3.2.1.136: glucuronoarabinoxylan endo-1,4-beta-xylanase

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For detailed information about glucuronoarabinoxylan endo-1,4-beta-xylanase, go to the full flat file.

Reaction

Synonyms

1,4-beta-D-xylan xylanohydrolase, Agu115A, beta-1,4-endoxylanase, bifunctional cellulase/xylanase, endo-beta-xylanase, endoarabinoxylanase, endoglucanase, endoxylanase, feraxan endoxylanase, feraxanase, GH11 endoxylanase, GH115 alpha-glucuronidase, GH30 endoxylanase C, GH30 xylanase, glucuronoxylan xylanohydrolase, glucuronoxylan xylohydrolase, glucuronoxylan-specific xylanase A, glucuronoxylan-specific Xyn30D, glucuronoxylanase, glucuronoxylanase GH30, GuXN 30, M2, M4, TLX, Tx-Xyl, xylanase 30 A, xylanase C, xylanase, glucuronoarabinoxylan endo-1,4-beta-, Xyn30A, Xyn30D, XynA, XynC, XynGH30

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.136 glucuronoarabinoxylan endo-1,4-beta-xylanase

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Results	in table
180	AA Sequence
4	Application
1	CAS Registry Number
9	Cloned(Commentary)
5	Crystallization (Commentary)
3	General Information
1	General Stability
2	Inhibitors
3	KM Value [mM]
2	Localization
2	Metals/Ions
8	Molecular Weight [Da]
1	Natural Substrates/ Products (Substrates)
33	Organism
16	PDB ID
10	pH Optimum
1	pH Range
3	pH Stability
2	pI Value
9	Protein Variants
10	Purification (Commentary)
1	Reaction
6	Reaction Type
31	Reference
4	Source Tissue
9	Specific Activity [micromol/min/mg]
1	Storage Stability
78	Substrates and Products (Substrate)
10	Subunits
65	Synonyms
1	Systematic Name
8	Temperature Optimum [°C]
1	Temperature Range [°C]
5	Temperature Stability [°C]
6	Turnover Number [1/s]

Purification

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Purification on EC 3.2.1.136 - glucuronoarabinoxylan endo-1,4-beta-xylanase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

3 entries

centrifuged supernatant concentrated with membrane filter, applied to Bio-Gel P100 gel filtration column, cellulolytic fractions pooled, lyophilized, dissolved, loaded onto hydroxylapatite column, last steps repeated, 4 degrees Celsius, purity monitored by SDS-PAGE and isoelectric focusing

Cellulomonas flavigena

A1XM14

697503

His Trap column chromatography and Superose 6 gel filtration

Acetivibrio thermocellus

-

745565

MEP HyperCel column chromatography, SP Sepharose column chromatography, and XK 26/20 column chromatography

Dickeya chrysanthemi

Q46961

714978

recombinant His-tagged full-length enzyme and isolated catalytic domain from Escherichia coli strain BL21Star (DE3) by immobilized metal affinity chromatography

Paenibacillus barcinonensis

H6WCZ0

732153

recombinant His-tagged XynC from Escherichia coli strain BL21(DE3) by nickel affinity chromatography

Bacillus subtilis

Q45070

706971

shaking of wheat kernels with acetate buffer (25 mM, pH 5.0, 0.02% sodium azide) for 17 h, liquid phase dialyzed against 10 mM sodium acetate buffer, pH 5.0 at 7 degrees Celsius for 24 h, dialysate concentrated by freeze-drying and resuspension in water

Triticum aestivum

-

680326

to near homogeneity

Bacillus subtilis

-

646828

-

Thermomyces lanuginosus

-

646829

-

Bacillus subtilis subsp. subtilis

Q45070

715930

-

Acetivibrio thermocellus

A3DJS9

738886