, Lysosomal sialidase, Major 85 kDa surface antigen, Major surface antigen, Membrane sialidase, MmNEU3, More, Mouse skeletal muscle sialidase, MSS, MTS, mucopolysaccharide N-acetylneuraminylhydrolase, Murine thymic sialidase, N-acetyl-alpha-neuraminidase, N-acetyl-alpha-neuraminidase 1, N-acetyl-alpha-neuraminidase 2, N-acetyl-alpha-neuraminidase 3, N-acetyl-alpha-neuraminidase 4, N-acetylneuraminosyl glycohydrolase, N-acylneuraminate glycohydrolase, N-acylneuraminosyl glycohydrolase, NA, NA1, NA2, NAN1, NanA, NANase, NanB, NanC, NanH, NanI, NanICD, NanJ, NanPs, Neu, NEU1, NEU2, Neu3, Neu3a, Neu3d, Neu3e, NEU4, NEU4 long, NEU4 short, NEU4L, Neu4L sialidase, neuramindase, neuraminidase, neuraminidase 1, neuraminidase 2, neuraminidase 3, neuraminidase-1, PA2794, SA85-1.1 protein, SA85-1.2 protein, SA85-1.3 protein, SiaBb1, sialidase, sialidase 4, sialidase II, sialidase Neu2, sialidase-2, sialidase-3, sialidase-4, sialidase/neuraminidase mutant F129A, sialyl hydrolase, STNA, TcTS, TDE0471, Tr6, trans-sialidase, trans-sialidase 1, TSia, VCNA

