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3.2.1.179: gellan tetrasaccharide unsaturated glucuronosyl hydrolase

This is an abbreviated version!
For detailed information about gellan tetrasaccharide unsaturated glucuronosyl hydrolase, go to the full flat file.

Word Map on EC 3.2.1.179

Reaction

beta-D-4-deoxy-Delta4-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-D-Glcp
+
H2O
=
5-dehydro-4-deoxy-D-glucuronate
 +
beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-D-Glcp

Synonyms

CaUGL, CA_C0359, More, SagUGL, UGL, unsaturated glucuronyl hydrolase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.179 gellan tetrasaccharide unsaturated glucuronosyl hydrolase

Engineering

Engineering on EC 3.2.1.179 - gellan tetrasaccharide unsaturated glucuronosyl hydrolase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D149N
almost inactive mutant enzyme, kcat/Km is significantly lower than that of the wild-type enzyme for gellan tetrasaccharide
D88N
almost inactive mutant enzyme, kcat/Km is significantly lower than that of the wild-type enzyme for gellan tetrasaccharide
G342S
kcat/KM for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S is similar to the wild-type value, kcat/Km for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc is similar to wild-type value
H339S
kcat/KM for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S is 1.2fold higher than the kcat/Km-value for the wild-type enzyme, kcat/Km for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc is 53% of the wild-type value
I344K
kcat/KM for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S is 6.1fold higher than the wild-type value, kcat/Km for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc is similar to the wild-type value
D149N
-
almost inactive mutant enzyme, kcat/Km is significantly lower than that of the wild-type enzyme for gellan tetrasaccharide
-
D88N
-
almost inactive mutant enzyme, kcat/Km is significantly lower than that of the wild-type enzyme for gellan tetrasaccharide
-
G342S
-
kcat/KM for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S is similar to the wild-type value, kcat/Km for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc is similar to wild-type value
-
H339S
-
kcat/KM for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S is 1.2fold higher than the kcat/Km-value for the wild-type enzyme, kcat/Km for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc is 53% of the wild-type value
-
I344K
-
kcat/KM for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S is 6.1fold higher than the wild-type value, kcat/Km for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc is similar to the wild-type value
-
D115N
site-directed mutagenesis, the mutant shows only negligible enzyme activity
D115N/K370S
site-directed mutagenesis, the mutant shows only negligible enzyme activity