3.2.1.123: endoglycosylceramidase
This is an abbreviated version!
For detailed information about endoglycosylceramidase, go to the full flat file.
Word Map on EC 3.2.1.123
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3.2.1.123
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glycosphingolipids
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gangliosides
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cgases
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n-deacylase
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yamagata
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ganglio
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macrobdella
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decora
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gm1a
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diagnostics
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analysis
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drug development
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medicine
- 3.2.1.123
- glycosphingolipids
- gangliosides
- cgases
-
n-deacylase
-
yamagata
-
ganglio
-
macrobdella
- decora
- gm1a
- diagnostics
- analysis
- drug development
- medicine
Reaction
Synonyms
ceramidase, endoglycosyl-, ceramide glycanase, EGALC, EGC, EGCase, EGCase I, EGCase II, EGCase III, EGCII, EGCrP1, EGCrP2, endo-glycoceramidase II, endogalactosylceramidase, endoglycoceramidase, endoglycoceramidase I, endoglycoceramidase II, endoglycoceramidase-related protein, glycosyl-N-acetyl-sphingosine 1,1-beta-D-glucanohydrolase, oligogalactosyl-N-acylsphingosine 1,1'-beta-galactohydrolase
ECTree
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Cloned
Cloned on EC 3.2.1.123 - endoglycosylceramidase
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amplified gene product encoding amino acid residues 23-488 without the signal peptide digested with HindIII and NotI and inserted into the corresponding sites of pET22b(+) to generate a C-terminal His6-tagged protein. The recombinant plasmid and mutants expressed in Escherichia coli strain BL21(DE3)
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expressed in Escherichia coli BL21(DE3) cells
mutant E351S in pET28a subcloned into pTKNd and pUC18 at the NdeI/SalI and EcoRI/SalI restriction sites, respectively. EGC and its mutants expressed in Escherichia coli BL21 (Tuner) cells
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recombinant EGC, lacking the 30 amino acid N-terminal signal sequence overexpressed in Escherichia coli
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subcloned into pET28a using the NdeI/XhoI restriction sites and expressed in Escherichia coli BL21
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