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3.2.1.144: 3-deoxyoctulosonase

This is an abbreviated version!
For detailed information about 3-deoxyoctulosonase, go to the full flat file.

Reaction

3-deoxyoctulosonyl-lipopolysaccharide
+
H2O
=
3-deoxyoctulosonic acid
+
lipopolysaccharide

Synonyms

3-deoxy-D-manno-oct-2-ulosonic acid alpha-ketoside hydrolase, 3-deoxy-octulo-sonase, alpha-Kdo-ase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.144 3-deoxyoctulosonase

Purification

Purification on EC 3.2.1.144 - 3-deoxyoctulosonase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant extracellular enzyme from Pichia pastoris and Saccharomyces cerevisiae in large scale by ultracentrifugation of culture supernatant, followed by two steps ultrafiltration and gel filtration, recombinnat His-tagged enzyme from Pichia pastoris by nickel affinity chromatography and gel filtration
Sephacryl S200, SP-Fractogel, Con A-Sepharose, octyl-Sepharose
-