3.2.1.123: endoglycosylceramidase

This is an abbreviated version!
For detailed information about endoglycosylceramidase, go to the full flat file.

Word Map on EC 3.2.1.123

3.2.1.123

glycosphingolipids

gangliosides

cgases

n-deacylase

yamagata

ganglio

macrobdella

decora

gm1a

diagnostics

analysis

drug development

medicine

Reaction

Synonyms

ceramidase, endoglycosyl-, ceramide glycanase, EGALC, EGC, EGCase, EGCase I, EGCase II, EGCase III, EGCII, EGCrP1, EGCrP2, endo-glycoceramidase II, endogalactosylceramidase, endoglycoceramidase, endoglycoceramidase I, endoglycoceramidase II, endoglycoceramidase-related protein, glycosyl-N-acetyl-sphingosine 1,1-beta-D-glucanohydrolase, oligogalactosyl-N-acylsphingosine 1,1'-beta-galactohydrolase

ECTree

3 Hydrolases

3.2 Glycosylases

3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

3.2.1.123 endoglycosylceramidase

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Results	in table
28	Activating Compound
62	AA Sequence
6	Application
1	CAS Registry Number
14	Cloned(Commentary)
3	Crystallization (Commentary)
5	General Stability
32	Inhibitors
11	kcat/KM [mM/s]
3	Ki Value [mM]
27	KM Value [mM]
6	Localization
5	Metals/Ions
16	Molecular Weight [Da]
5	Natural Substrates/ Products (Substrates)
66	Organism
1	Oxidation Stability
11	PDB ID
25	pH Optimum
3	pH Range
4	pH Stability
6	pI Value
1	Posttranslational Modification
39	Protein Variants
16	Purification (Commentary)
1	Reaction
3	Reaction Type
56	Reference
13	Source Tissue
24	Specific Activity [micromol/min/mg]
3	Storage Stability
239	Substrates and Products (Substrate)
12	Subunits
75	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
1	Temperature Range [°C]
4	Temperature Stability [°C]
14	Turnover Number [1/s]

Activating Compound

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Activating Compound on EC 3.2.1.123 - endoglycosylceramidase

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Activator protein

4 entries

detergent

2 entries

dimethylformamide

Rhodococcus equi

the activity is enhanced by addition of 5% (v/v) dimethylformamide

732295

Dimethylsulfoxide

Rhodococcus equi

the activity is enhanced by addition of 10% (v/v) dimethylsulfoxide

Lubrol Px

Nonidet P-40

sodium cholate

sodium taurodeoxycholate

Rhodococcus sp.

activates endoglycoceramidase I and II, inhibition of endoglycoceramidase I above 0.4% w/v

646691

taurodeoxycholate

Rhodococcus sp.

0.5 mg/ml, 2.5fold increase of activity

646687

tetrahydrofuran

Rhodococcus equi

the activity is enhanced by addition of 5% (v/v) tetrahydrofuran

732295

Triton X-100

8 entries

additional information

Rhodococcus sp.

for maximal hydrolysis of glycosphingolipids by rhodococcal EGCases, a detergent such as Triton X-100 is required at a final concentration of 0.1-0.3% of the reaction mixture. The hydrolysis of water soluble gangliosides such as GM1 does not require the presence of a detergent. For the hydrolysis of GM1, the addition of 5% tetrahydrofuran instead of Triton X-100 increases the reaction rate of EGCase I by 2fold compared to that in the absence of a detergent. Tetrahydrofuran is ineffective in facilitating the hydrolysis of GbOse4Cer by EGCase I. DMSO, ethyl acetate, and methyl cellosolve are also not effective in stimulating the hydrolysis of GM1 or GbOse4Cer by EGCase I in the absence of a detergent

698135