Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.2.1.146: beta-galactofuranosidase

This is an abbreviated version!
For detailed information about beta-galactofuranosidase, go to the full flat file.

Word Map on EC 3.2.1.146

Reaction

beta-D-(1->5) galactofuranose tetramer
+
H2O
=
beta-D-(1->5) galactofuranose trimer
+
D-galactose

Synonyms

Araf-ase, beta-D-galactofuranosidase, beta-D-galactofuranosidase 1, beta-D-galactofuranosidase 2, beta-D-galactofuranosidase 3, beta-D-galactofuranosidase 4, beta-galactofuranosidase, exo beta-D-galactofuranosidase, exo-beta-D-galactofuranosidase, exo-beta-galactofuranosidase, galactofuranose-specific beta-D-galactofuranosidase, Galf-ase, Galf-specific Galf-ase, Galf-specific hydrolase, More, ORF0232, ORF0643, ORF1110, ORF2125, ORF2812

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.146 beta-galactofuranosidase

Purification

Purification on EC 3.2.1.146 - beta-galactofuranosidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity chromatography on Sepharose 4B-peptidophosphogalactomannan
-
culture filtrate of Penicillium fellutanum, DEAE-Sepharose, 4-aminophenyl 1-thio-beta-D-galactofuranoside-Sepharose
-
dialysed culture filtrate, Sepharose-Q, NeobarAQ-1, Neobar AQ-2, Superose-12, GNA-agarose
-
purified from Glucanex, a crude enzyme preparation isolated from Trichoderma harzianum
-
recombinant N-termially 2 x His6- and Nus-tagged enzyme from Escherichia coli strain BL21(DE3)CodonPlus, by nickel affinity chromatography, tag cleavage by HRV3C protease, followed by nickel affinity chromatography and ultrafiltration
recombinant N-terminally 2 x His6- and Nus-tagged enzyme from Escherichia coli strain BL21(DE3)CodonPlus, by nickel affinity chromatography, tag cleavage by HRV3C protease, followed by nickel affinity chromatography and ultrafiltration