3.2.1.129: endo-alpha-sialidase
This is an abbreviated version!
For detailed information about endo-alpha-sialidase, go to the full flat file.
Word Map on EC 3.2.1.129
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3.2.1.129
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polysialic
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ncam
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polysialylated
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psa-ncam
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alpha-2,8-linked
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polysias
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bacteriophage-derived
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psa-specific
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polysialyltransferase
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colominic
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neunac
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medicine
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biotechnology
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degradation
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molecular biology
- 3.2.1.129
-
polysialic
- ncam
-
polysialylated
-
psa-ncam
-
alpha-2,8-linked
-
polysias
-
bacteriophage-derived
-
psa-specific
- polysialyltransferase
-
colominic
-
neunac
- medicine
- biotechnology
- degradation
- molecular biology
Reaction
+ = 2 sialyl-alpha(2-8)sialic acid
Synonyms
alpha-2,8-sialosylhydrolase, endo-N, endo-N 1588, endo-N-acetylneuraminidase, endo-sialidase, endoE, EndoN Trap, endoN92, endoneuraminidase, endoneuraminidase-N, endoNF, EndoNt, endosialidase, endosialidase E, endosialidase NF, endosialidaseNF, G102, gp47, neuraminidase, endo-, poly(alpha-2,8-sialoside) alpha-2,8-sialosylhydrolase, poly(alpha-2,8-sialosyl) endo-N-acetylneuraminidase, poly(sialoside) alpha-2,8-sialosylhydrolase
ECTree
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Cloned
Cloned on EC 3.2.1.129 - endo-alpha-sialidase
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expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
mutant proteins harbouring single amino acid substitutions expressed as GFP-fusion proteins
protein expression in Escherichia coli BL21-Gold(DE3) in the presence of 100 microgram/ml Carbenicillin, 30°C
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subcloned into the NdeI and XhoI restriction sites of pET22b, resulting in a construct encoding the C-terminal domain of endoNF with a C-terminal His6 tag, expressed in Escherichia coli BL21-Gold(DE3)
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