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3.2.1.174: rhamnogalacturonan rhamnohydrolase

This is an abbreviated version!
For detailed information about rhamnogalacturonan rhamnohydrolase, go to the full flat file.

Word Map on EC 3.2.1.174

Reaction

Exohydrolysis of the alpha-L-Rha-(1->4)-alpha-D-GalA bond in rhamnogalacturonan oligosaccharides with initial inversion of configuration releasing beta-L-rhamnose from the non-reducing end of rhamnogalacturonan oligosaccharides =

Synonyms

alpha-L-rhamnosidase, PcRGRH78A, RG alpha-L-rhamnopyranohydrolase, RG rhamnohydrolase, RG-rhamnohydrolase, RGI exo-hydrolase, RGI hydrolase, RGI rhamnohydrolase, RGRH

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.174 rhamnogalacturonan rhamnohydrolase

Substrates Products

Substrates Products on EC 3.2.1.174 - rhamnogalacturonan rhamnohydrolase

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
degalactosylated MHR-S + H2O
beta-L-rhamnose + ?
show the reaction diagram
-
degalactosylated saponified modified hairy regions of xylogalacturonan, from apple pectin, residue remaining after enzymatic liquefaction of pectin, galactosyl residues removed. The activity increases 78% when tested with MHR-S without galactose substitutions, and in essence the activity of this enzyme is thus hindered by galactose substitutions
-
-
?
MHR + H2O
beta-L-rhamnose + ?
show the reaction diagram
-
i.e. modified hairy regions of xylogalacturonan, from apple pectin, residue remaining after enzymatic liquefaction of pectin, the enzyme is active towards MHR-S as well as unsaponified MHR
-
-
?
MHR-S + H2O
beta-L-rhamnose + ?
show the reaction diagram
-
i.e. saponified modified hairy regions of xylogalacturonan, from apple pectin, residue remaining after enzymatic liquefaction of pectin, the enzyme is active towards MHR-S as well as unsaponified MHR
-
-
?
rhamnogalacturonan I + H2O
beta-L-rhamnose + ?
show the reaction diagram
-
RGI, degrading enzymes are active on the RGI backbone of pectin and are thus strictly specific for cleaving bonds in the repetitive [(1->2)-alpha-L-Rhap-(1->4)-alpha-D-GalpA-(1->2)] structure
-
-
?
rhamnogalacturonan I + H2O
L-rhamnose + ?
show the reaction diagram
-
Aspergillus aculeatus RGRH is active on the nonreducing end Rhap-linkage in RGI fragments
-
-
?
rhamnogalacturonan I oligosaccharides with D-galacturonic acid at the reducing end and L-rhamnopyranose at the nonreducing end + H2O
beta-L-rhamnopyranose + shortened rhamnogalacturonan I oligosaccharides with beta-D-galacturonic acid at the reducing end and at the nonreducing end
show the reaction diagram
-
alpha-L-Rha-(1->4)-[alpha-D-GalA-(1->2)-alpha-L-Rha]n-(1->4)-alpha-D-GalA. The enzyme is hindered when the terminal Rha residue is substituted at the 4-position by a beta-D-galactose. No particular preference of the enzyme for low or high molecular mass rhamnogalacturonan fragments
-
-
?
rhamnogalacturonan I oligosaccharides with L-rhamnopyranose at the nonreducing end + H2O
beta-L-rhamnopyranose + shortened rhamnogalacturonan I oligosaccharides with beta-D-galacturonic acid at the nonreducing end
show the reaction diagram
-
alpha-L-Rha-(1->4)-[alpha-D-GalA-(1->2)-alpha-L-Rha]n-(1->4)-alpha-D-GalA. The enzyme acts with inversion of configaration releasing beta-L-rhamnose from the non-reducing end of rhamnogalacturonan oligosaccharides
-
-
?
rhamnogalacturonan oligosaccharide + H2O
L-rhamnose + ?
show the reaction diagram
additional information
?
-