3.2.1.165: exo-1,4-beta-D-glucosaminidase
This is an abbreviated version!
For detailed information about exo-1,4-beta-D-glucosaminidase, go to the full flat file.
Word Map on EC 3.2.1.165
-
3.2.1.165
-
chitosans
-
glucosamine
-
chitin
-
deacetylation
-
nonreducing
-
chitooligosaccharides
-
exo-type
-
n-acetylglucosamine
-
chitobiose
-
orientalis
-
thermococcus
-
diacetylchitobiose
-
glcnac2
-
transglycosylation
-
synthesis
-
amycolatopsis
-
chitosanolytic
-
viscosimetric
-
kodakaraensis
-
exo-splitting
-
beta-mannosidase
-
reesei
-
glcn-glcnac
-
chitosan-degrading
-
cm-sephadex
- 3.2.1.165
- chitosans
- glucosamine
- chitin
-
deacetylation
-
nonreducing
- chitooligosaccharides
-
exo-type
- n-acetylglucosamine
- chitobiose
- orientalis
- thermococcus
- diacetylchitobiose
- glcnac2
-
transglycosylation
- synthesis
-
amycolatopsis
-
chitosanolytic
-
viscosimetric
- kodakaraensis
-
exo-splitting
- beta-mannosidase
- reesei
-
glcn-glcnac
-
chitosan-degrading
-
cm-sephadex
Reaction
hydrolysis of chitosan or chitosan oligosaccharides to remove successive D-glucosamine residues from the non-reducing termini =
Synonyms
beta-GlcNase, csx, CsxA, exo-beta--D-GlcNase, exo-beta-1.4-glucosaminidase, exo-beta-D-GlcNase, exo-beta-D-glucosaminidase, exo-beta-glucosaminidase, exo-chitosanase, exochitosanase, GlcNase, GlmA, Gls93, PBPRA0520 protein
ECTree
3.2.1.165 exo-1,4-beta-D-glucosaminidaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 3.2.1.165 - exo-1,4-beta-D-glucosaminidase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
top
SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
4-methylumbelliferyl 2-amino-2-deoxy-beta-D-glucopyranoside + H2O
4-methylumbelliferone + 2-amino-2-deoxy-beta-D-glucopyranose
-
-
-
?
4-methylumbelliferyl beta-D-glucoside + H2O
4-methylumbelliferol + D-glucose
-
-
-
?
4-nitrophenyl beta-D-glucosaminide + H2O
4-nitrophenol + beta-D-glucosamine
-
-
-
-
?
beta-1,4-D-glucosamine tetrasaccharide
D-glucosamine + chitotriose
tetrasaccharide of chitosan efficiently depolymerizes chitosan to its constituent p-glucosamine monosaccharide in cooperation with endo-chitosanase
-
-
?
chitobiose + H2O
glucosamine + glucosamine
-
(GlcN)6 is sequentially cleaved into GlcN5,GlcN4, GlcN3, GlcN2, and GlcN, glucosamine is the final product
-
-
?
chitohexaitol + H2O
?
-
94% of the activity with chitopentaose
-
-
?
chitohexaose + H2O
2-amino-2-deoxy-D-glucopyranose + chitopentaose
-
GlcNn: polymerization degree of n=2-6, chitosan oligosaccharides, 0.96 nM exochiosanase, 10 mM ammonium acetate, pH 5.2, substrate concentrations: 6.25, 12.5, 25, 37.5 microM, hexa-N-acetylchitohexaose as control substrate at 25 microM
-
-
?
chitohexaose + H2O
chitopentaose + glucosamine
-
(GlcN)6 is sequentially cleaved into GlcN5,GlcN4, GlcN3, GlcN2, and GlcN, glucosamine is the final product
-
-
?
chitopentaitol + H2O
?
