3.2.1.141: 4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase
This is an abbreviated version!
For detailed information about 4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase, go to the full flat file.
Word Map on EC 3.2.1.141
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3.2.1.141
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sulfolobus
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maltooligosaccharides
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disaccharide
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solfataricus
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starch
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mtsases
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trey
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acidocaldarius
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arthrobacter
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synthesis
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amylolytic
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alpha-1,4-glucosidic
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maltopentaose
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archaebacterium
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food industry
- 3.2.1.141
- sulfolobus
- maltooligosaccharides
- disaccharide
- solfataricus
- starch
- mtsases
-
trey
- acidocaldarius
-
arthrobacter
- synthesis
-
amylolytic
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alpha-1,4-glucosidic
- maltopentaose
-
archaebacterium
- food industry
Reaction
Synonyms
4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase, BvMTH, glycosyltrehalose trehalohydrolase, GTHase, malto-oligosyltrehalose trehalohydrolase, maltooligosyl trehalose tetrahydrolase, Maltooligosyl trehalose trehalohydrolase, maltooligosyl trehalose trehalohydrolase (Rhizobium strain M-11 clone pMBTU1 gene treZ reduced), maltooligosyltrehalose trehalohydrolase, maltooligosyltrehalose trehalosidase, MhMTH, MTH, MTHase, ST0926, ST0927, StMTHase, trehalosidase, maltooligosyltrehalose, trehalosidase, maltooligosyltrehalose (Rhizobium strain M-11 clone pBMTU1 gene treZ reduced), TreZ
ECTree
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Specific Activity
Specific Activity on EC 3.2.1.141 - 4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase
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0.1
0.74
1.23
472
492
5.31
52
597
787
814
additional information
0.1
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D380A MTHase, hydrolysis of maltotetraosyltrehalose at 60°C in 50 mM citrate phosphate buffer, pH 5
0.74
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E286A MTHase, hydrolysis of maltotetraosyltrehalose at 60°C in 50 mM citrate phosphate buffer, pH 5
1.23
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D255A MTHase, hydrolysis of maltotetraosyltrehalose at 60°C in 50 mM citrate phosphate buffer, pH 5
472
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R356K MTHase, hydrolysis of maltotetraosyltrehalose at 60°C in 50 mM citrate phosphate buffer, pH 5
492
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Y328F MTHase, hydrolysis of maltotetraosyltrehalose at 60°C in 50 mM citrate phosphate buffer, pH 5
5.31
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W218A MTHase, hydrolysis of maltotetraosyltrehalose at 60°C in 50 mM citrate phosphate buffer, pH 5
52
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W218F MTHase, hydrolysis of maltotetraosyltrehalose at 60°C in 50 mM citrate phosphate buffer, pH 5
597
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F355Y MTHase, hydrolysis of maltotetraosyltrehalose at 60°C in 50 mM citrate phosphate buffer, pH 5
787
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A259S MTHase, hydrolysis of maltotetraosyltrehalose at 60°C in 50 mM citrate phosphate buffer, pH 5
814
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wild-type MTHase, hydrolysis of maltotetraosyltrehalose at 60°C in 50 mM citrate phosphate buffer, pH 5
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trehalose formation (combined action of MTHase and maltooligosyltrehalose synthase) upon NaCl exposure for 36 h: 0.069 mg/h/ml, 48 h: 0.117 mg/h/ml, 54 h: 0.094 mg/h/ml
additional information
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5 mM maltooligosaccharides, 100mM sodium phosphate buffer, pH 5, 70°C, 30 min, with substrate soluble starch 24 h