adenoviral-mediated gene transfer is used to overexpress hNeu3 in the livers of male mice. hepatic Neu3 overexpression improves insulin sensitivity and glucose tolerance through modification of ganglioside composition and peroxisome proliferator-activated receptor gamma signaling
DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain Tuner (DE3)
expressed in Escherichia coli BL21(DE3) cells, the recombinant enzyme does not show any sialidase activity with the standard fluorogenic sialic-acid-based substrate
expression cloning strategy to isolate the sialidase. The clone encodes a 990-amino-acid 104 kDa open-reading-frame protein containing three domains: an N-terminal catalytic domain, a linker domain with an immunoglobulin-like fold and a C-terminal domain of unknown function. Expression in Escherichia coli indicates that the sialidase promoter is active in Escherichia coli. Overexpression in Escherichia coli resulted in several truncated forms. 54 kDa truncated variant is generated, expressed and purified
expression of His-tagged viral neuraminidase in wild-type Pichia pastoris strain GS115, an alpha-1,6-mannosyltransferase (och1)-defective Pichia pastoris mutant strain, and in Escherichia coli, resulting in a hyper-glycosylated, a low-glycosylated, and a non-glycosylated enzyme, respectively
fusion of gene encoding the neuraminidase N1 head domain residues 63-449 with the Saccharomyces cerevisiae alpha-factor secretion signal and expression in Pichia pastoris
gene nanB, DNA and amino acid sequence determination and analysis, expression in Escherichia coli of the gene rendering the recombinant cells capable of utilizing several sialoconjugates when grown on a minimal medium with these conjugates as sole carbon source
genes nanH, DNA and amino acid sequence determination and analysis, expression in Escherichia coli of the gene rendering the recombinant cells capable of utilizing several sialoconjugates when grown on a minimal medium with these conjugates as sole carbon source
isozymes Neu-1 and Neu-3, DNA sequence determination and analysis, expression of the enzymes in COS-7 cells, stably in JURKAT cells, and as His-tagged proteins in Spodoptera frugiperda Sf9 insect cells
overexpression in mouse aortic vascular smooth muscle cells. Overexpression of this gene has no effect on DNA synthesis and ERK phosphorylation in cultured vascular smooth muscle cells in the presence of TNF-alpha. The expression of the Neu3 gene leads to the inhibition of TNF-alpha induced matrix metalloproteinase-9 (MMP-9) expression in vascular smooth muscle cells. Neu3 gene expression strongly decreases MMP-9 promoter activity in response to TNF-alpha
recombinant expression of wild-type and mutant A160G, E51S, H277F, R48N, R48S, M87G, D50S, and Y370F enzymes as MBP-fusion proteins in Escherichia coli strain TB1
soluble, C-terminally His-tagged truncation mutant is functionally expressed in Spodoptera frugiperda Sf21 insect cells via baculovirus infection, protein is fused to the honeybee melittin secretion signal peptide, expression in Escherichia coli results in an inactive and insoluble enzyme
3.2.1.18 exo-alpha-sialidase
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