-
99% of the activity with chitopentaose
-
-
?
chitopentaose + H2O
chitotetraose + glucosamine
-
(GlcN)6 is sequentially cleaved into GlcN5,GlcN4, GlcN3, GlcN2, and GlcN, glucosamine is the final product
-
-
?
chitotetraose + H2O
chitotriose + glucosamine
-
(GlcN)6 is sequentially cleaved into GlcN5,GlcN4, GlcN3, GlcN2, and GlcN, glucosamine is the final product
-
-
?
chitotetraose + H2O
D-glucosamine + chitotriose
-
products at an early stage of reaction
-
-
?
chitotriitol + H2O
D-glucosamine + ?
-
85% of the activity with chitopentaitol
-
-
?
chitotriose + H2O
D-glucosamine + chitobiose
-
products at an early stage of reaction
-
-
?
colloidal chitin + H2O
D-glucosamine + ?
Penicillium sp. IB-37-2A
-
deacetylation degree 80-85%, 6.4% activity compared to soluble chitosan
-
-
?
colloidal chitosan + H2O
D-glucosamine + ?
-
56% activity compared to crab shell chitosan with more than 90% degree of acetylation
-
-
?
crab shell chitosan + H2O
D-glucosamine + ?
-
more than 90% degree of acetylation, 100% activity
-
-
?
D-glucosamine + N-acetyl-D-glucosamine
chitodimer
-
15% (w/v) ammonium sulfate is optimum for catalyzing transglycosylation reaction
-
-
?
GlcN(beta1-4)GlcN(beta1-4)GlcN + H2O
GlcN(beta1-4)GlcN + D-glucosamine
-
-
-
-
?
GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN + H2O
GlcN(beta1-4)GlcN(beta1-4)GlcN + D-glucosamine
-
-
-
-
?
GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN + H2O
GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN + D-glucosamine
-
-
-
-
?
GlcNbeta(1-4)GlcNAc + H2O
D-glucosamine + N-acetylglucosamine
-
-
-
-
?
GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcNAc + H2O
GlcNbeta(1-4)GlcNbeta(1-4)GlcNAc + D-glucosamine
p-nitrophenyl beta-D-glucosaminide + H2O
p-nitrophenol + beta-D-glucosamine
-
-
-
?
p-nitrophenyl-beta-D-glucosamine + H2O
D-glucosamine + p-nitrophenol
-
-
-
?
p-nitrophenyl-beta-D-N-acetylglucosaminide + H2O
p-nitrophenol + beta-D-N-acetylglucosamine
shrimp shell chitosan + H2O
D-glucosamine + ?
-
more than 90% degree of acetylation, 57% activity compared to crab shell chitosan with more than 90% degree of acetylation
-
-
?
soluble chitosan + H2O
D-glucosamine + ?
Penicillium sp. IB-37-2A
-
deacetylation degree 80-85%, 100% activity
-
-
?
4-nitrophenyl beta-D-glucoside + H2O
4-nitrophenol + beta-D-glucose
-
-
-
-
?
4-nitrophenyl beta-D-glucoside + H2O
4-nitrophenol + beta-D-glucose
-
-
-
-
?
alpha-D-glucosaminyl fluoride + D-glucosamine
chitobiose + fluoride
-
-
-
?
alpha-D-glucosaminyl fluoride + D-glucosamine
chitobiose + fluoride
-
-
-
?
chitobiose + H2O
D-glucosamine
-
38% of the activity with chitopentaose
-
-
?
chitoheptaose + H2O
D-glucosamine + chitohexaose
-
products at an early stage of reaction
-
-
?
chitoheptaose + H2O
D-glucosamine + chitohexaose
-
products at an early stage of reaction
-
-
?
chitohexaitol + H2O
D-glucosamine + ?
-
89% of the activity with chitopentaitol
-
-
?
chitohexaitol + H2O
D-glucosamine + ?
