3.2.1.123: endoglycosylceramidase
This is an abbreviated version!
For detailed information about endoglycosylceramidase, go to the full flat file.
Word Map on EC 3.2.1.123
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3.2.1.123
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glycosphingolipids
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gangliosides
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cgases
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n-deacylase
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yamagata
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ganglio
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macrobdella
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decora
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gm1a
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diagnostics
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analysis
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drug development
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medicine
- 3.2.1.123
- glycosphingolipids
- gangliosides
- cgases
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n-deacylase
-
yamagata
-
ganglio
-
macrobdella
- decora
- gm1a
- diagnostics
- analysis
- drug development
- medicine
Reaction
Synonyms
ceramidase, endoglycosyl-, ceramide glycanase, EGALC, EGC, EGCase, EGCase I, EGCase II, EGCase III, EGCII, EGCrP1, EGCrP2, endo-glycoceramidase II, endogalactosylceramidase, endoglycoceramidase, endoglycoceramidase I, endoglycoceramidase II, endoglycoceramidase-related protein, glycosyl-N-acetyl-sphingosine 1,1-beta-D-glucanohydrolase, oligogalactosyl-N-acylsphingosine 1,1'-beta-galactohydrolase
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Activating Compound
Activating Compound on EC 3.2.1.123 - endoglycosylceramidase
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dimethylformamide
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the activity is enhanced by addition of 5% (v/v) dimethylformamide
Dimethylsulfoxide
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the activity is enhanced by addition of 10% (v/v) dimethylsulfoxide
sodium taurodeoxycholate
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activates endoglycoceramidase I and II, inhibition of endoglycoceramidase I above 0.4% w/v
tetrahydrofuran
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the activity is enhanced by addition of 5% (v/v) tetrahydrofuran
additional information
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for maximal hydrolysis of glycosphingolipids by rhodococcal EGCases, a detergent such as Triton X-100 is required at a final concentration of 0.1-0.3% of the reaction mixture. The hydrolysis of water soluble gangliosides such as GM1 does not require the presence of a detergent. For the hydrolysis of GM1, the addition of 5% tetrahydrofuran instead of Triton X-100 increases the reaction rate of EGCase I by 2fold compared to that in the absence of a detergent. Tetrahydrofuran is ineffective in facilitating the hydrolysis of GbOse4Cer by EGCase I. DMSO, ethyl acetate, and methyl cellosolve are also not effective in stimulating the hydrolysis of GM1 or GbOse4Cer by EGCase I in the absence of a detergent
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purified from culture supernatant of Rhodococcus sp. M-777, enhances the hydrolysis of glycosphingolipids in vitro and on the cell-surface by EGCase in absence of detergent
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Lubrol Px
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optimal concentration for C9 enzyme: 0.1%, no activation of M-777 enzyme
Triton X-100
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the activity is enhanced by addition of 0.1% (v/v) Triton X-100