3.2.1.137: mannan exo-1,2-1,6-alpha-mannosidase

This is an abbreviated version!
For detailed information about mannan exo-1,2-1,6-alpha-mannosidase, go to the full flat file.

Reaction

Synonyms

alpha-1,2/3/6-mannosidase, JBM, mannosidase, exo-1,2-1,6-alpha

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.137 mannan exo-1,2-1,6-alpha-mannosidase

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Results	in table
1	CAS Registry Number
5	Inhibitors
3	KM Value [mM]
1	Molecular Weight [Da]
1	Natural Substrates/ Products (Substrates)
2	Organism
2	Pathway
1	pH Optimum
1	pH Range
1	pH Stability
1	pI Value
1	Purification (Commentary)
1	Reaction
1	Reaction Type
2	Reference
1	Source Tissue
1	Specific Activity [micromol/min/mg]
1	Storage Stability
8	Substrates and Products (Substrate)
3	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
2	Temperature Stability [°C]

Substrates Products

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Substrates Products on EC 3.2.1.137 - mannan exo-1,2-1,6-alpha-mannosidase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

D-mannan

D-mannose

Cellulomonas sp.

-

hydrolysis of 1,2-alpha-D-, and 1,6-alpha-D-linkages in yeast mannan, releasing D-mannose, hydrolysis rate of alpha-1,3-linkage is very low

646830

-

646830

?

D-mannan + H2O

D-mannose

Cellulomonas sp.

-

hydrolysis of 1,2-alpha-D-, and 1,6-alpha-D-linkages in yeast mannan, releasing D-mannose

646830

-

-

?

glycoprotein A + H2O

deglycosylated glycoprotein A + alpha-D-mannose

Canavalia ensiformis

-

-

752578

-

-

?

glycoprotein B + H2O

deglycosylated glycoprotein B + alpha-D-mannose

Canavalia ensiformis

-

-

752578

-

-

?

granulocyte colony-stimulating factor protein + H2O

deglycosylated granulocyte colony-stimulating factor protein + alpha-D-mannose

Canavalia ensiformis

-

recombinant human rhGCSF expressed in a GFI2.0 glycoengineered Pichia pastoris strain and possessing O-linked glycosylation composed of a single O-linked mannose residue

752578

-

-

?

granulocyte/macrophage colony-stimulating factor + H2O

deglycosylated granulocyte/macrophage colony-stimulating factor + alpha-D-mannose

Canavalia ensiformis

-

i.e. GM-CSF, sargramostim, recombinantly expressed in Pichia pastoris

752578

-

-

?

additional information

?

-

2 entries

additional information

?

-

Canavalia ensiformis

-

the alpha-1,2/3/6-mannosidase from Jack bean is able to hydrolyze the Man-O-Ser/Thr glycosidic linkage on four individual undenatured intact glycoproteins. Jack bean alpha-1,2/3/6-mannosidase removal of O-linked mannose is not specific for intact glycoproteins produced in Pichia pastoris

752578

-

-

?

additional information

?

-

Cellulomonas sp.

-

only slight activity towards 4-nitrophenyl-alpha-D-mannoside and no hydrolysis of O-alpha-D-mannopyranosyl-1,2-D-mannitol

646830

-

-

?