3.2.1.130: glycoprotein endo-alpha-1,2-mannosidase
This is an abbreviated version!
For detailed information about glycoprotein endo-alpha-1,2-mannosidase, go to the full flat file.
Word Map on EC 3.2.1.130
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3.2.1.130
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oligosaccharide
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glucosidase
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n-linked
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deglucosylation
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trim
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glucosylated
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castanospermine
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polymannose
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glucose-substituted
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monoglucosylated
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spiro
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man8glcnac
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1-deoxymannojirimycin
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triglucosylated
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castanospermine-treated
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ii-deficient
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1-deoxynojirimycin
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paranoia
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glc1man9glcnac
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medicine
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synthesis
- 3.2.1.130
- oligosaccharide
-
glucosidase
-
n-linked
-
deglucosylation
-
trim
-
glucosylated
- castanospermine
-
polymannose
-
glucose-substituted
-
monoglucosylated
-
spiro
- man8glcnac
- 1-deoxymannojirimycin
-
triglucosylated
-
castanospermine-treated
-
ii-deficient
- 1-deoxynojirimycin
-
paranoia
-
glc1man9glcnac
- medicine
- synthesis
Reaction
Synonyms
alpha-1,2-endomannosidase, alpha-endomannosidase, BtGH99, endo-alpha-1,2-mannanase, endo-alpha-1,2-mannosidase, endo-alpha-1->2-mannanase, endo-alpha-D-mannosidase, endo-alpha-mannosidase, endomannosidase, GH99, glucosyl mannosidase, glucosylmannosidase, Golgi endo-a-mannosidase, Golgi-endomannosidase, MANEA
ECTree
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Cloned
Cloned on EC 3.2.1.130 - glycoprotein endo-alpha-1,2-mannosidase
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cloning of endomannosidase from CHO-K1 cells, amplicon subcloned into the Hind III/Xho I sites of the expression vector pcDNA6/myc-His, in frame with the C-terminal myc- and His-tag. Myc-tagged CHO endomannosidase expressed in CHO Lec23 cells. Generation of rat/CHO endomannosidase hybrid constructs
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endomannosidase subcloned into the HindIII/XhoI site of the pcDNA6 vector in frame with the myc-tag or into the Hind III/Bam HI site of the pEGFP vector for endomannosidase, expressed in CHO-K1 cells. CHO-K1 cells expressing DELTAsig-rEndo or DELTATMD-rEndo
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expressed in Escherichia coli BL21(DE3) cells
expression in Pichia pastoris
myc-tagged rat endomannosidase expressed in CHO Lec23 cells, generation of rat/CHO endomannosidase hybrid constructs
subcloned into the expression vector pcDNA6/myc-His, overexpression in clone 9 cells and castanospermine-treated hepatocytes