3.2.1.172: unsaturated rhamnogalacturonyl hydrolase
This is an abbreviated version!
For detailed information about unsaturated rhamnogalacturonyl hydrolase, go to the full flat file.
Reaction
Synonyms
CA_C0359, More, RGI hydrolase, unsaturated rhamnogalacturonyl hydrolase, URGH, URH, YesR, YteR
ECTree
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Crystallization
Crystallization on EC 3.2.1.172 - unsaturated rhamnogalacturonyl hydrolase
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hanging drop vapor diffusion method, crystal structure of YteR complexed with unsaturated chondroitin disaccharide (substrate of bacterial unsaturated glucuronyl hydrolase). The substrate is sterically hindered with the active pocket of YteR. The protruding loop of YteR prevents the UGL substrate from being bound effectively
hanging drop vapor diffusion method, structure of D143N in complex with unsaturated rhamnogalacturonan disaccharide substrate 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose is determined at 1.9 A resolution by X-ray crystallography
vapor diffusion hanging drop method, crystal structure is determined at 1.60 A by the multiwavelength anomalous dispersion method
partially purified recombinnat enzyme, hanging-drop vapor-diffusion method, mixing 0.002 ml 7.5 mg/ml protein solution with 0.002 ml reservoir solution consisting of 0.1 M Tris, pH 7.75, 16% w/v PEG 4000, 21°C, X-ray diffraction structure determination and analysis at 1.6 A resolution, modelling