3.2.1.141: 4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase
This is an abbreviated version!
For detailed information about 4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase, go to the full flat file.
Word Map on EC 3.2.1.141
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3.2.1.141
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sulfolobus
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maltooligosaccharides
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disaccharide
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solfataricus
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starch
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mtsases
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trey
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acidocaldarius
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arthrobacter
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synthesis
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amylolytic
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alpha-1,4-glucosidic
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maltopentaose
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archaebacterium
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food industry
- 3.2.1.141
- sulfolobus
- maltooligosaccharides
- disaccharide
- solfataricus
- starch
- mtsases
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trey
- acidocaldarius
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arthrobacter
- synthesis
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amylolytic
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alpha-1,4-glucosidic
- maltopentaose
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archaebacterium
- food industry
Reaction
Synonyms
4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase, BvMTH, glycosyltrehalose trehalohydrolase, GTHase, malto-oligosyltrehalose trehalohydrolase, maltooligosyl trehalose tetrahydrolase, Maltooligosyl trehalose trehalohydrolase, maltooligosyl trehalose trehalohydrolase (Rhizobium strain M-11 clone pMBTU1 gene treZ reduced), maltooligosyltrehalose trehalohydrolase, maltooligosyltrehalose trehalosidase, MhMTH, MTH, MTHase, ST0926, ST0927, StMTHase, trehalosidase, maltooligosyltrehalose, trehalosidase, maltooligosyltrehalose (Rhizobium strain M-11 clone pBMTU1 gene treZ reduced), TreZ
ECTree
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Substrates Products
Substrates Products on EC 3.2.1.141 - 4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase
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REACTION DIAGRAM
4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O
(1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose
maltoheptaosyltrehalose + H2O
maltoheptaose + alpha,alpha'-trehalose
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-
-
-
?
(1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose
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enzyme is up-regulated under NaCl stress, starting at about 36 hours of exposure, acts together with maltooligosyltrehalose synthase
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-
r
4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O
(1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose
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enzyme is up-regulated under NaCl stress, starting at about 36 hours of exposure, acts together with maltooligosyltrehalose synthase
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-
r
4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O
(1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose
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yield of trehalose production is relatively low but may be increased in the presence of alpha-amylase
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-
?
4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O
(1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose
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hydrolysis to disaccharides after 24 h
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-
?
4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O
(1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose
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yield of trehalose production is relatively low but may be increased in the presence of alpha-amylase
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-
?
4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O
(1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose
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hydrolysis to disaccharides after 24 h
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-
?
4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O
(1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose
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hydrolysis to disaccharides after 24 h
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-
?
4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O
(1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose
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-
-
-
?
4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O
(1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose
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-
-
-
?
4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O
(1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose
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with isoamylase and maltooligosyltrehalose synthase
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-
?
maltohexaose + H2O
?
for glucose formation, maltohexaose exhibits the highest formation rate
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-
?
maltooligosaccharide + alpha,alpha'-trehalose
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-
-
?
maltooligosyltrehalose + H2O
maltooligosaccharide + alpha,alpha'-trehalose
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alpha-1,4-glucosidic and alpha-1,1-linked terminal dissaccharides of maltooligosyltrehalose
trehalose
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?
maltopentaose + alpha,alpha'-trehalose
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-
-
-
?
maltopentaosyltrehalose + H2O
maltopentaose + alpha,alpha'-trehalose
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-
-
-
?
maltopentaosyltrehalose + H2O
maltopentaose + alpha,alpha'-trehalose
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-
-
-
?
maltopentaosyltrehalose + H2O
maltopentaose + alpha,alpha'-trehalose
trehalose formation rate increases as the degree of polymerization value of the substrate increases. maltopentaosyl trehalose is the most preferred substrate for trehalose formation
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-
?
maltopentaosyltrehalose + H2O
maltopentaose + alpha,alpha'-trehalose
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-
-
-
?
trehalose + maltopentaose
93.4% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose
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-
?
maltosylpentaosyltrehalose + H2O
trehalose + maltopentaose
93.4% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose
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-
?
trehalose + maltotetraose
hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose
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-
?
maltosyltetraosyltrehalose + H2O
trehalose + maltotetraose
hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose
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-
?
maltosyltrehalose + H2O
maltose + alpha,alpha'-trehalose
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-
-
?
maltosyltrehalose + H2O
maltose + alpha,alpha'-trehalose
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-
-
?