-
89% of the activity with chitopentaitol
-
-
?
chitohexaose + H2O
D-glucosamine
-
92% of the activity with chitopentaose
-
-
?
chitohexaose + H2O
D-glucosamine
-
chitohexaose is hydrolyzed to chitopentaose and D-glucosamine at the initial stage of the reaction. The final product is D-glucosamine
-
-
?
chitohexaose + H2O
D-glucosamine
-
chitohexaose is hydrolyzed to chitopentaose and D-glucosamine at the initial stage of the reaction. The final product is D-glucosamine
-
-
?
chitohexaose + H2O
D-glucosamine
at the early stages of the reaction, chitohexaose is converted to D-glucosamine and chitopentaose
-
-
?
chitohexaose + H2O
D-glucosamine
-
cleaved to chitopentaose and glusosamine in the initial stage of the reaction
-
-
?
chitohexaose + H2O
D-glucosamine
-
cleaved to chitopentaose and glusosamine in the initial stage of the reaction
-
-
?
chitohexaose + H2O
D-glucosamine + ?
-
chitohexaose is hydrolyzed to chitopentaose + D-glucosamine at the beginning, and then the resulting chitopentaose changes successively to a smaller chitooligosaccharide with concomitant brelease of D-glucosamine
-
-
?
chitohexaose + H2O
D-glucosamine + ?
-
chitohexaose is hydrolyzed to chitopentaose + D-glucosamine at the beginning, and then the resulting chitopentaose changes successively to a smaller chitooligosaccharide with concomitant brelease of D-glucosamine
-
-
?
chitohexaose + H2O
D-glucosamine + chitopentaose
-
chitohexaose is hydrolyzed to chitopentaose and D-glucosamine at the initial stage of the reaction. The final product is D-glucosamine
-
-
?
chitohexaose + H2O
D-glucosamine + chitopentaose
-
chitohexaose is hydrolyzed to chitopentaose and D-glucosamine at the initial stage of the reaction. The final product is D-glucosamine
-
-
?
chitopentaose + H2O
?
cleaved to chitotetraose and glucosamine in the initial stage of the reaction
-
-
?
chitopentaose + H2O
?
cleaved to chitotetraose and glucosamine in the initial stage of the reaction
-
-
?
chitopentaose + H2O
D-glucosamine
at the early stages of the reaction, chitopentaose is converted to D-glucosamine and chitotetraose
-
-
?
chitopentaose + H2O
D-glucosamine + chitotetraose
-
products at an early stage of reaction
-
-
?
chitopentaose + H2O
D-glucosamine + chitotetraose
-
products at an early stage of reaction
-
-
?
chitosan + H2O
?
-
the exo-beta-D-glucosaminidase cleaves the glycosidic link of either GlcN-beta(1,4)-GlcN or GlcN-beta(1,4)-GlcNAc
-
-
?
chitosan + H2O
?
-
the exo-beta-D-glucosaminidase cleaves the glycosidic link of either GlcN-beta(1,4)-GlcN or GlcN-beta(1,4)-GlcNAc
-
-
?
chitosan + H2O
D-glucosamine + ?
-
chitosan with more than 50% degree of acetylation, 9% activity compared to crab shell chitosan chitosan with more than 90% degree of acetylation
-
-
?
chitosan + H2O
D-glucosamine + ?
-
chitosan with more than 60% degree of acetylation, 21% activity compared to crab shell chitosan chitosan with more than 90% degree of acetylation
-
-
?
chitosan + H2O
D-glucosamine + ?
-
chitosan with more than 70% degree of acetylation, 43% activity compared to crab shell chitosan with more than 90% degree of acetylation
-
-
?
chitosan + H2O
D-glucosamine + ?
-
chitosan with more than 80% degree of acetylation, 68% activity compared to crab shell chitosan with more than 90% degree of acetylation
-
-
?
chitosan + H2O
D-glucosamine + ?
Aspergillus fumigatus IIT-004
-
chitosan with more than 50% degree of acetylation, 9% activity compared to crab shell chitosan chitosan with more than 90% degree of acetylation
-
-
?
chitosan + H2O
D-glucosamine + ?
Aspergillus fumigatus IIT-004
-
chitosan with more than 60% degree of acetylation, 21% activity compared to crab shell chitosan chitosan with more than 90% degree of acetylation
-
-
?
chitosan + H2O
D-glucosamine + ?
