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3.2.1.141: 4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase

This is an abbreviated version!
For detailed information about 4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase, go to the full flat file.

Word Map on EC 3.2.1.141

Reaction

maltotetraosyl trehalose
+
H2O
=
maltotetraose
 +
trehalose

Synonyms

4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase, BvMTH, glycosyltrehalose trehalohydrolase, GTHase, malto-oligosyltrehalose trehalohydrolase, maltooligosyl trehalose tetrahydrolase, Maltooligosyl trehalose trehalohydrolase, maltooligosyl trehalose trehalohydrolase (Rhizobium strain M-11 clone pMBTU1 gene treZ reduced), maltooligosyltrehalose trehalohydrolase, maltooligosyltrehalose trehalosidase, MhMTH, MTH, MTHase, ST0926, ST0927, StMTHase, trehalosidase, maltooligosyltrehalose, trehalosidase, maltooligosyltrehalose (Rhizobium strain M-11 clone pBMTU1 gene treZ reduced), TreZ

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.141 4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase

Cloned

Cloned on EC 3.2.1.141 - 4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA construct pRBMhMTH expressed in Escherichia coli BL21(DE3)pLysS
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Escherichia coli BL21(DE3)pLysS, LB medium, vector pRSET-B
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Escherichia coli Rosetta (DE3) with mutated vector pET-15b-deltaH-treZ, Terrific broth, 37°C
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expressed in Escherichia coli BL21 (DE3) pLysS cells
expression in Escherichia coli
expression of wild-type and mutant enzymes in Escherichia coli
gene ST0926, DNA and amino acid sequence determination and analysis, sequence comparisons, a specific deletion mutant is expressed as His6-tagged protein in Escherichia coli as soluble, active acidic and thermophilic enzyme. ORFs ST0926/ST0927 in the Archaeon Sulfurisphaera tokodaii strain 7 cannot be expressed in vivo, indicating they might exist as nonfunctional pseudogenes
gene ST0927, DNA and amino acid sequence determination and analysis, sequence comparisons, a specific deletion mutant is expressed as His6-tagged protein in Escherichia coli as soluble, active acidic and thermophilic enzyme. ORFs ST0926/ST0927 in the Archaeon Sulfurisphaera tokodaii strain 7 cannot be expressed in vivo, indicating they might exist as nonfunctional pseudogenes
gene treZ, codon-optimized synthetic gene, recombinant expression of SUMO-fused enzyme in Escherichi coli strains BL21 (DE3) and Origami (DE3), subcloning in Escherichia coli strain JM109, host strain and induction strategy optimization for high-efficiency expression, method reaching a maximum of 204.6 U/ml for the soluble enzyme, detailed overview
gene treZ, expression in fusion with maltooligosyltrehalose synthase in Oryzsa sativa subsp. japonica cv. Nakdong using Agrobacterium tumefaciens LBA4404 transfection method
-
gene treZ, recombinant enzyme expression in Brevibacillus brevis from pET-24a(+)-treZ-fused to pSVN resulting in 170 /g wet cell
overexpression of the treZ gene and the treY/treZ synthetic operon
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pET-15b-deltaH-treZ vector expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL
the artifical gene is cloned into yeast expression vector pPICZalphaA, and then overexpressed in Pichia pastoris. The yeast strains possess a highly efficient and stably expression for enzyme production. The enzyme activity level from the secretion of recombinant protein reaches 800 U/mg protein
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