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3.2.1.130: glycoprotein endo-alpha-1,2-mannosidase

This is an abbreviated version!
For detailed information about glycoprotein endo-alpha-1,2-mannosidase, go to the full flat file.

Word Map on EC 3.2.1.130

Reaction

(Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6))(Glcalpha(1-3)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-R
+
H2O
=
(Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6))(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-R
 +
Glcalpha(1-3)Man

Synonyms

alpha-1,2-endomannosidase, alpha-endomannosidase, BtGH99, endo-alpha-1,2-mannanase, endo-alpha-1,2-mannosidase, endo-alpha-1->2-mannanase, endo-alpha-D-mannosidase, endo-alpha-mannosidase, endomannosidase, GH99, glucosyl mannosidase, glucosylmannosidase, Golgi endo-a-mannosidase, Golgi-endomannosidase, MANEA

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.130 glycoprotein endo-alpha-1,2-mannosidase

Engineering

Engineering on EC 3.2.1.130 - glycoprotein endo-alpha-1,2-mannosidase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E154A
the mutant shows near zero activity
E329A
the mutant shows near zero activity
E332A
the mutant shows approximate 50fold decrease in catalytic activity with respect to wild type enzyme
E154A
-
the mutant shows near zero activity
-
E329A
-
the mutant shows near zero activity
-
E332A
-
the mutant shows approximate 50fold decrease in catalytic activity with respect to wild type enzyme
-
C188A
-
enzymatic activity can not be fully rescued with a back-engineered Cys188Ala CHO cell endomannosidase
C188S
-
enzymatic activity can not be fully rescued with a back-engineered Cys188Ser CHO cell endomannosidase
C188W
-
enzyme activity can be reversed in cells with a back-engineered Cys188Trp CHO cell endomannosidase, in particular N-glycans of alpha1-antitrypsin become fully processed
W188C
-
molecular defect in CHO cells, is functionally important since it alone results in endoplasmic reticulum mislocalization of endomannosidase and causes greatly reduced in vivo activity
E407D
the mutant has excellent transglycosylation activity and extremely low hydrolytic activity. The minimum motif required for glycosyl acceptor is Man-alpha-(1->2)-Man
W188C
processing of the oligosaccharides of alpha1-antitrypsin is reduced, greatly diminished enzyme activity
additional information
-
in CHO-K1 cells expressing DELTAsig-rEndo, lacking the whole signal sequence, or DELTATMD-rEndo, lacking the transmembrane domain, endomannosidase is undetectable. DELTACT-rEndo, lacking the cytoplasmic tail, exhibits an ER localization and fails to maintain a type II membrane topology