3.2.1.165: exo-1,4-beta-D-glucosaminidase

This is an abbreviated version!
For detailed information about exo-1,4-beta-D-glucosaminidase, go to the full flat file.

Reaction

hydrolysis of chitosan or chitosan oligosaccharides to remove successive D-glucosamine residues from the non-reducing termini =

Synonyms

beta-GlcNase, csx, CsxA, exo-beta--D-GlcNase, exo-beta-1.4-glucosaminidase, exo-beta-D-GlcNase, exo-beta-D-glucosaminidase, exo-beta-glucosaminidase, exo-chitosanase, exochitosanase, GlcNase, GlmA, Gls93, PBPRA0520 protein

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.165 exo-1,4-beta-D-glucosaminidase

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Results	in table
6	Activating Compound
2068	AA Sequence
7	Application
1	CAS Registry Number
12	Cloned(Commentary)
4	Crystallization (Commentary)
1	Expression
1	General Stability
47	Inhibitors
20	kcat/KM [mM/s]
2	Ki Value [mM]
27	KM Value [mM]
5	Localization
4	Metals/Ions
15	Molecular Weight [Da]
9	Natural Substrates/ Products (Substrates)
48	Organism
2	Pathway
4	PDB ID
16	pH Optimum
5	pH Range
6	pH Stability
4	pI Value
4	Posttranslational Modification
44	Protein Variants
22	Purification (Commentary)
2	Reaction
3	Reaction Type
43	Reference
19	Source Tissue
13	Specific Activity [micromol/min/mg]
2	Storage Stability
169	Substrates and Products (Substrate)
23	Subunits
60	Synonyms
1	Systematic Name
18	Temperature Optimum [°C]
4	Temperature Range [°C]
9	Temperature Stability [°C]
20	Turnover Number [1/s]

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TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.087

4-nitrophenyl beta-D-glucosaminide

Photobacterium profundum

-

pH 7.0, 37°C

715133

0.19

4-nitrophenyl beta-D-glucoside

Photobacterium profundum

-

pH 7.0, 37°C

715133

0.073

alpha-D-glucosaminyl fluoride

Photobacterium profundum

Q6LUT2

mutant enzyme D139E, at pH 7.0 and 37°C

749852

68.8

cellobiose

Photobacterium profundum

-

pH 7.0, 37°C

715133

54.2

cellotetraose

Photobacterium profundum

-

pH 7.0, 37°C

715133

47.7

cellotriose

Photobacterium profundum

-

pH 7.0, 37°C

715133

0.0047 - 152.9

chitobiose

5 entries

27

chitohexaose

Amycolatopsis orientalis

-

2-amino-2-deoxy-D-glucopyranose split off, pH 5.2, 20°C

699070

28.3

chitosan

Aspergillus fumigatus

-

-

682639

23.8 - 102.6

colloidal chitosan

2 entries

-

3000

crab shell chitosan

Aspergillus fumigatus

-

at pH 5.5 and 40°C

749436

-

77.4

GlcN(beta1-4)GlcN(beta1-4)GlcN

Photobacterium profundum

-

pH 7.0, 37°C

715133

52

GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN

Photobacterium profundum

-

pH 7.0, 37°C

715133

97.6

GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN

Photobacterium profundum

-

pH 7.0, 37°C

715133

additional information

additional information

2 entries

-

0.0047

chitobiose

Photobacterium profundum

Q6LUT2

mutant enzyme D139A, at pH 7.0 and 37°C

749852

0.071

chitobiose

Photobacterium profundum

Q6LUT2

mutant enzyme D139E, at pH 7.0 and 37°C

749852

0.54

chitobiose

Photobacterium profundum

Q6LUT2

mutant enzyme D139S, at pH 7.0 and 37°C

749852

87

chitobiose

Photobacterium profundum

Q6LUT2

wild type enzyme, at pH 7.0 and 37°C

749852

152.9

chitobiose

Photobacterium profundum

-

pH 7.0, 37°C

715133

23.8

colloidal chitosan

Aspergillus oryzae

W8E1X9

wild type enzyme, at pH 5.5 and 50°C

751306

-

102.6

colloidal chitosan

Aspergillus oryzae

W8E1X9

mutant enzyme F769W, at pH 5.5 and 50°C

751306

-

additional information

additional information

Amycolatopsis orientalis

Q56F26

kcat for high-molecular mass chitosan is 2832 per min

678026

-

additional information

additional information

Amycolatopsis orientalis

-

kcat for high-molecular mass chitosan is 2832 per min

678026

-