3.2.1.154: fructan beta-(2,6)-fructosidase
This is an abbreviated version!
For detailed information about fructan beta-(2,6)-fructosidase, go to the full flat file.
Word Map on EC 3.2.1.154
Reaction
Synonyms
6-FEH, 6-FEH1, 6-FEHa, 6-fructan exohydrolase, 6-SST, AtcwINV3, beta-(2-6)-fructan exohydrolase, beta-(2-6)-linkage specific fructan-beta-fructosidase, FEH, fructan 6-exohydrolase, fructan exohydrolase, fructanexohydrolase, levanase, Lp6-FEH, Lp6-FEHa, suc:fructan 6-fructosyltransferase, sucrose:fructan 6-fructosyltransferase, Wfh-sm3
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3.2.1.154 fructan beta-(2,6)-fructosidaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 3.2.1.154 - fructan beta-(2,6)-fructosidase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
6-kestose + H2O
sucrose + D-fructose
as active as levan with native enzyme, 85% of the activity with levan with the heterologous enzyme
-
-
?
1,1-kestotetraose + H2O
?
-
5fold lower activity than with 6,6-kestotetraose
-
-
?
1,1-kestotetraose + H2O
?
-
minor hydrolase activity (16% activity compared to 6-kestotriose)
-
-
?
1-kestotriose + H2O
?
-
minor hydrolase activity (8% activity compared to 6-kestotriose)
-
-
?
levan + H2O
?
100% activity, the enzyme shows 6-FEH activity against levan (mean degree of polymerization is 20) that is 4fold higher than against 6-kestotriose(mean degree of polymerization is 3)
-
-
?
levanbiose + H2O
beta-D-fructose
-
native enzyme shows 63% of the activity with levan
-
-
?
levanbiose + H2O
beta-D-fructose
i.e. O-beta-D-fructofuranosyl-(2-6)-beta-D-fructofuranoside. 63% of the activity with levan with the native enzyme, 57% of the activity with levan with the heterologous enzyme
-
-
?
phlein + H2O
?
95% of the activity levan with native enzyme, 120% of the activity with levan with the heterologous enzyme
-
-
?
sucrose + H2O
D-fructose + D-glucose
-
3% of the activity with levan
-
-
?
sucrose + H2O
D-fructose + D-glucose
in vitro incubations with sucrose as a single substrate show a prominent formation of 6-kestotriose compared to 1-kestotriose and 6G-kestotriose (neokestose) and the formation of 1- and 6-kestotetraose from sucrose and 1-kestotriose
-
-
?
sucrose + H2O
D-fructose + D-glucose
15% activity with 100 mM sucrose and less than 1% activity with 1 mM sucrose compared to levan
-
-
?
sucrose + H2O
D-fructose + D-glucose
-
21% of the activity with levan
-
-
?
sucrose + H2O
D-fructose + D-glucose
-
9% of the activity with levan
-
-
?
sucrose + H2O
D-fructose + D-glucose
-
2% activity compared to 6-kestotriose
-
-
?
additional information
?
-
-
no cleavage of inulin, dextran, sucrsoe, raffinose and melezitose
-
-
?
additional information
?
-
-
Agave tequilana fructans are a polydisperse mixture of long degree of polymerization (DP) polymers and fructooligosaccharides (FOSs) with both b(2-1) and b(2-6) linkages. This mixture also contains highly branched agavins and graminans, and agavins are the most abundant of all. Agave tequilana fructans exhibit fluctuation of their total reducing sugar content and DP according to the plant age
-
-
?
additional information
?
-
-
hydrolysis of terminal, non-reducing 2,6-linked beta-D-fructofuranose residues in fructans
-
-
?
additional information
?
-
-
no activity with sucrose, 1,1-nystose and inulin
-
-
?
additional information
?
-
hydrolytic cleavage of terminal fructosyl residues off inulin
-
-
?
additional information
?
-
-
hydrolytic cleavage of terminal fructosyl residues off inulin
-
-
?
additional information
?
-
-
low ability to hydrolyze sucrose
-
-
?
additional information
?
-
purification of and assay with fructans from Lolium perenne, overview
-
-
?
additional information
?
-
-
purification of and assay with fructans from Lolium perenne, overview
-
-
?
additional information
?
-
the enzyme has significant activity against beta(2,1)-linked fructans, but considerably less than against beta(2,6)-linked fructans and shows weak invertase activity
-
-
?
additional information
?
-
-
less than 1% activity towards inulin and levan compared to 6-kestotriose (poor invertase activity)
-
-
?
