3.2.1.123: endoglycosylceramidase

This is an abbreviated version!
For detailed information about endoglycosylceramidase, go to the full flat file.

Reaction

Synonyms

ceramidase, endoglycosyl-, ceramide glycanase, EGALC, EGC, EGCase, EGCase I, EGCase II, EGCase III, EGCII, EGCrP1, EGCrP2, endo-glycoceramidase II, endogalactosylceramidase, endoglycoceramidase, endoglycoceramidase I, endoglycoceramidase II, endoglycoceramidase-related protein, glycosyl-N-acetyl-sphingosine 1,1-beta-D-glucanohydrolase, oligogalactosyl-N-acylsphingosine 1,1'-beta-galactohydrolase

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.123 endoglycosylceramidase

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Results	in table
28	Activating Compound
62	AA Sequence
6	Application
1	CAS Registry Number
14	Cloned(Commentary)
3	Crystallization (Commentary)
5	General Stability
32	Inhibitors
11	kcat/KM [mM/s]
3	Ki Value [mM]
27	KM Value [mM]
6	Localization
5	Metals/Ions
16	Molecular Weight [Da]
5	Natural Substrates/ Products (Substrates)
66	Organism
1	Oxidation Stability
11	PDB ID
25	pH Optimum
3	pH Range
4	pH Stability
6	pI Value
1	Posttranslational Modification
39	Protein Variants
16	Purification (Commentary)
1	Reaction
3	Reaction Type
56	Reference
13	Source Tissue
24	Specific Activity [micromol/min/mg]
3	Storage Stability
239	Substrates and Products (Substrate)
12	Subunits
75	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
1	Temperature Range [°C]
4	Temperature Stability [°C]
14	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 3.2.1.123 - endoglycosylceramidase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

2-N-hexadecanoylamino-4-nitrophenyl-O-beta-lactoside + H2O

N-(2-hydroxy-5-nitrophenyl)hexadecanamide + beta-lactose

2 entries

4-methylumbelliferyl beta-D-glucoside + H2O

4-methylumbelliferone + beta-D-glucose

2 entries

4-nitrophenyl alpha-D-glucoside + H2O

4-nitrophenol + alpha-D-glucose

2 entries

4-nitrophenyl beta-D-glucoside + H2O

4-nitrophenol + beta-D-glucose

2 entries

7-nitro-2,1,3-benzoxadiazole-pentanol + digalactosyldiacylglycerol

?

Rhodococcus equi

-

transglycosylation

703903

-

-

?

alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-beta-D-GalNAc-(1->4)-(alpha-Neu5Ac-(2->3))-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide + H2O

ceramide + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-beta-D-GalNAc-(1->4)-(alpha-Neu5Ac-(2->3))-beta-D-Gal-(1->4)-beta-D-Glc

2 entries

alpha-L-fucosyl-(1->2)-ganglioside GM1a + H2O

?

Rhodococcus equi

-

isoform EGCase I exhibits 100% activity with this substrate

732295

-

-

?

alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide + H2O

ceramide + alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-Glc

3 entries

beta-D-Gal-(1->3)-beta-D-GalNAc-(1->4)-(alpha-Neu5Ac-(2->3))-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide + H2O

ceramide + beta-D-Gal-(1->3)-beta-D-GalNAc-(1->4)-(alpha-Neu5Ac-(2->3))-beta-D-Gal-(1->4)-beta-D-Glc

3 entries

beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide + H2O

ceramide + beta-D-Gal-(1->4)-Glc

3 entries

beta-D-GalNAc-(1->3)-alpha-Gal-(1->4)-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide + H2O

ceramide + beta-D-GalNAc-(1->3)-alpha-Gal-(1->4)-beta-D-Gal-(1->4)-beta-D-Glc

Rhodococcus equi

A0A3S5YBC7

-

751102

-

-

?

blood group A type 1 heptaglycosylceramide + H2O

?

Rhodococcus sp.

-

-

715127

-

-

?

blood group A type 2 heptaglycosylceramide + H2O

?

Rhodococcus sp.

-

-

715127

-

-

?

C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide + H2O

?

2 entries

D-erythro-dihydrosphingosine + H2O

?

Rhodococcus sp.

-

-

680770

-

-

?

D-erythro-sphingosine + H2O

?

Rhodococcus sp.

-

-

680770

-

-

?

Forssman glycolipid + H2O

?

Rhodococcus equi

-

isoform EGCase I exhibits 25.2% activity with this substrate

732295

-

-

?

Fucalpha(1->3)GalNAcalpha(1->3)[Fucalpha(1->2)]Galbeta(1->4)Glcbeta(1-1)ceramide + H2O

Fucalpha(1->3)GalNAcalpha(1->3)[Fucalpha(1->2)]Galbeta(1->4)Glc + ceramide

Rhodococcus sp.

-

84% of the activity with galactosylcerebroside

646687

-

646687

?

Galalpha(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galalpha(1-4)Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

i.e. Gb3Cer

646691

-

-

?

Galalpha1-6Galalpha1-6Galbeta1-6Galbeta1-1'ceramide + H2O

ceramide + Galalpha1-6Galalpha1-6Gal

Rhodococcus equi

-

most rapidly hydrolyzed

680753

-

-

?

Galalpha1-6Galbeta1-3-diacylglycerol + H2O

?