-
only glucosamine is produced as final product after prolonged reaction
-
-
?
chitosan + H2O
D-glucosamine + ?
-
only glucosamine is produced as final product after prolonged reaction
-
-
?
chitosan + H2O
D-glucosamine + ?
100% activity with 95% deacetylated chitosan, 88% activity with 83% deacetylated chitosan, 80% activity with 70% deacetylated chitosan, and 68% activity with 60% deacetylated chitosan
-
-
?
chitosan + H2O
D-glucosamine + ?
-
soluble chitosan, powdered chitosan and colloidal chitosan
-
-
?
chitosan + H2O
D-glucosamine + ?
-
soluble chitosan, powdered chitosan and colloidal chitosan
-
-
?
chitosan + H2O
D-glucosamine + ?
-
only D-glucosamine is produced after prolonged hydrolysis
-
-
?
chitosan + H2O
D-glucosamine + ?
-
only D-glucosamine is produced after prolonged hydrolysis
-
-
?
chitotetraitol + H2O
D-glucosamine + ?
-
97% of the activity with chitopentaitol
-
-
?
chitotetraitol + H2O
D-glucosamine + ?
-
97% of the activity with chitopentaitol
-
-
?
chitotetraose + H2O
D-glucosamine
-
99% of the activity with chitopentaose
-
-
?
chitotetraose + H2O
D-glucosamine
-
at first, the enzyme predominantly produces D-glucosamine and chitotriose, which is further degraded into D-glucosamine and chitobiose. The transglycosylation product, chitopentaose, is also produced, together with a lesser amount of chitohexaose
-
-
?
chitotetraose + H2O
D-glucosamine
at the early stages of the reaction, chitotetraose is converted to D-glucosamine and chitotriose
-
-
?
chitotriose + H2O
chitobiose + D-glucosamine
-
(GlcN)6 is sequentially cleaved into GlcN5,GlcN4, GlcN3, GlcN2, and GlcN, glucosamine is the final product
-
-
?
chitotriose + H2O
D-glucosamine
-
92% of the activity with chitopentaose
-
-
?
GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcNAc + H2O
GlcNbeta(1-4)GlcNbeta(1-4)GlcNAc + D-glucosamine
-
(GlcN)3-GlcNAc (mono-N-acetylated chitotetraose, the reducing end residue is N-acetylated) is at first degraded into (GlcN)2-GlcNAc, and then into GlcN-GlcNAc. After a longer incubation period, the substrate is finally hydrolyzed into monosaccharides, GlcN and GlcNAc
-
-
?
GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcNAc + H2O
GlcNbeta(1-4)GlcNbeta(1-4)GlcNAc + D-glucosamine
-
(GlcN)3-GlcNAc is a mono-N-acetylated chitotetraose, the reducing end residue is N-acetylated. D-glucosamine is produced from the nonreducing end together with the transglycosylation products
-
-
?
glycol chitosan + H2O
D-glucosamine + ?
-
12% activity compared to crab shell chitosan with more than 90% degree of acetylation
-
-
?
glycol chitosan + H2O
D-glucosamine + ?
Penicillium sp. IB-37-2A
-
631.3% activity compared to soluble chitosan
-
-
?
p-nitrophenyl-beta-D-N-acetylglucosaminide + H2O
p-nitrophenol + beta-D-N-acetylglucosamine
-
-
-
-
?
p-nitrophenyl-beta-D-N-acetylglucosaminide + H2O
p-nitrophenol + beta-D-N-acetylglucosamine
-
-
-
-
?
additional information
?
-
no activity is detectable against p-nitrophenyl derivatives of beta-D-glucopyranoside, beta-D-xylopyranoside, beta-D-mannoside, beta-D-glucuronide, beta-D-galactoside, beta-D-N-acetylgalactosaminide, beta-D-N-acetylglucosaminide and alpha-D-N-acetylglucosaminide
-
-
?
additional information
?
