3.2.1.157: iota-carrageenase

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For detailed information about iota-carrageenase, go to the full flat file.

Reaction

4-O-sulfonato-beta-D-galactopyranosyl-(1-4)-3,6-anhydro-2-O-sulfonato-alpha-D-galactopyranosyl-(1-3)-4-O-sulfonato-beta-D-galactopyranosyl-(1-4)-3,6-anhydro-2-O-sulfonato-alpha-D-galactopyranosyl-(1-3)-4-O-sulfonato-beta-D-galactopyranosyl-(1-4)-3,6-anhyd =

Synonyms

AXE80_00300, Cgi, Cgi82A, CgiA, cgiA2, cgiB, CgiB_Ce, cgiF, endo-type iota-carrageenase, GH82 iota-carrageenase, iota-carrageenase, iotase, zobellia_4265

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.157 iota-carrageenase

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Results	in table
5	Activating Compound
35	AA Sequence
4	Application
1	CAS Registry Number
17	Cloned(Commentary)
4	Crystallization (Commentary)
25	General Information
1	General Stability
54	Inhibitors
3	kcat/KM [mM/s]
8	KM Value [mM]
2	Localization
50	Metals/Ions
13	Molecular Weight [Da]
40	Natural Substrates/ Products (Substrates)
47	Organism
1	Pathway
6	PDB ID
15	pH Optimum
4	pH Range
8	pH Stability
1	pI Value
8	Protein Variants
17	Purification (Commentary)
6	Reaction
36	Reference
4	Source Tissue
10	Specific Activity [micromol/min/mg]
1	Storage Stability
69	Substrates and Products (Substrate)
11	Subunits
54	Synonyms
1	Systematic Name
11	Temperature Optimum [°C]
5	Temperature Range [°C]
9	Temperature Stability [°C]
4	Turnover Number [1/s]

Engineering

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Engineering on EC 3.2.1.157 - iota-carrageenase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

D247A

Alteromonas fortis

-

the enzymatic activity is completely abolished

714214

E245Q

Alteromonas fortis

-

the enzymatic activity is completely abolished

714214

E310Q

Alteromonas fortis

-

the Km value is reduced by a factor of about 4, the reduction in kcat by 50fold results in a substantial decrease in the overall enzyme efficiency (about 10fold)

714214

H281A

Alteromonas fortis

-

the mutant shows 7fold decreased Km and 180fold decreased kcat value compared to the wild type enzyme with 4% relative efficiency

714214

Q222E

Alteromonas fortis

-

the enzymatic activity is abolished

714214

Q222K

Alteromonas fortis

-

the enzymatic activity is abolished

714214

D359N

2 entries

D359N

Microbulbifer thermotolerans

E3W9G3

the mutant shows reduced activity compared to the wild type enzyme. Asp359 is not critical for catalysis

716089

D359N

Microbulbifer thermotolerans JAMB-A94T

-

the mutant shows reduced activity compared to the wild type enzyme. Asp359 is not critical for catalysis

-