Rhodococcus equi

-

-

703903

-

-

?

Galalpha1-6Galbeta1-6Galbeta1-1'ceramide + H2O

ceramide + Galalpha1-6Galbeta1-6Gal

Rhodococcus equi

-

-

680753

-

-

?

Galbeta(1,3)GalNacbeta(1,4)Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O

ceramide + Galbeta(1,3)GalNacbeta(1,4)Galbeta(1,4)Glc

2 entries

Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O

ceramide + Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glc

10 entries

Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,8)NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O

ceramide + Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,8)NeuAcalpha(2,3)]Galbeta(1,4)Glc

2 entries

Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O

ceramide + Galbeta(1,4)Glc

2 entries

Galbeta(1,6)Galbeta(1,6)Galbeta-1,1'-ceramide + H2O

?

2 entries

Galbeta(1,6)Galbeta(1,6)Glcbeta-1,1'-ceramide + H2O

ceramide + Galbeta(1,6)Galbeta(1,6)Glc

Hydra vulgaris

-

37°C, pH 3.0, 0.2% (w/v) Triton X-100

665547

-

-

?

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

15 entries

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + methanol

?

Cyanea nozakii

-

i.e. GM1

646694

products may be attributed to GM-1 oligosaccharide II3NeuAcGgOse4 and methyl-GM-1 oligosaccharide methyl-II3NeuAcGgOse4

646694

?

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide 4-nitrobenzo-2-oxy-1,3-diazole + H2O

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + 4-nitrobenzo-2-oxy-1,3-diazole ceramide

2 entries

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

5 entries

Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glc + ceramide

7 entries

Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galbeta(1-4)Glc + ceramide

6 entries

Galbeta(1-6)Galbeta(1-6)Galbeta(1-1)ceramide + H2O

Galbeta(1-6)Galbeta(1-6)Gal + ceramide

Rhodococcus sp.

-

i.e. neogalactosylceramide, 77% of the activity with lactosylceramide

646687

-

646687

?

Galbeta1-1'-ceramide + H2O

?

Rhodococcus equi

-

-

703903

-

-

?

Galbeta1-6Galbeta1-1'-ceramide + H2O

?

Rhodococcus equi

-

highest transglycosylation efficiency

703903

-

-

?

Galbeta1-6Galbeta1-1'ceramide + H2O

ceramide + Galbeta1-6Galbeta

Rhodococcus equi

-

-

680753

-

-

?

Galbeta1-6Galbeta1-6Galbeta1-1'-ceramide + H2O

?

Rhodococcus equi

-

-

703903

-

-

?

Galbeta1-6Galbeta1-6Galbeta1-1'-ceramide + H2O

ceramide + Galbeta1-6Galbeta1-6Gal

Rhodococcus equi

-

-

680753

-

-

?

Galbeta1-6Galbeta1-6Galbeta1-6Galbeta1-1'-ceramide + H2O

?

Rhodococcus equi

-

-

703903

-

-

?

GalNAcalpha(1,4)GalNAcbeta(1,4)GlcNAcbeta(1,3)Manbeta(1,4)Glcbeta-1,1'-ceramide + H2O

ceramide + GalNAcalpha(1,4)GalNAcbeta(1,4)GlcNAcbeta(1,3)Manbeta(1,4)Glc

Hydra vulgaris

-

37°C, pH 3.0, 0.2% (w/v) Triton X-100

665547

-

-

?

GalNAcalpha(1-3)GalNAcbeta(1-3)Galalpha(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

GalNAcalpha(1-3)GalNAcbeta(1-3)Galalpha(1-4)Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

i.e. Gb5Cer

646691

-

-

?

GalNAcbeta(1,3)Galalpha(1,4)Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O

ceramide + GalNAcbeta(1,3)Galalpha(1,4)Galbeta(1,4)Glc

Hydra vulgaris

-

37°C, pH 3.0, 0.2% (w/v) Triton X-100

665547

-

-

?

GalNAcbeta(1-3)Galalpha(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

GalNAcbeta(1-3)Galalpha(1-4)Galbeta(1-4)Glc + ceramide

4 entries

GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

3 entries

GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

GalNAcbeta(1-4)Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

i.e. Gg3Cer

646693

-

-

?

ganglioside + H2O

?

Rhodococcus sp.

-

-

680770

-

-

?

ganglioside fucosyl-GM1 + H2O

?

Rhodococcus sp.

-

100% hydrolyzed by EGCase I and 19% hydrolyzed by EGCase II

698135

-

-

?

ganglioside Gb3Cer + H2O

?

Rhodococcus equi

-

isoform EGCase I exhibits 100% activity with this substrate

732295

-

-

?

ganglioside Gb4Cer + H2O

?

Rhodococcus equi

-

isoform EGCase I exhibits 33.9% activity with this substrate

732295

-

-

?

ganglioside GbOse3Cer + H2O

?

Rhodococcus sp.

-

73% hydrolyzed by EGCase I and 24% hydrolyzed by EGCase II

698135

-

-

?

ganglioside GbOse4Cer + H2O

?

Rhodococcus sp.

-

34% hydrolyzed by EGCase I and 7% hydrolyzed by EGCase II

698135

-

-

?

ganglioside GbOse5Cer + H2O

?

Rhodococcus sp.

-

25% hydrolyzed by EGCase I and 6.5% hydrolyzed by EGCase II

698135

-

-

?

ganglioside GD1a + H2O

?

