3.2.1.157: iota-carrageenase

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For detailed information about iota-carrageenase, go to the full flat file.

Reaction

4-O-sulfonato-beta-D-galactopyranosyl-(1-4)-3,6-anhydro-2-O-sulfonato-alpha-D-galactopyranosyl-(1-3)-4-O-sulfonato-beta-D-galactopyranosyl-(1-4)-3,6-anhydro-2-O-sulfonato-alpha-D-galactopyranosyl-(1-3)-4-O-sulfonato-beta-D-galactopyranosyl-(1-4)-3,6-anhyd =

Synonyms

AXE80_00300, Cgi, Cgi82A, CgiA, cgiA2, cgiB, CgiB_Ce, cgiF, endo-type iota-carrageenase, GH82 iota-carrageenase, iota-carrageenase, iotase, zobellia_4265

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.157 iota-carrageenase

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Results	in table
5	Activating Compound
35	AA Sequence
4	Application
1	CAS Registry Number
17	Cloned(Commentary)
4	Crystallization (Commentary)
25	General Information
1	General Stability
54	Inhibitors
3	kcat/KM [mM/s]
8	KM Value [mM]
2	Localization
50	Metals/Ions
13	Molecular Weight [Da]
40	Natural Substrates/ Products (Substrates)
47	Organism
1	Pathway
6	PDB ID
15	pH Optimum
4	pH Range
8	pH Stability
1	pI Value
8	Protein Variants
17	Purification (Commentary)
6	Reaction
36	Reference
4	Source Tissue
10	Specific Activity [micromol/min/mg]
1	Storage Stability
69	Substrates and Products (Substrate)
11	Subunits
54	Synonyms
1	Systematic Name
11	Temperature Optimum [°C]
5	Temperature Range [°C]
9	Temperature Stability [°C]
4	Turnover Number [1/s]

Temperature Stability

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Temperature Stability on EC 3.2.1.157 - iota-carrageenase

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TEMPERATURE STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

0 - 50

Flavobacterium sp. YS-80-122

A0A1W5QBK3

over 80% activity remaining at 0-30°C, 5-10% at 50°C

753830

30

Wenyingzhuangia fucanilytica

A0A1B1Y261

purified recombinant His-tagged enzyme, stable up to, 97% and 84% of the initial activity is retained after 24 h at 4°C and 25°C

754224

40

4 entries

45

Microbulbifer thermotolerans

E3W9G3

the enzyme is stable up to 45°C in 50 mM Tris/HCl, pH 7.5, containing 0.1 M NaCl and 1 mM CaCl2 for 1 h. Incubation at 45°C inactivates the enzyme completely within 5 min at either condition of low NaCl concentration (less than 10 mM) or presence of 10 mM EDTA

716089

50

2 entries

40

Cellulophaga sp.

R9UQG9

stable up to

731522

40

Cellulophaga sp.

R9UQG9

purified recombinant His-tagged enzyme, pH 6.5, 1 h, 80% activity remaining

731522

40

Cellulophaga sp.

R9UQG9

purified recombinant His-tagged enzyme, pH 6.5, 1 h, inactivation

731522

40

Microbulbifer thermotolerans

E3W9G3

purified recombinant enzyme, stable up to, inactivation at 50°C

755283

50

Cellulophaga sp.

R9UQG9

purified recombinant His-tagged enzyme, pH 6.5, 1 h, 60% activity remaining

731522

50

Cellulophaga sp. QY3

R9UQG9

purified enzyme, 1 h, over 80% activity remaining

755018