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3.2.1.129: endo-alpha-sialidase

This is an abbreviated version!
For detailed information about endo-alpha-sialidase, go to the full flat file.

Word Map on EC 3.2.1.129

Reaction

sialyl-alpha(2-8)sialyl-alpha(2-8)sialyl-alpha(2-8)sialic acid
+
H2O
= 2 sialyl-alpha(2-8)sialic acid

Synonyms

alpha-2,8-sialosylhydrolase, endo-N, endo-N 1588, endo-N-acetylneuraminidase, endo-sialidase, endoE, EndoN Trap, endoN92, endoneuraminidase, endoneuraminidase-N, endoNF, EndoNt, endosialidase, endosialidase E, endosialidase NF, endosialidaseNF, G102, gp47, neuraminidase, endo-, poly(alpha-2,8-sialoside) alpha-2,8-sialosylhydrolase, poly(alpha-2,8-sialosyl) endo-N-acetylneuraminidase, poly(sialoside) alpha-2,8-sialosylhydrolase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.129 endo-alpha-sialidase

Cloned

Cloned on EC 3.2.1.129 - endo-alpha-sialidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21-Gold(DE3) cells
expression in Escherichia coli
expression in Escherichia coli as fusion protein
Vectrevirus K1E
-
expression in Escherichia coli BL21(DE3)
mutant proteins harbouring single amino acid substitutions expressed as GFP-fusion proteins
protein expression in Escherichia coli BL21-Gold(DE3) in the presence of 100 microgram/ml Carbenicillin, 30°C
-
subcloned into the NdeI and XhoI restriction sites of pET22b, resulting in a construct encoding the C-terminal domain of endoNF with a C-terminal His6 tag, expressed in Escherichia coli BL21-Gold(DE3)
-