trehalose + maltose
14.4% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose
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-
?
maltosyltrehalose + H2O
trehalose + maltose
14.4% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose
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-
?
trehalose + maltotriose
91.6% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose
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-
?
maltosyltriosyltrehalose + H2O
trehalose + maltotriose
91.6% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose
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-
?
maltotetraose + alpha,alpha'-trehalose
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?
maltotetraosyltrehalose + H2O
maltotetraose + alpha,alpha'-trehalose
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?
maltotetraosyltrehalose + H2O
maltotetraose + alpha,alpha'-trehalose
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?
maltotetraosyltrehalose + H2O
maltotetraose + alpha,alpha'-trehalose
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?
maltotetraosyltrehalose + H2O
maltotetraose + alpha,alpha'-trehalose
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?
maltotriose + alpha,alpha'-trehalose
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?
maltotriosyltrehalose + H2O
maltotriose + alpha,alpha'-trehalose
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?
maltotriosyltrehalose + H2O
maltotriose + alpha,alpha'-trehalose
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?
maltotriosyltrehalose + H2O
maltotriose + alpha,alpha'-trehalose
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sequential hydrolysis by the bifunctional fusion enzyme of maltooligosyltrehalose synthase (MhMTS) and malooligosyltrehalose trehalohydrolase (MhMTH), synthase converts alpha-1,4-glycosidic linkage into alpha-1,1 linkage to produce maltotriosyltrehalose, which is the substrate for the MTH to produce trehalose
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-
?
maltotriosyltrehalose + H2O
maltotriose + alpha,alpha'-trehalose
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?
maltotriosyltrehalose + H2O
maltotriose + alpha,alpha'-trehalose
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sequential hydrolysis by the bifunctional fusion enzyme of maltooligosyltrehalose synthase (MhMTS) and malooligosyltrehalose trehalohydrolase (MhMTH), synthase converts alpha-1,4-glycosidic linkage into alpha-1,1 linkage to produce maltotriosyltrehalose, which is the substrate for the MTH to produce trehalose
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-
?
maltotriosyltrehalose + H2O
maltotriose + alpha,alpha'-trehalose
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sequential hydrolysis by the bifunctional fusion enzyme of maltooligosyltrehalose synthase (MhMTS) and malooligosyltrehalose trehalohydrolase (MhMTH), synthase converts alpha-1,4-glycosidic linkage into alpha-1,1 linkage to produce maltotriosyltrehalose, which is the substrate for the MTH to produce trehalose
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-
?
maltotriosyltrehalose + H2O
maltotriose + alpha,alpha'-trehalose
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?
maltotriosyltrehalose + H2O
maltotriose + alpha,alpha'-trehalose
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?
maltotriosyltrehalose + H2O
maltotriose + alpha,alpha'-trehalose
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?
?
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the enzyme is involved in the biosynthesis of trehalose from maltooligosaccharides
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?
additional information
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the enzyme releases trehalose from maltooligosyltrehalose
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?
additional information
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increase in trehalose level during NaCl stress is the result of overexpression of the trehalose-forming enzymes malooligosyltrehalose synthase (EC 5.4.99.15) and maltooligosyltrehalose trehalohydrolase
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?
additional information
?
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MTHase catalyzes the release of trehalose by cleaving the alpha-1,4-glucosidic linkage next to the alpha-1,1-linked terminal disaccharide of maltooligosyltrehalose, D255, E286, and D380 are essential catalytic residues, while residues A259, Y328, F355, and R356 are related to enzyme selectivity, mutational analysis, overview
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?
additional information
?
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hydrolysis of G3T-G5T and G5-G7. Production of trehalose from starch together with isoamylase and maltooligosyltrehalose synthase, overview
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?
additional information
?
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the crystal structure reveals a scheme for substrate recognition that involves two unique interactions: stacking of Tyr325 with the terminal glucose ring of the trehalose moiety and perpendicularly placement of Trp215 to the pyranose rings at the subsites -1 and +1 glucose
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?
additional information
?
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substrate potato starch in combined reaction with trehalose-producing (1->4)-alpha-D-glucan 1-alpha-D-glucosylmutase, MTSase (EC 5.4.99.15), recombinant enzyme
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?
additional information
?
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Saccharolobus solfataricus ATCC 33909
substrate potato starch in combined reaction with trehalose-producing (1->4)-alpha-D-glucan 1-alpha-D-glucosylmutase, MTSase (EC 5.4.99.15), recombinant enzyme
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-
?
additional information
?