-
-
no activity is detectable against p-nitrophenyl derivatives of beta-D-glucopyranoside, beta-D-xylopyranoside, beta-D-mannoside, beta-D-glucuronide, beta-D-galactoside, beta-D-N-acetylgalactosaminide, beta-D-N-acetylglucosaminide and alpha-D-N-acetylglucosaminide
-
-
?
additional information
?
-
-
Chitosanases hydrolyze beta-1,4-glycosidic linkage of chitosan, a polysaccharide consisting mainly of D-glucosamine with a variable content of N-acetyl-D-glucosamine
-
-
?
additional information
?
-
cleaving nonreducing beta-D-glucosamine residues of oligosaccharides, highly specific due to pocket for N2 group of glucosaminidase discriminating it from glucose
-
-
?
additional information
?
-
-
cleaving nonreducing beta-D-glucosamine residues of oligosaccharides, highly specific due to pocket for N2 group of glucosaminidase discriminating it from glucose
-
-
?
additional information
?
-
-
no degradation of cellulose and starch. The exo-beta-D-glucosaminidase exhibits transglycosylation activity, resulting in the one-residue elongated oligomers
-
-
?
additional information
?
-
-
no activity with flaked chitin, colloidal chitin and cellulose
-
-
?
additional information
?
-
Aspergillus fumigatus IIT-004
-
no activity with flaked chitin, colloidal chitin and cellulose
-
-
?
additional information
?
-
-
no degradation of cellulose and starch. The exo-beta-D-glucosaminidase exhibits transglycosylation activity, resulting in the one-residue elongated oligomers
-
-
?
additional information
?
-
no hydrolysis of colloid chitin and carboxymethylcellulose
-
-
?
additional information
?
-
-
the enzyme acts on beta-1,4-linkage of chitosan from its non-reducing end and produces D-glucosamine as the exclusive end product
-
-
?
additional information
?
-
-
preparation of alpha-chitin and chitosan from shrimp processing raw byproducts
-
-
?
additional information
?
-
-
the enzyme acts on beta-1,4-linkage of chitosan from its non-reducing end and produces D-glucosamine as the exclusive end product
-
-
?
additional information
?
-
-
preparation of alpha-chitin and chitosan from shrimp processing raw byproducts
-
-
?
additional information
?
-
-
the enzyme catalyzes transglycosylation reaction on chitobiose and chitotriose
-
-
?
additional information
?
-
-
the enzyme catalyzes transglycosylation reaction on chitobiose and chitotriose
-
-
?
additional information
?
-
Penicillium sp. IB-37-2A
-
no activity with carboxymethyl cellulose, cellulose, xylan and galactomannan
-
-
?
additional information
?
-
-
the enzyme fails to release a reducing sugar from avicel and carboxylmethyl cellulose
-
-
?
additional information
?
-
-
the enzyme fails to release a reducing sugar from avicel and carboxylmethyl cellulose
-
-
?
additional information
?
-
-
the enzyme cleaves the beta-1->4-glycosidic bonds between GlcN-GlcN and GlcN-GlcNAc but not between GlcNAc-GlcN or GlcNAc-GlcNAc, cleavage of GlcN-GlcNAc, a partially acetylated chitooligomer mixture, overview. The purified enzyme displays high activity toward chitosan and (GlcN)6. No significant activity with N-carboxymethyl chitosan, or chitin, also synthetic p-nitrophenyl-beta-D-glucosaminide is not hydrolyzed. Chitosan with higher deacetylation degrees is hydrolyzed more efficiently than chitosan with lower deacetylation values. Substrate specifiicty analysis using massspectrometry. Paecilomyces exo-beta-D-GlcNase can accelerate the hydrolysis of p-nitrophenyl-beta-D-N-acetylglucosaminide, p-nitrophenyl-beta-D-N,N'-diacetylchitobioside, and p-nitrophenyl-beta-D-N-acetylgalactosaminide to release the corresponding sugar
-
-
?
additional information
?
-
-
the enzyme also catalyzes transglycosylation reactions
-
-
?
additional information
?
-
-
the enzyme also catalyzes transglycosylation reactions
-
-
?
