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3.2.1.157: iota-carrageenase

This is an abbreviated version!
For detailed information about iota-carrageenase, go to the full flat file.

Word Map on EC 3.2.1.157

Reaction

4-O-sulfonato-beta-D-galactopyranosyl-(1-4)-3,6-anhydro-2-O-sulfonato-alpha-D-galactopyranosyl-(1-3)-4-O-sulfonato-beta-D-galactopyranosyl-(1-4)-3,6-anhydro-2-O-sulfonato-alpha-D-galactopyranosyl-(1-3)-4-O-sulfonato-beta-D-galactopyranosyl-(1-4)-3,6-anhyd =

Synonyms

AXE80_00300, Cgi, Cgi82A, CgiA, cgiA2, cgiB, CgiB_Ce, cgiF, endo-type iota-carrageenase, GH82 iota-carrageenase, iota-carrageenase, iotase, zobellia_4265

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.157 iota-carrageenase

Cloned

Cloned on EC 3.2.1.157 - iota-carrageenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Bacillus subtilis
expressed in Escherichia coli BL21(DE3) cells
Alteromonas fortis
-
expressed in Escherichia coli Origami (DE3) pLysS, BL21 (DE3), C41 (DE3), and C43 (DE3) cells
Alteromonas fortis
-
gene cgi82A, recombinant expression of His6-tagged enzyme in Escherichia coli
gene cgiA, sequence comparisons and phylogenetic analysis
gene cgiA2, sequence comparisons and phylogenetic analysis
gene cgiB, sequence comparisons and phylogenetic analysis
gene cgiB_Ce, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Escherichia coli strain BL21(DE3)
gene cgiB_Ce, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene cgiF, sequence comparisons and phylogenetic analysis
gene cgiF, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
gene encoding iota-carrageenase flanked by a C-terminal hexahistidine tag and a N-terminal PelB signal peptide for targeting the gene product into Escherichia coli periplasm. The recombinant plasmid, referred to as pETIAf, is used to transform Escherichia coli BL21(DE3) strain harbouring pLysS plasmid
Alteromonas fortis
-
overexpressed in Escherichia coli BL21(DE3)
Alteromonas fortis
-
synthetic gene CGIOP, locus MF459059 in GenBank, codon-optimized gene encoding the iota-carrageenase without a signal peptide, cloned from gene cgiA of Microbulbifer thermotolerans strain JAMB-A94, construction of the integrative vector pBCGA, recombinant expression of extracellular His-tagged enzyme in Brevibacillus choshinensis strain HPD31-SP3, real-time PCR expression analysis, copy numbers of the CGIOP gene in different lines are approximately 5-6, respectively