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3.2.1.179: gellan tetrasaccharide unsaturated glucuronosyl hydrolase

This is an abbreviated version!
For detailed information about gellan tetrasaccharide unsaturated glucuronosyl hydrolase, go to the full flat file.

Word Map on EC 3.2.1.179

Reaction

beta-D-4-deoxy-Delta4-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-D-Glcp
+
H2O
=
5-dehydro-4-deoxy-D-glucuronate
 +
beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-D-Glcp

Synonyms

CaUGL, CA_C0359, More, SagUGL, UGL, unsaturated glucuronyl hydrolase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.179 gellan tetrasaccharide unsaturated glucuronosyl hydrolase

Cloned

Cloned on EC 3.2.1.179 - gellan tetrasaccharide unsaturated glucuronosyl hydrolase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
gene CA_C0359, recombinant expression of the intein-fused enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli TOP10, transcriptional analysis using quantitative PCR
gene ugl, recombinant expression of wild-type enzyme and enzyme mutants in Escherichia coli strain HMS174(DE3)pLysS