3 entries

ganglioside GD1b + H2O

?

3 entries

ganglioside GD3 + H2O

?

3 entries

ganglioside GM1 + H2O

?

2 entries

ganglioside GM1a + H2O

?

Rhodococcus equi

-

isoform EGCase I exhibits 100% activity with this substrate

732295

-

-

?

ganglioside GM3 + H2O

?

Rhodococcus equi

-

isoform EGCase I exhibits 100% activity with this substrate

732295

-

-

?

ganglioside TGC + H2O

?

Rhodococcus sp.

-

66% hydrolyzed by EGCase III

698135

-

-

?

GlcA4Me-beta(1,4)[GalNAc-Me-alpha(1,3)]Fucalpha(1,4)GlcNAcbeta(1,2)Manalpha(1,3)[Xylbeta(1,2)]Manbeta(1,4)Glcbeta-1,1'-ceramide + H2O

ceramide + GlcA4Me-beta(1,4)[GalNAc-Me-alpha(1,3)]Fucalpha(1,4)GlcNAcbeta(1,2)Manalpha(1,3)[Xylbeta(1,2)]Manbeta(1,4)Glc

Hydra vulgaris

-

37°C, pH 3.0, 0.2% (w/v) Triton X-100

665547

-

-

?

Glcbeta1-6Galbeta1-1'-ceramide + H2O

?

Rhodococcus equi

-

-

703903

-

-

?

GlcNAcalpha1-2Fucalpha1-3Manbeta1-4Glcbeta1-1'-ceramide + H2O

ceramide + GlcNAcalpha1-2Fucalpha1-3Manbeta1-4Glc

Rhodococcus sp.

-

-

703891

-

-

?

GlcNAcbeta(1-2)Manalpha(1-3)Manbeta(1-4)Glcbeta(1-1)ceramide + H2O

GlcNAcbeta(1-2)Manalpha(1-3)Manbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

35% of the activity with lactosylceramide

646687

-

646687

?

globotetraosylceramide + H2O

?

Rhodococcus sp.

-

-

715127

-

-

?

globotriaosylceramide + H2O

?

Rhodococcus sp.

-

-

715127

-

-

?

glucosylceramide + H2O

?

Rhodococcus equi

-

isoform EGCase I exhibits 6.7% activity with this substrate

732295

-

-

?

glycosphingolipid + H2O

?

11 entries

GM1 ganglioside + H2O

GM1-aglycan + beta-D-Gal-(1->3)-beta-D-GalNAc-(1->4)-(alpha-Neu5Ac-(2->3))-beta-D-Gal-(1->4)-beta-D-Glc

Rhodococcus sp. M-777

O33853

-

750248

-

-

?

isoglobotetraosylceramide + H2O

?

Rhodococcus sp.

-

-

715127

-

-

?

isoglobotriaosylceramide + H2O

?

Rhodococcus sp.

-

-

715127

-

-

?

lactosylceramide + H2O

?

Rhodococcus equi

-

isoform EGCase I exhibits 29.9% activity with this substrate

732295

-

-

?

Manalpha(1-3)Manbeta(1-4)Glcbeta(1-1)ceramide + H2O

Manalpha(1-3)Manbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

88% of the activity with lactosylceramide

646687

-

646687

?

Manbeta(1-4)Glcbeta(1-1)ceramide + H2O

Manbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

93% of the activity with lactosylceramide

646687

-

646687

?

monogalactosylceramide + H2O

ceramide + D-galactose

Rhodococcus equi

-

-

680753

-

-

?

NeuAcalpha(2,3)Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O

ceramide + NeuAcalpha(2,3)Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glc

Hydra vulgaris

-

37°C, pH 3.0, 0.2% (w/v) Triton X-100

665547

-

-

?

NeuAcalpha(2,3)Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,8)NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O

ceramide + NeuAcalpha(2,3)Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,8)NeuAcalpha-2,3]Gal-beta(1,4)Glc

Hydra vulgaris

-

37°C, pH 3.0, 0.2% (w/v) Triton X-100, most favoured substrate

665547

-

-

?

NeuAcalpha(2,3)Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O

ceramide + NeuAcalpha(2,3)Galbeta(1,4)Glc

Hydra vulgaris

-

37°C, pH 3.0, 0.2% (w/v) Triton X-100

665547

-

-

?

NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

4 entries

NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

5 entries

NeuAcalpha(2-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

NeuAcalpha(2-3)Galbeta(1-4)Glc + ceramide

5 entries

NeuAcalpha(2-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

NeuAcalpha(2-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc + ceramide

4 entries

NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

GT, 81% of the activity with lactosylceramide

646687

-

646687

?

NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

4 entries

NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-4)Glc + ceramide

2 entries

non-acid glycosphingolipids of human gastric epithelium + H2O

?

Rhodococcus sp.

-

-

715127

-

-

?

oligoglycosylglucosyl-(1<->1)-ceramide + H2O

ceramide + oligoglycosylglucose

Rhodococcus equi

-

-

732295

-

-

?

porcine brain ganglioside-1 + H2O

?

Sus scrofa

-

-

680549

-

-

?

porcine brain ganglioside-3 + H2O

?

Sus scrofa

-

-

680549

-

-

?

porcine brain ganglioside-4 + H2O

?

