3.2.1.155: xyloglucan-specific exo-beta-1,4-glucanase
This is an abbreviated version!
For detailed information about xyloglucan-specific exo-beta-1,4-glucanase, go to the full flat file.
Reaction
Hydrolysis of (1->4)-D-glucosidic linkages in xyloglucans so as to successively remove oligosaccharides from the chain end. =
Synonyms
Cel74A, EC 3.2.1.160, XEG74, XG, xyloglucanase, xyloglucanase Xgh74A
ECTree
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Substrates Products
Substrates Products on EC 3.2.1.155 - xyloglucan-specific exo-beta-1,4-glucanase
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REACTION DIAGRAM
4-nitrophenyl xyloglucan derivatives + H2O
oligosaccharides
artificial substrate
-
-
?
carboxymethylcellulose + H2O
carboxymethyl cellulose oligosaccharides
-
-
-
?
tamarind xyloglucan + H2O
oligosaccharides
highly specific for, exo-type reaction
with end products XXXG, XXLG/XLXG, and XLLG
-
?
xyloglucan + H2O
?
Xgh74 contributes to the in vivo degradation of the hemicellulose xyloglucan and xylan by the cellulosome of Clostridium thermocellum
-
-
?
xyloglucan + H2O
cellotetraose
tamarind xyloglucan. Xgh74A hydrolyzes every fourth beta-1,4-glucan bond in the xyloglucan backbone, thus producing decorated cellotetraose units
-
-
?
beta-glucan oligosaccharides
8% of the activity with xyloglucan
-
-
?
beta-glucan + H2O
beta-glucan oligosaccharides
-
8% of the activity with xyloglucan
-
-
?
beta-glucan + H2O
beta-glucan oligosaccharides
8% of the activity with xyloglucan
-
-
?
carboxymethyl cellulose oligosaccharides
5% of the activity with xyloglucan
-
-
?
carboxymethyl cellulose + H2O
carboxymethyl cellulose oligosaccharides
-
5% of the activity with xyloglucan
-
-
?
carboxymethyl cellulose + H2O
carboxymethyl cellulose oligosaccharides
6% of the activity with xyloglucan
-
-
?
oligosacchrides
-
highly specific for, endo-type reaction
with end products XXXG, XXLG/XLXG, and XLLG
-
?
tamarind xyloglucan + H2O
oligosacchrides
-
highly specific for, exo-type reaction
with end products XXXG, XXLG/XLXG, and XLLG
-
?
xyloglucan oligosaccharides
the enzyme is a true endo-glucanase when it acts on linear substrates without bulky substituents in the polymer backbone. When the enzyme acts on polymeric substrate with bulky side chains, its mode of action is switched to exo-like
XXXG, XXLG and XLLG oligosaccharides
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
the enzyme is a true endo-glucanase when it acts on linear substrates without bulky substituents in the polymer backbone. When the enzyme acts on polymeric substrate with bulky side chains, its mode of action is switched to exo-like
XXXG, XXLG and XLLG oligosaccharides
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
XEG74 has dual endo-mode and exo-mode activities
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
XEG74 has dual endo-mode and exo-mode activities
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
-
XXXG, XXLG and XLLG oligosaccharides
-
?
?
-
-
low activity on carboxymethyl cellulose and on glucuronoxylan. Lack of activity on amorphous cellulose
-
-
?
additional information
?
-
low activity on carboxymethyl cellulose and on glucuronoxylan. Lack of activity on amorphous cellulose
-
-
?
additional information
?
-
-
substrate specificity, carboxymethylcellulose and barley beta-glucan are poor or no substrates
-
-
?
additional information
?
-
-
substrate specificity, carboxymethylcellulose and barley beta-glucan are poor or no substrates
-
-
?
additional information
?
-
no activity towards Avicel, carboxymethylcellulose, barley beta-1,3/1,4-glucan or xylan
-
-
?
additional information
?
-
no activity towards Avicel, carboxymethylcellulose, barley beta-1,3/1,4-glucan or xylan
-
-
?
additional information
?
-
substrate specificity, carboxymethylcellulose and barley beta-glucan are poor or no substrates
-
-
?