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3.2.1.130: glycoprotein endo-alpha-1,2-mannosidase

This is an abbreviated version!
For detailed information about glycoprotein endo-alpha-1,2-mannosidase, go to the full flat file.

Word Map on EC 3.2.1.130

Reaction

(Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6))(Glcalpha(1-3)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-R
+
H2O
=
(Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6))(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-R
+
Glcalpha(1-3)Man

Synonyms

alpha-1,2-endomannosidase, alpha-endomannosidase, BtGH99, endo-alpha-1,2-mannanase, endo-alpha-1,2-mannosidase, endo-alpha-1->2-mannanase, endo-alpha-D-mannosidase, endo-alpha-mannosidase, endomannosidase, GH99, glucosyl mannosidase, glucosylmannosidase, Golgi endo-a-mannosidase, Golgi-endomannosidase, MANEA

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.130 glycoprotein endo-alpha-1,2-mannosidase

Cloned

Cloned on EC 3.2.1.130 - glycoprotein endo-alpha-1,2-mannosidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA expressed in Escherichia coli
cloning of endomannosidase from CHO-K1 cells, amplicon subcloned into the Hind III/Xho I sites of the expression vector pcDNA6/myc-His, in frame with the C-terminal myc- and His-tag. Myc-tagged CHO endomannosidase expressed in CHO Lec23 cells. Generation of rat/CHO endomannosidase hybrid constructs
-
endomannosidase subcloned into the HindIII/XhoI site of the pcDNA6 vector in frame with the myc-tag or into the Hind III/Bam HI site of the pEGFP vector for endomannosidase, expressed in CHO-K1 cells. CHO-K1 cells expressing DELTAsig-rEndo or DELTATMD-rEndo
-
expresion in Escherichia coli
expressed in CHO-K1 cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli JM109
-
expression in Escherichia coli
expression in Pichia pastoris
myc-tagged rat endomannosidase expressed in CHO Lec23 cells, generation of rat/CHO endomannosidase hybrid constructs
subcloned into the expression vector pcDNA6/myc-His, overexpression in clone 9 cells and castanospermine-treated hepatocytes