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3.2.1.143: poly(ADP-ribose) glycohydrolase

This is an abbreviated version!
For detailed information about poly(ADP-ribose) glycohydrolase, go to the full flat file.

Word Map on EC 3.2.1.143

Reaction

(ADP-ribose)n
+
H2O
=
(ADP-ribose)n-1
 +
ADP-ribose

Synonyms

ADP-ribose glycohydrolase, ADP-ribosyl-acceptor hydrolase 3, ADPRHL2, ARH3, DrPARG, FOXG_05947, Genbank AB019366-derived protein GI 6518480, Genbank U78975-derived protein GI 2062407, glycohydrolase, poly(adenosine diphosphoribose), glycohydrolase, poly(adenosine diphosphoribose) (cattle clone 4/5), glycohydrolase, poly(adenosine diphosphoribose) (Rattus norvegicus strain BUF gene Parg), PAR glycohydrolase, PAR glyco­hydrolase, PARG, PARG1, PARG110, PARG111, PARG59, poly (ADP-ribose) glycohydrolase, poly(adenosine diphosphoribose) glycohydrolase, poly(adenosine diphosphoribose) glycosidase, poly(ADP-ribose) glycohydrolase, poly(ADP-ribose) glycohydrolase (Arabidopsis thaliana gene At2g31860), poly(ADP-ribose) glycohydrolase (Arabidopsis thaliana gene At2g31870), poly(ADP-ribose) glycohydrolase (cattly clone 4/5), poly(ADP-ribose) glycohydrolase 1, poly(ADP-ribose)glycohydrolase, poly(ADP-ribosyl) glycohydrolase, poly-ADP-glycohydrolase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.143 poly(ADP-ribose) glycohydrolase

Cloned

Cloned on EC 3.2.1.143 - poly(ADP-ribose) glycohydrolase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning from genomic DNA, DNA and amino acid sequence determination and analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), quantitative RT-PCR expression analysis
expressed in Escherichia coli
expressed in Escherichia coli K12 TB1 and Rosetta ells
-
expression as glutathione S-transferase fusion protein
-
expression in Escherichia coli
expression in Escherichia coli as His-tagged protein
expression in HeLa cell
expression of His-tagged wild-type and mutant enzymes and selenomethionine-labeled enzyme in Escherichia coli strain BL21 (DE3) GOLD
expression of isoform PARG59 in HeLa cell
-
fusion to enhanced green fluorescent protein and expression in Drosophila melanogaster
-
gene ADPRHL2, recombinant expression of His-tagged human PARG catalytic domain (residues 448-976) from Escherichia coli strain Rosetta (DE3), recombinant expression of His-tagged full-length wild-type and mutant D314E enzymes in Escherichia coli strain BL21(DE3)
gene ADPRHL2, sequence comparisons, recombinant expression of N-terminally His-tagged wild-type enzyme and GST-tagged mutant enzymes in Escherichia coli strain BL21(DE3)
gene PARG, real-time PCR expression analysis
gene PARG, recombinant expression of wild-type enzyme and catalytically inactive mutant E752N, both comprising residues 385-972, in Escherichia coli strain Tuner (DE3) cells, coexpression of GroESL chaperone
gene PARG, single gene, recombinant expression of C-terminally His-tagged hPARG in Escherichia coli BL21(DE3) Gold
gene PARG1, recombinant expression of GST-tagged wild-type and mutant enzymes
phylogenetic analysis, expression of wild-type and selenomethionin-labeled enzymes in Escherichia coli strain Rosetta (DE3)
recombinant expression of His6-tagged truncated enzyme comprising residues 385-972 in Escherichia coli strain BL21 co-expressing GroESL chaperone
recombinant expression of wild-type and mutant enzymes
the catalytic domain is expressed in Escherichia coli Rosetta2 (DE3) cells as a fusion protein with a glutathione S-transferase tag at the N-terminus and a hexahistidine tag at the C-terminus