3.2.1.179: gellan tetrasaccharide unsaturated glucuronosyl hydrolase

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For detailed information about gellan tetrasaccharide unsaturated glucuronosyl hydrolase, go to the full flat file.

Word Map on EC 3.2.1.179

3.2.1.179

disaccharide

polysaccharide

glycosaminoglycans

lyases

chondroitin

glycoside

streptococcal

glucuronic

depolymerization

agalactiae

oligosaccharides

matrices

hyaluronate

hydrolases

phosphotransferase

xanthan

nonreducing

heteropolysaccharide

perfringens

rhamnogalacturonan

galacturonic

n-acetyl-d-galactosamine

pyogenes

vinyl

beta-d-glucosidase

Reaction

beta-D-4-deoxy-Delta4-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-D-Glcp

beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-D-Glcp

Synonyms

CaUGL, CA_C0359, More, SagUGL, UGL, unsaturated glucuronyl hydrolase

ECTree

3 Hydrolases

3.2 Glycosylases

3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

3.2.1.179 gellan tetrasaccharide unsaturated glucuronosyl hydrolase

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Results	in table
369	AA Sequence
4	Cloned(Commentary)
6	Crystallization (Commentary)
3	Expression
6	General Information
11	Inhibitors
11	kcat/KM [mM/s]
1	Ki Value [mM]
11	KM Value [mM]
2	Molecular Weight [Da]
4	Natural Substrates/ Products (Substrates)
38	Organism
6	Pathway
4	pH Optimum
12	Protein Variants
4	Purification (Commentary)
2	Reaction
14	Reference
3	Source Tissue
1	Specific Activity [micromol/min/mg]
24	Substrates and Products (Substrate)
6	Subunits
16	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
1	Temperature Range [°C]
2	Temperature Stability [°C]
11	Turnover Number [1/s]

Systematic Name

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Systematic Name on EC 3.2.1.179 - gellan tetrasaccharide unsaturated glucuronosyl hydrolase

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beta-D-4-deoxy-DELTA4-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp beta-D-4-deoxy-DELTA4-GlcAp hydrolase

The enzyme releases 4-deoxy-4(5)-unsaturated D-glucuronic acid from oligosaccharides produced by polysaccharide lyases, e.g. the tetrasaccharide beta-D-4-deoxy-Delta4-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-D-Glcp produced by EC 4.2.2.25, gellan lyase. The enzyme can also hydrolyse unsaturated chondroitin and hyaluronate disaccharides (beta-D-4-deoxy-Delta4-GlcAp-(1->3)-D-GalNAc, beta-D-4-deoxy-Delta4-GlcAp-(1->3)-D-GalNAc6S, beta-D-4-deoxy-Delta4-GlcAp2S-(1->3)-D-GalNAc, beta-D-4-deoxy-Delta4-GlcAp-(1->3)-D-GlcNAc), preferring the unsulfated disaccharides to the sulfated disaccharides.