Sus scrofa

-

-

680549

-

-

?

porcine brain ganglioside-5 + H2O

?

Sus scrofa

-

-

680549

-

-

?

resorufin + beta-D-glucose + H2O

resorufin-beta-D-glucoside

2 entries

trigalactosylceramide + H2O

?

Rhodococcus equi

-

-

703903

-

-

?

additional information

?

-

36 entries

2-N-hexadecanoylamino-4-nitrophenyl-O-beta-lactoside + H2O

N-(2-hydroxy-5-nitrophenyl)hexadecanamide + beta-lactose

Corynebacterium sp.

-

-

646700

-

-

?

2-N-hexadecanoylamino-4-nitrophenyl-O-beta-lactoside + H2O

N-(2-hydroxy-5-nitrophenyl)hexadecanamide + beta-lactose

Rhodococcus sp.

-

derivative possessing an N-acyl chain, 4-nitrophenyllactoside chromogenic substrate C16ampNPLac

646700

-

-

?

4-methylumbelliferyl beta-D-glucoside + H2O

4-methylumbelliferone + beta-D-glucose

Cryptococcus neoformans

-

-

732178

-

-

?

4-methylumbelliferyl beta-D-glucoside + H2O

4-methylumbelliferone + beta-D-glucose

Cryptococcus neoformans H99

-

-

732178

-

-

?

4-nitrophenyl alpha-D-glucoside + H2O

4-nitrophenol + alpha-D-glucose

Cryptococcus neoformans

-

low activity

732178

-

-

?

4-nitrophenyl alpha-D-glucoside + H2O

4-nitrophenol + alpha-D-glucose

Cryptococcus neoformans H99

-

low activity

732178

-

-

?

4-nitrophenyl beta-D-glucoside + H2O

4-nitrophenol + beta-D-glucose

Cryptococcus neoformans

-

high activity

732178

-

-

?

4-nitrophenyl beta-D-glucoside + H2O

4-nitrophenol + beta-D-glucose

Cryptococcus neoformans H99

-

high activity

732178

-

-

?

alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-beta-D-GalNAc-(1->4)-(alpha-Neu5Ac-(2->3))-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide + H2O

ceramide + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-beta-D-GalNAc-(1->4)-(alpha-Neu5Ac-(2->3))-beta-D-Gal-(1->4)-beta-D-Glc

Rhodococcus equi

A0A3S5YBC7

-

751102

-

-

?

alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-beta-D-GalNAc-(1->4)-(alpha-Neu5Ac-(2->3))-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide + H2O

ceramide + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-beta-D-GalNAc-(1->4)-(alpha-Neu5Ac-(2->3))-beta-D-Gal-(1->4)-beta-D-Glc

Rhodococcus equi 103S

A0A3S5YBC7

-

751102

-

-

?

alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide + H2O

ceramide + alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-Glc

Rhodococcus equi

A0A3S5YBC7

-

751102

-

-

?

alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide + H2O

ceramide + alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-Glc

Rhodococcus equi 103S

A0A3S5YBC7

-

751102

-

-

?

alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide + H2O

ceramide + alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-Glc

Rhodococcus sp. M-777

O33853

preferred substrate

751102

-

-

?

beta-D-Gal-(1->3)-beta-D-GalNAc-(1->4)-(alpha-Neu5Ac-(2->3))-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide + H2O

ceramide + beta-D-Gal-(1->3)-beta-D-GalNAc-(1->4)-(alpha-Neu5Ac-(2->3))-beta-D-Gal-(1->4)-beta-D-Glc

Rhodococcus equi

A0A3S5YBC7

-

751102

-

-

?

beta-D-Gal-(1->3)-beta-D-GalNAc-(1->4)-(alpha-Neu5Ac-(2->3))-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide + H2O

ceramide + beta-D-Gal-(1->3)-beta-D-GalNAc-(1->4)-(alpha-Neu5Ac-(2->3))-beta-D-Gal-(1->4)-beta-D-Glc

Rhodococcus equi 103S

A0A3S5YBC7

-

751102

-

-

?

beta-D-Gal-(1->3)-beta-D-GalNAc-(1->4)-(alpha-Neu5Ac-(2->3))-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide + H2O

ceramide + beta-D-Gal-(1->3)-beta-D-GalNAc-(1->4)-(alpha-Neu5Ac-(2->3))-beta-D-Gal-(1->4)-beta-D-Glc

Rhodococcus sp. M-777

O33853

-

751102

-

-

?

beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide + H2O

ceramide + beta-D-Gal-(1->4)-Glc

Rhodococcus equi

A0A3S5YBC7

-

751102

-

-

?

beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide + H2O

ceramide + beta-D-Gal-(1->4)-Glc

Rhodococcus equi 103S

A0A3S5YBC7

-

751102

-

-

?

beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide + H2O

ceramide + beta-D-Gal-(1->4)-Glc

Rhodococcus sp. M-777

O33853

-

751102

-

-

?

C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide + H2O

?

Cryptococcus neoformans

-

-

732178

-

-

?

C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide + H2O

?

Cryptococcus neoformans H99

-

-

732178

-

-

?

Galbeta(1,3)GalNacbeta(1,4)Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O

ceramide + Galbeta(1,3)GalNacbeta(1,4)Galbeta(1,4)Glc

Hydra vulgaris

-

37°C, pH 3.0, 0.2% (w/v) Triton X-100

665547

-

-

?