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the crystal structure reveals a scheme for substrate recognition that involves two unique interactions: stacking of Tyr325 with the terminal glucose ring of the trehalose moiety and perpendicularly placement of Trp215 to the pyranose rings at the subsites -1 and +1 glucose
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-
?
additional information
?
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MTHase catalyzes the release of trehalose by cleaving the alpha-1,4-glucosidic linkage next to the alpha-1,1-linked terminal disaccharide of maltooligosyltrehalose, D255, E286, and D380 are essential catalytic residues, while residues A259, Y328, F355, and R356 are related to enzyme selectivity, mutational analysis, overview
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-
?
additional information
?
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hydrolysis of G3T-G5T and G5-G7. Production of trehalose from starch together with isoamylase and maltooligosyltrehalose synthase, overview
-
-
?
additional information
?
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maltooligosyl trehalose trehalohydrolase (MTHase) catalyzes the release of trehalose by splitting the alpha-1,4-glucosidic linkage adjacent to the alpha-1,1-glucosidic linkage of maltooligosyl trehalose
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-
?
additional information
?
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maltooligosyl trehalose trehalohydrolase (MTHase) catalyzes the release of trehalose by splitting the alpha-1,4-glucosidic linkage adjacent to the alpha-1,1-glucosidic linkage of maltooligosyl trehalose
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-
?
additional information
?
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maltooligosyl trehalose trehalohydrolase (MTHase) catalyzes the release of trehalose by splitting the alpha-1,4-glucosidic linkage adjacent to the alpha-1,1-glucosidic linkage of maltooligosyl trehalose. The recombinant MTHase can catalyze the production of trehalose from starch at high yield when coupled with the maltooligosyl trehalose synthase from the same strain
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?
additional information
?
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maltooligosyl trehalose trehalohydrolase (MTHase) catalyzes the release of trehalose by splitting the alpha-1,4-glucosidic linkage adjacent to the alpha-1,1-glucosidic linkage of maltooligosyl trehalose. The recombinant MTHase can catalyze the production of trehalose from starch at high yield when coupled with the maltooligosyl trehalose synthase from the same strain
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?
additional information
?
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maltooligosyl trehalose trehalohydrolase (MTHase) catalyzes the release of trehalose by splitting the alpha-1,4-glucosidic linkage adjacent to the alpha-1,1-glucosidic linkage of maltooligosyl trehalose
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?
additional information
?
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maltooligosyl trehalose trehalohydrolase (MTHase) catalyzes the release of trehalose by splitting the alpha-1,4-glucosidic linkage adjacent to the alpha-1,1-glucosidic linkage of maltooligosyl trehalose. The recombinant MTHase can catalyze the production of trehalose from starch at high yield when coupled with the maltooligosyl trehalose synthase from the same strain
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?
additional information
?
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the recombinant mutant enzyme is active against starch in combination with enzyme MTSase, EC 5.4.99.15, that produces trehalose from starch
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?
additional information
?
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the recombinant mutant enzyme is active against starch in combination with enzyme MTSase, EC 5.4.99.15, that produces trehalose from starch
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?
additional information
?
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the recombinant mutant enzyme is active against starch in combination with enzyme MTSase, EC 5.4.99.15, that produces trehalose from starch
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?
additional information
?
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the recombinant mutant enzyme is active against starch in combination with enzyme MTSase, EC 5.4.99.15, that produces trehalose from starch
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-
?
additional information
?
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the recombinant mutant enzyme is active against starch in combination with enzyme MTSase, EC 5.4.99.15, that produces trehalose from starch
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-
?
additional information
?
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the recombinant mutant enzyme is active against starch in combination with enzyme MTSase, EC 5.4.99.15, that produces trehalose from starch
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-
?
additional information
?
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the recombinant mutant enzyme is active against starch in combination with enzyme MTSase, EC 5.4.99.15, that produces trehalose from starch
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-
?
additional information
?
-
the recombinant mutant enzyme is active against starch in combination with enzyme MTSase, EC 5.4.99.15, that produces trehalose from starch
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-
?
additional information
?
-
the recombinant mutant enzyme is active against starch in combination with enzyme MTSase, EC 5.4.99.15, that produces trehalose from starch
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-
?
additional information
?
-
the recombinant mutant enzyme is active against starch in combination with enzyme MTSase, EC 5.4.99.15, that produces trehalose from starch
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-
?