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3.2.1.152: mannosylglycoprotein endo-beta-mannosidase

This is an abbreviated version!
For detailed information about mannosylglycoprotein endo-beta-mannosidase, go to the full flat file.

Word Map on EC 3.2.1.152

Reaction

Man-alpha-(1-6)Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-R
+
H2O
=
Man-alpha-(1-6)Man
+
GlcNAc-beta-(1-4)GlcNAc-R

Synonyms

EBM II, endo-beta-mannosidase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.152 mannosylglycoprotein endo-beta-mannosidase

Purification

Purification on EC 3.2.1.152 - mannosylglycoprotein endo-beta-mannosidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, Poros HS column chromatography, hydroxyapatite chromatography, DEAE-Sephacel column chromatography, Octyl Sepharose CL-4B column chromatography, and Superdex 200 gel filtration
-
enzyme is purified by ammonium sulfate precipitation, DEAE-Sephacel chromatography, sephacryl S-200 gel filtration, octyl sepharose CL-4B chromatography and superdex 200 gel filtration
-
partial purification of the recombinant protein