Galbeta(1,3)GalNacbeta(1,4)Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O

ceramide + Galbeta(1,3)GalNacbeta(1,4)Galbeta(1,4)Glc

Hydra vulgaris 105

-

37°C, pH 3.0, 0.2% (w/v) Triton X-100

665547

-

-

?

Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O

ceramide + Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glc

Aiptasia sp.

-

37°C, pH 3.0, 0.2% (w/v) Triton X-100

665547

-

-

?

Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O

ceramide + Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glc

Bacillus sp. (in: Bacteria)

-

37°C, pH 3.0, 0.2% (w/v) Triton X-100

665547

-

-

?

Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O

ceramide + Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glc

Cellana sp.

-

37°C, pH 3.0, 0.2% (w/v) Triton X-100

665547

-

-

?

Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O

ceramide + Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glc

Cyanea nozakii

Q9GV16

37°C, pH 3.0, 0.2% (w/v) Triton X-100

665547

-

-

?

Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O

ceramide + Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glc

Entacmaea sp.

-

37°C, pH 3.0, 0.2% (w/v) Triton X-100

665547

-

-

?

Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O

ceramide + Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glc

Hydra vulgaris

-

37°C, pH 3.0, 0.2% (w/v) Triton X-100

665547

-

-

?

Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O

ceramide + Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glc

Hydra vulgaris 105

-

37°C, pH 3.0, 0.2% (w/v) Triton X-100

665547

-

-

?

Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O

ceramide + Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glc

Marphysa sp.

-

37°C, pH 3.0, 0.2% (w/v) Triton X-100

665547

-

-

?

Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O

ceramide + Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glc

Meretrix sp.

-

37°C, pH 3.0, 0.2% (w/v) Triton X-100

665547

-

-

?

Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O

ceramide + Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glc

Ruditapes sp.

-

37°C, pH 3.0, 0.2% (w/v) Triton X-100

665547

-

-

?

Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,8)NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O

ceramide + Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,8)NeuAcalpha(2,3)]Galbeta(1,4)Glc

Hydra vulgaris

-

37°C, pH 3.0, 0.2% (w/v) Triton X-100

665547

-

-

?

Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,8)NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O

ceramide + Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,8)NeuAcalpha(2,3)]Galbeta(1,4)Glc

Hydra vulgaris 105

-

37°C, pH 3.0, 0.2% (w/v) Triton X-100

665547

-

-

?

Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O

ceramide + Galbeta(1,4)Glc

Hydra vulgaris

-

37°C, pH 3.0, 0.2% (w/v) Triton X-100

665547

-

-

?

Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O

ceramide + Galbeta(1,4)Glc

Hydra vulgaris 105

-

37°C, pH 3.0, 0.2% (w/v) Triton X-100

665547

-

-

?

Galbeta(1,6)Galbeta(1,6)Galbeta-1,1'-ceramide + H2O

?

Hydra vulgaris

-

37°C, pH 3.0, 0.2% (w/v) Triton X-100, no reaction

665547

-

-

?

Galbeta(1,6)Galbeta(1,6)Galbeta-1,1'-ceramide + H2O

?

Hydra vulgaris 105

-

37°C, pH 3.0, 0.2% (w/v) Triton X-100, no reaction

665547

-

-

?

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

Condylactis sp.

-

i.e. GM1

646699

-

-

?

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

Corynebacterium sp.

-

i.e. GM1

646688, 646703

-

-

?

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

Corynebacterium sp.

-

cholera toxin receptor

646703

-

-

?

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

Cyanea nozakii

Q9GV16

i.e. GM1

646695

-

-

?

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

i.e. GM1

646687

-

646687

?

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

i.e. GM1

646690, 646691

-

-

?

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

i.e. GM1

646699

-

646699

?

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

i.e. GM1

646702

-

-

?

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

O33853

i.e. GM1

646696

-

-

?

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

GM1a

646699

-

646699

?

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

O33853

GM1a

646696

-

-

?

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

as active as lactosylceramide

646687

-

646687

?

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

Rhodococcus sp. C9

-

i.e. GM1

646702

-

-

?

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

Rhodococcus sp. G-74-2

-

i.e. GM1

646687

-

646687

?

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

Rhodococcus sp. G-74-2

-

as active as lactosylceramide

646687

-

646687

?

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide 4-nitrobenzo-2-oxy-1,3-diazole + H2O

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + 4-nitrobenzo-2-oxy-1,3-diazole ceramide

Rhodococcus sp.

-

i.e. NBD-GM1

646701

-

646701

?

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide 4-nitrobenzo-2-oxy-1,3-diazole + H2O

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + 4-nitrobenzo-2-oxy-1,3-diazole ceramide

Rhodococcus sp. C9

-

i.e. NBD-GM1

646701

-

646701

?

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

Cyanea nozakii

Q9GV16

GD1b

646695

-

-

?

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

GD1b

646702

-

-

?

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

good substrate for strain M-777, weak substrate for strain C9

646702

-

-

?

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

Rhodococcus sp. C9

-

GD1b

646702

-

-

?

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

Rhodococcus sp. C9

-

good substrate for strain M-777, weak substrate for strain C9

646702

-

-

?

Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glc + ceramide

Cyanea nozakii

Q9GV16

weak substrate

646695

-

-

?

Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glc + ceramide

Cyanea nozakii

Q9GV16

i.e. asialo-GM1

646695

-

-

?

Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

as active as lactosylceramide

646687

-

646687

?

Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

i.e. asialo-GM1

646687

-

646687

?

Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

i.e. asialo-GM1

646690, 646691, 646692, 646702

-

-

?

Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

O33853

i.e. asialo-GM1

646696

-

-

?

Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glc + ceramide

Rhodococcus sp. C9

-

i.e. asialo-GM1

646702

-

-

?

Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galbeta(1-4)Glc + ceramide

Cyanea nozakii

Q9GV16

weak substrate

646695

-

-

?

Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galbeta(1-4)Glc + ceramide

Cyanea nozakii

Q9GV16

i.e. lactosylceramide

646695

-

-

?

Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galbeta(1-4)Glc + ceramide

Cyanea nozakii

-

i.e. lactosylceramide

646694

i.e. lactose

646694

r

Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

i.e. lactosylceramide

646687

-

646687

?

Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

i.e. lactosylceramide

646691, 646697, 646702

-

-

?

Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

O33853

i.e. lactosylceramide

646696

-

-

?

GalNAcbeta(1-3)Galalpha(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

GalNAcbeta(1-3)Galalpha(1-4)Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

O33853

weak substrate

646696

-

-

?

GalNAcbeta(1-3)Galalpha(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

GalNAcbeta(1-3)Galalpha(1-4)Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

i.e. Gb4Cer

646691

-

-

?

GalNAcbeta(1-3)Galalpha(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

GalNAcbeta(1-3)Galalpha(1-4)Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

i.e. Gb4Cer

646699

-

646699

?

GalNAcbeta(1-3)Galalpha(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

GalNAcbeta(1-3)Galalpha(1-4)Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

O33853

i.e. Gb4Cer

646696

-

-

?

GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

-

646691, 646702

-

-

?

GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

O33853

-

646696

-

-

?

GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

GM2, 94% of the activity with lactosylceramide

646687

-

646687

?

ganglioside GD1a + H2O

?

Rhodococcus equi

-

isoform EGCase I exhibits 100% activity with this substrate

732295

-

-

?

ganglioside GD1a + H2O

?

Rhodococcus sp.

-

100% hydrolyzed by EGCase I and II

698135

-

-

?

ganglioside GD1a + H2O

?

Rhodococcus sp. M-750

-

100% hydrolyzed by EGCase I and II

698135

-

-

?

ganglioside GD1b + H2O

?

Rhodococcus equi

-

isoform EGCase I exhibits 100% activity with this substrate

732295

-

-

?

ganglioside GD1b + H2O

?

Rhodococcus sp.

-

87% hydrolyzed by EGCase I and 74% hydrolyzed by EGCase II

698135

-

-

?

ganglioside GD1b + H2O

?

Rhodococcus sp. M-750

-

87% hydrolyzed by EGCase I and 74% hydrolyzed by EGCase II

698135

-

-

?

ganglioside GD3 + H2O

?

Rhodococcus equi

-

isoform EGCase I exhibits 100% activity with this substrate

732295

-

-

?

ganglioside GD3 + H2O

?

Rhodococcus sp.

-

100% hydrolyzed by EGCase I and II

698135

-

-

?

ganglioside GD3 + H2O

?

Rhodococcus sp. M-750

-

100% hydrolyzed by EGCase I and II

698135

-

-

?

ganglioside GM1 + H2O

?

Rhodococcus sp.

-

100% hydrolyzed by EGCase I and II

698135

-

-

?

ganglioside GM1 + H2O

?

Rhodococcus sp. M-750

-

100% hydrolyzed by EGCase I and II

698135

-

-

?

glycosphingolipid + H2O

?

Corynebacterium sp.

-

glycosphingolipids readily hydrolyzed, globo-type (slowly)

646688

-

-

?

glycosphingolipid + H2O

?

Corynebacterium sp.

-

ganglio-type

646688

-

-

?

glycosphingolipid + H2O

?

Corynebacterium sp.

-

neutral and acidic in presence of 0.1% Triton X-100, lacto-type

646688

-

-

?

glycosphingolipid + H2O

?

Corynebacterium sp.

-

glycosphingolipids, overview

646688

-

-

?

glycosphingolipid + H2O

?

Rhodococcus sp.

-

strongly resistant to hydrolysis by endoglycoceramidase II in comparison with endoglycoceramidase I

646691

-

-

?

glycosphingolipid + H2O

?

Rhodococcus sp.

-

ganglio-type

646691

-

-

?

glycosphingolipid + H2O

?

Rhodococcus sp.

-

GM3

646690

-

-

?

glycosphingolipid + H2O

?

Rhodococcus sp.

-

neutral and acidic in presence of 0.1% Triton X-100, lacto-type

646691

-

-

?

glycosphingolipid + H2O

?

Rhodococcus sp.

-

GM2

646690

-

-

?

glycosphingolipid + H2O

?

Rhodococcus sp.

-

cell-surface glycosphingolipids of erythrocytes, horse-erythrocyte cell-surface GM3 and guinea pig erythrocyte cell-surface Gg3Cer

646693

-

-

?

glycosphingolipid + H2O

?

Rhodococcus sp.

-

release of glycans from the ceramide moieties of glycosphingolipids by endoglycoceramidase II treatment in order to analyze structures of glycosphingolipids in normal human colorectal epithelial cells, and characteristic alterations of oligosaccharide structures in malignant transformation

699823

-

-

?

NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

-

646691

-

-

?

NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

GD, 93% of the activity with lactosylceramide

646687

-

646687

?

NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

GD1a

646702

-

-

?

NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

O33853

GD1a

646696

-

-

?

NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

Cyanea nozakii

Q9GV16

GT1b

646695

-

-

?

NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

-

646691

-

646691

?

NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

good substrate for strain M-777, weak substrate for strain C9

646702

-

-

?

NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

GT1b

646687, 646702

-

-

?

NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

O33853

GT1b

646696

-

-

?

NeuAcalpha(2-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

NeuAcalpha(2-3)Galbeta(1-4)Glc + ceramide

Cyanea nozakii

Q9GV16

i.e. GM3

646695

-

-

?

NeuAcalpha(2-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

NeuAcalpha(2-3)Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

93% of the activity with lactosylceramide

646687

-

646687

?

NeuAcalpha(2-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

NeuAcalpha(2-3)Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

i.e. GM3

646687

-

646687

?

NeuAcalpha(2-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

NeuAcalpha(2-3)Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

i.e. GM3

646690, 646691, 646693, 646697, 646702

-

-

?

NeuAcalpha(2-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

NeuAcalpha(2-3)Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

O33853

i.e. GM3

646696

-

-

?

NeuAcalpha(2-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

NeuAcalpha(2-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc + ceramide

Corynebacterium sp.

-

-

646688

-

-

?

NeuAcalpha(2-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

NeuAcalpha(2-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

-

646691

-

-

?

NeuAcalpha(2-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

NeuAcalpha(2-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

O33853

-

646696

-

-

?

NeuAcalpha(2-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

NeuAcalpha(2-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

i.e. sialylparagloboside, as active as lactosylceramide

646687

-

646687

?

NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

Cyanea nozakii

Q9GV16

GQ1b

646695

-

-

?

NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

-

646691

-

646691

?

NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

good substrate for strain M-777, weak substrate for strain C9

646702

-

-

?

NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

GQ1b

646702

-

-

?

NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-4)Glc + ceramide

Cyanea nozakii

Q9GV16

GD3

646695

-

-

?

NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O

NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-4)Glc + ceramide

Rhodococcus sp.

-

GD3

646702

-

-

?

resorufin + beta-D-glucose + H2O

resorufin-beta-D-glucoside

Cryptococcus neoformans

-

highest catalytic efficiency

732178

-

-

?

resorufin + beta-D-glucose + H2O

resorufin-beta-D-glucoside

Cryptococcus neoformans H99

-

highest catalytic efficiency

732178

-

-

?

additional information

?

-

Corynebacterium sp.

-

enzyme has transglycosylation activity, 1-hexanol is best acceptor for GM1, 1-pentanol and 1-heptanol are also used

646689

-

-

?

additional information

?

-

Corynebacterium sp.

-

enzyme prefers lactosides with acylamide structures over simple n-alkyl lactosides as substrates

646700

-

-

?

additional information

?

-

Corynebacterium sp.

-

enzyme destroys the cholera toxin receptor GM1 on the cell surface of HELA cells and therefore reduces the amount of cholera toxin attached to the cells

646703

-

-

?

additional information

?

-

Corynebacterium sp. A-809

-

enzyme has transglycosylation activity, 1-hexanol is best acceptor for GM1, 1-pentanol and 1-heptanol are also used

646689

-

-

?

additional information

?

-

Rhodococcus equi

-

does not hydrolyze ganglio-, globo-, or lacto-series glycosphingolipids

680753

-

-

?

additional information

?

-

Rhodococcus equi

-

EGALC specifically hydrolyzes the Galbeta1-1'-ceramide linkage in 6-Gal series glycosphingolipids

703903

-

-

?

additional information

?

-

Rhodococcus equi

-

no activity towards Galbeta1-6Glcbeta1-1'-ceramide, Galbeta1-4Galbeta1-1'-ceramide, Galalpha1-1'ceramide, Glcbeta1-1'-ceramide, Galbeta1-4Glcbeta1-1'ceramide, Galalpha1-4Galbeta1-4Glcbeta1-1'-ceramide, and Galbeta1-3GalNAcbeta1-4(NeuAcalpha2-3)Galbeta1-4Glcbeta1-1'-ceramide

703903

-

-

?

additional information

?

-

Rhodococcus equi

-

almost no hydrolysis is observed for isoform EGCase II with alpha-L-fucosyl-(1->2)-ganglioside GM1a, ganglioside Gb3Cer, and ganglioside Gb4Cer. Isoform EGCase I shows no activity with ganglioside GM4, trigalactosylceramide, sulfatide, and sphingomyelin

732295

-

-

?

additional information

?

-

Rhodococcus equi

-

for transglycosylation activity, isoform EGCase I prefers alkanols with a relatively long carbon chain such as pentanol and hexanol as an acceptor substrate

732295

-

-

?

additional information

?

-

Rhodococcus equi

A0A3S5YBC7

specifically hydrolyzes the glycosidic linkage between the oligosaccharide and the ceramide moieties of various glycosphingolipids. No activity with beta-D-Glc-(1<->1')-ceramide and beta-D-Gal-(1<->1')-ceramide

751102

-

-

?

additional information

?

