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3.2.1.139: alpha-glucuronidase

This is an abbreviated version!
For detailed information about alpha-glucuronidase, go to the full flat file.

Word Map on EC 3.2.1.139

Reaction

an alpha-D-glucuronoside
+
H2O
=
an alcohol
 +
D-glucuronate

Synonyms

(4-O-methyl)-alpha-glucuronidase, 4-O-methylglucuronidase, aGlu, Agu115, Agu115A, Agu4B, AguA, alpha-(4-O-methyl)-D-glucuronidase, alpha-D-glucuronidase, Alpha-glucosiduronase, alpha-glucosiduronate glucuronohydrolase, alpha-glucuronidase, amylouronate hydrolase-I, Aryl alpha-glucuronidase, AugA, AUH-I, BoAgu115A, DEG75-AG, GH115, GH115 glucuronidase, GH67, GH67 alpha-glucuronidase, GlcA115A, GlcA67A, GLRI, glucuronidase, alpha-, glycosyl hydrolase family 115 alpha-glucuronidase, non-xylanolytic alpha-glucuronidase, p-nitrophenyl alpha-D-glucuronide-hydrolyzing enzyme, Pjdr2_5977, PNP-GAase, RUM630-AG, Sde_1755, TrDCase, TreDCase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.139 alpha-glucuronidase

Purification

Purification on EC 3.2.1.139 - alpha-glucuronidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 enzym forms: CM-I and CM-II
-
HiTrap column chromatography
-
Ni-NTA agarose column chromatography, DEAE-Sepharose column chromatography, and Superose 12 gel filtration
Ni-Sepharose column chromatography
-
partially purified by gel filtration
-
recombinant
recombinant enzyme
-
recombinant enzyme from Saccharomyces cerevisiae strain MH1000pbk by ultrafiltration
-
recombinant His-tagged enzyme from Pichia pastoris by nickel affinity chromatography
-
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) to homogeneity by immobilized metal ion affinity chromatography and gel filtration
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3)pLysE by immobilized metal affinity chromatography and gell filtration
-
soluble phase onto HisTrap HP column, eluted with imidazole gradient in sodium phosphate buffer, pH 8, and 300 mM sodium chloride
supernatant concentrated on Amicon 10 kDa cut-off membranes, anion-exchange chromatography on HiTrap DEAE-FF column with NaCl gradient in 50 mM sodium-phosphate buffer, pH 7.0, fractions between 0.2 and 0.25 M NaCl pooled, concentrated, desalted, equilibrated in 50 mM acetate buffer, pH 4.0, with 2 M (NH4)2SO4, subjected to hydrophobic interaction chromatography on Butyl-FF column, eluted with (NH4)2SO4 gradient, active fractions between 1.1 and 0.61 M (NH4)2SO4 pooled, desalted, concentrated, subjected to 2 additional anion-exchange chromatography steps on Tricorn MonoQ 5/50GL column, first equilibrated with 50 mM sodium acetate buffer, pH 4.0, eluted with NaCl gradient, then equilibrated with 50 mM sodium phosphate buffer, pH 7.0 and NaCl gradient, concentration with Microcon 10 kDa cut-off membrane filter
Superose 12 gel filtration
Toyopearl SuperQ column chromatography, Toyopearl phenyl column chromatography, Resource Q column chromatography, and TSKgel G3000SWXL gel filtration
-