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.18 exo-alpha-sialidase

KM Value

KM Value on EC 3.2.1.18 - exo-alpha-sialidase

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.06
(trifluoromethyl)umbelliferyl alpha-sialoside
-
wild-type, hydrolysis, pH 7.6, 25°C
0.078
2'-(4-methylumbelliferyl) alpha-D-N-acetylneuraminic acid
0.028 - 1.4
2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid
1.1 - 2
2-(3-methoxyphenyl)-N-acetylneuraminic acid
0.0063 - 0.16
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid
0.6
2-O-(3-methoxyphenyl)neuraminic acid
-
-
3.3
3'-sialyllactose
-
at pH 5.0 and 37°C
0.018 - 3.3
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid
0.44
4-methylumbelliferyl alpha-NeuAc
-
37°C, pH 5.6 or 7.0
7.9
4-methylumbelliferyl alpha-sialoside
at 37°C
3.1
4-methylumbelliferyl D-N-acetylneuraminyl-alpha-(2->3)-D-galactopyranoside
-
pH 3.5, 37°C
0.06
4-methylumbelliferyl N-acetyl-alpha-D-neuraminic acid
Q5L868
at pH 6.5 and 50°C
0.01506 - 0.5652
4-methylumbelliferyl N-acetylneuraminic acid
0.04
4-methylumbelliferyl-3-deoxy-D-glycero-D-galactononulosonic acid
-
-
0.00017 - 4.9
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid
0.11 - 53
4-nitrophenyl alpha-D-N-acetylneuraminic acid
0.03 - 7.3
4-nitrophenyl alpha-sialoside
0.08 - 0.121
5-bromo-4-chloro-indolyl beta-D-galactopyranosyl-alpha-2,6-N-acetyl-neuraminic acid
4.1
5-N-acetyl-2-O-(3-methoxyphenyl)-alpha-D-neuraminic acid
-
-
2
6'-sialyllactose
-
at pH 5.0 and 37°C
0.063
alpha(2-3')-sialyllactose
recombinant enzyme, pH 6.8, 37°C
0.0049 - 5.5
alpha(2-3)-sialyllactose
0.31
alpha(2-3)sialyllactose
-
37°C, pH 5.6 or 7.0
0.28 - 0.43
alpha(2-3)sialylparagloboside
7
alpha(2-6)-sialyllactose
at 37°C
0.18
alpha-(2-3)-sialyllactose
-
pH 5.2
0.52
alpha-(2-6)-sialyllactose
-
pH 5.2
0.15
alpha-phenyl-N-acetylneuraminoside
-
-
0.5 - 3.3
alpha-sialyllactose
0.01
alpha1-Acid glycoprotein
-
-
-
0.36
bovine submaxillary mucin
-
-
-
0.29 - 1.47
colominic acid
0.0029 - 6.7
fetuin
-
0.27
Galp(beta1,2)Galf(beta1,4)GlcNAc
-
-
0.31
Galp(beta1,2)Galf(beta1,4)GlcNAcOBn
-
-
0.09
Galp(beta1,2)Galf(beta1,4)GlcNAcol
-
-
0.08
Galp(beta1,2)[Galp(beta1,3)]Gal
-
-
0.17
Galp(beta1,2)[Galp(beta1,3)]GalOBn
-
-
0.25
Galp(beta1,2)[Galp(beta1,3)]Galol
-
-
0.17
Galp(beta1,2)[Galp(beta1,3)]Galp(beta1,6)[Galf(beta1,4)]GlcNAc
-
-
0.1
Galp(beta1,2)[Galp(beta1,3)]Galp(beta1,6)[Galf(beta1,4)]GlcNAcOBn
-
-
0.82
Galp(beta1,2)[Galp(beta1,3)]Galp(beta1,6)[Galf(beta1,4)]GlcNAcol
-
-
2.35
Galp(beta1,3)Galp(beta1,6)[Galf(beta1,4)]GlcNAc
-
-
1.07
Galp(beta1,3)Galp(beta1,6)[Galf(beta1,4)]GlcNAcOBn
-
-
0.18
Galp(beta1,3)Galp(beta1,6)[Galf(beta1,4)]GlcNAcol
-
-
0.17
Galp(beta1,6)GlcNAc
-
-
0.05
Galp(beta1,6)GlcNAcOBn
-
-
0.08
Galp(beta1,6)GlcNAcol
-
-
0.15
Galp(beta1,6)[Galf(beta1,4)]GlcNAc
-
-
0.08
Galp(beta1,6)[Galf(beta1,4)]GlcNAcol
-
-
0.14 - 0.91
ganglioside GD1a
0.1 - 0.59
ganglioside GD1b
0.24 - 0.4
ganglioside GM3
0.38 - 0.62
ganglioside GT1b
0.02 - 1.75
GD1a
0.083 - 0.126
glucomacropeptide
-
0.06 - 0.18
GM1
0.04
GM2
-
-
0.09 - 0.15
GM3
0.01
GQ1
-
-
0.09
GT1
-
-
0.03
GT1b
-
-
1.6 - 7.9
N-acetylneuraminic acid-alpha-2,3-lactose
0.8
N-acetylneuraminic acid-alpha-2,6-galactose
-
-
1.2 - 5.6
N-acetylneuraminic acid-alpha-2,6-lactose
0.78 - 3
N-acetylneuraminyllactose
4.8
N-glycoloylneuraminic acid-alpha-2,3-lactose
-
-
0.17
Neu5Ac(2-3)-alpha-lactose
-
37°C, pH 6.7
0.17
Neu5Ac(alpha2,3)-lactose
-
pH 6.5, 37°C
0.261 - 0.385
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]-3,4-dimethylpyridinium
0.138 - 0.14
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]-3-methylpyridinium
0.45
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]-4-methylpyridinium
-
pH 9.5, 37°C
0.021 - 0.216
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]pyridinium
1.74
orosomucoid
-
-
-
0.000045
phenyl alpha-sialoside
pH 5.25, 37°C, mutant enzyme Y370G
0.000023
phenyl beta-sialoside
pH 5.25, 37°C, mutant enzyme Y370G
0.36 - 0.5
poly-alpha-2,8-lactose
-
0.75
sialic acid
Q5L868
at pH 6.5 and 50°C
0.1 - 4.9
sialyl-alpha-2,3-lactose
0.13 - 3.5
sialyl-alpha-2,6-lactose
0.52 - 2.38
sialyllactose
1.79 - 2.39
transferrin
-
additional information
additional information
-