-

Rhodococcus equi 103S

A0A3S5YBC7

specifically hydrolyzes the glycosidic linkage between the oligosaccharide and the ceramide moieties of various glycosphingolipids. No activity with beta-D-Glc-(1<->1')-ceramide and beta-D-Gal-(1<->1')-ceramide

751102

-

-

?

additional information

?

-

Rhodococcus sp.

-

no substrate: GalNAcbeta(1-3)Galalpha(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide, i.e. Gb4Cer

646698

-

-

?

additional information

?

-

Rhodococcus sp.

-

no substrate: sulfatides

646691

-

-

?

additional information

?

-

Rhodococcus sp.

-

no substrate: sphingomyelins

646691

-

-

?

additional information

?

-

Rhodococcus sp.

-

no substrate: gala-type glycosphingolipids

646691

-

-

?

additional information

?

-

Rhodococcus sp.

-

enzyme prefers lactosides with acylamide structures over simple n-alkyl lactosides as substrates

646700

-

-

?

additional information

?

-

Rhodococcus sp.

-

no substrate: cerebrosides

646691

-

-

?

additional information

?

-

Rhodococcus sp.

-

no substrate: glycoglycerolipids

646691

-

-

?

additional information

?

-

Rhodococcus sp.

-

no substrate: Galalpha(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O, i.e. Gb3Cer

646698

-

-

?

additional information

?

-

Rhodococcus sp.

-

no substrate: N-linked glycoproteins

646687

-

-

?

additional information

?

-

Rhodococcus sp.

-

no substrate: O-linked glycoproteins

646687

-

-

?

additional information

?

-

Rhodococcus sp.

-

hardly attacks linkages between monosaccharides and ceramides, i.e. cerebrosides, or between oligosaccharides and diacylglycerol, i.e. glycoglycerolipids

646687

-

-

?

additional information

?

-

Rhodococcus sp.

-

enzyme from strain C9 hydrolyzes b-series gangliotetraosylceramides more slowly than M-777 enzyme, whereas both enzymes hydrolyse a-series gangliosides and neutral glycosphingolipids to the same extent

646702

-

-

?

additional information

?

-

Rhodococcus sp.

-

reduces glycosphingolipid levels in HL60 cells

646697

-

-

?

additional information

?

-

Rhodococcus sp.

-

removes glycosphinolipid-sugar chains from the cell surface of living cells

646698

-

-

?

additional information

?

-

Rhodococcus sp.

-

reduces ganglioside content in membrane fractions and intact cells of A431 cells

646698

-

-

?

additional information

?

-

Rhodococcus sp.

-

acidic glycosphingolipids from primary colonic adenocarcinoma and hepatic metastasis, releases glycans from the ceramide moiety

677532

-

-

?

additional information

?

-

Rhodococcus sp.

-

both rhodococcal EGCases I and II efficiently hydrolyze ganglio-series glycosphingolipids, whereas EGCase I hydrolyzes globo-series-glycosphingolipids much faster than EGCase II. The glycan chain from fucosyl-GM1 can be prepared by using EGCase I. EGCase III stands alone to hydrolyze 6-gala-series glycosphingolipids. All EGCases are not able to hydrolyze sphingomyelin and sulfatide

698135

-

-

?

additional information

?

-

Rhodococcus sp.

-

synthesis of sugar-sphingolipid glycosidic linkages. Extent of ganglioside synthesis is proportional to enzyme concentration and length of incubation time

695545

-

-

?

additional information

?

-

Rhodococcus sp. C9

-

enzyme from strain C9 hydrolyzes b-series gangliotetraosylceramides more slowly than M-777 enzyme, whereas both enzymes hydrolyse a-series gangliosides and neutral glycosphingolipids to the same extent

646702

-

-

?

additional information

?

-

Rhodococcus sp. G-74-2

-

no substrate: N-linked glycoproteins

646687

-

-

?

additional information

?

-

Rhodococcus sp. G-74-2

-

no substrate: O-linked glycoproteins

646687

-

-

?

additional information

?

-

Rhodococcus sp. G-74-2

-

hardly attacks linkages between monosaccharides and ceramides, i.e. cerebrosides, or between oligosaccharides and diacylglycerol, i.e. glycoglycerolipids

646687

-

-

?

additional information

?

-

Rhodococcus sp. M-750

-

both rhodococcal EGCases I and II efficiently hydrolyze ganglio-series glycosphingolipids, whereas EGCase I hydrolyzes globo-series-glycosphingolipids much faster than EGCase II. The glycan chain from fucosyl-GM1 can be prepared by using EGCase I. EGCase III stands alone to hydrolyze 6-gala-series glycosphingolipids. All EGCases are not able to hydrolyze sphingomyelin and sulfatide

698135

-

-

?

additional information

?

-

Rhodococcus sp. M-777

O33853

no activity with beta-D-Glc-(1<->1')-ceramide, beta-D-Gal-(1<->1')-ceramide, beta-D-GalNAc-(1->3)-alpha-Gal-(1->4)-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide and alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-beta-D-GalNAc-(1->4)-(alpha-Neu5Ac-(2->3))-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide

751102

-

-

?

additional information

?

-

Sus scrofa

-

Fucalpha(1-2)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3)Galbeta(1-4))Glcbeta(1-1)ceramide is strongly resistant to hydrolysis by the enzyme

680549

-

-

?