3.2.1.165: exo-1,4-beta-D-glucosaminidase

This is an abbreviated version!
For detailed information about exo-1,4-beta-D-glucosaminidase, go to the full flat file.

Reaction

hydrolysis of chitosan or chitosan oligosaccharides to remove successive D-glucosamine residues from the non-reducing termini =

Synonyms

beta-GlcNase, csx, CsxA, exo-beta--D-GlcNase, exo-beta-1.4-glucosaminidase, exo-beta-D-GlcNase, exo-beta-D-glucosaminidase, exo-beta-glucosaminidase, exo-chitosanase, exochitosanase, GlcNase, GlmA, Gls93, PBPRA0520 protein

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.165 exo-1,4-beta-D-glucosaminidase

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Results	in table
6	Activating Compound
2068	AA Sequence
7	Application
1	CAS Registry Number
12	Cloned(Commentary)
4	Crystallization (Commentary)
1	Expression
1	General Stability
47	Inhibitors
20	kcat/KM [mM/s]
2	Ki Value [mM]
27	KM Value [mM]
5	Localization
4	Metals/Ions
15	Molecular Weight [Da]
9	Natural Substrates/ Products (Substrates)
48	Organism
2	Pathway
4	PDB ID
16	pH Optimum
5	pH Range
6	pH Stability
4	pI Value
4	Posttranslational Modification
44	Protein Variants
22	Purification (Commentary)
2	Reaction
3	Reaction Type
43	Reference
19	Source Tissue
13	Specific Activity [micromol/min/mg]
2	Storage Stability
169	Substrates and Products (Substrate)
23	Subunits
60	Synonyms
1	Systematic Name
18	Temperature Optimum [°C]
4	Temperature Range [°C]
9	Temperature Stability [°C]
20	Turnover Number [1/s]

Temperature Range

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Temperature Range on EC 3.2.1.165 - exo-1,4-beta-D-glucosaminidase

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TEMPERATURE RANGE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

30 - 60

Aspergillus flavus

-

30°C: about 70% of maximal activity, 60°C: about 45% of maximal activity

681334

30 - 70

Aspergillus fumigatus

-

30°C: about 40% of maximal activity, 70°C: about 75% of maximal activity

682639

37 - 100

Thermococcus kodakarensis

Q76HN4

37°C: about 20% of maximal activity, 100°C: about 20% of maximal activity

680443

40 - 60

Aspergillus oryzae

W8E1X9

the recombinant enzyme expressed in Escherichia coli shows more than 45% activity between 40 and 60°C. The recombinant enzyme expressed in Pichia pastoris shows more than 70% activity between 40 and 60°C

751306