3.2.1.115: branched-dextran exo-1,2-alpha-glucosidase

This is an abbreviated version!
For detailed information about branched-dextran exo-1,2-alpha-glucosidase, go to the full flat file.

Reaction

Synonyms

dextran 1,2-alpha glucosidase, dextran alpha-(1-->2)-debranching enzyme, dextran alpha-1,2 debranching enzyme, dextranase I, dextranhydrolase, glucosidase, dextran 1,2-alpha

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.115 branched-dextran exo-1,2-alpha-glucosidase

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Results	in table
1	Activating Compound
1	CAS Registry Number
7	Inhibitors
3	KM Value [mM]
2	Molecular Weight [Da]
1	Natural Substrates/ Products (Substrates)
2	Organism
2	pH Optimum
1	pH Stability
2	Purification (Commentary)
1	Reaction
2	Reaction Type
2	Reference
4	Specific Activity [micromol/min/mg]
11	Substrates and Products (Substrate)
1	Subunits
6	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Stability [°C]

Substrates Products

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Substrates Products on EC 3.2.1.115 - branched-dextran exo-1,2-alpha-glucosidase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

2(3),2(4)-di-O-alpha-glucosyl-isomaltotetraose + H2O

isomaltotetraose + 2 alpha-D-glucose

Flavobacterium sp.

-

-

697134

-

-

?

2(3)-O-alpha-D-glucosyl-isomaltotetraose + H2O

isomaltotetraose + alpha-D-glucose

Flavobacterium sp.

-

-

697134

-

-

?

2(3)-O-alpha-D-glucosyl-isomaltotriose + H2O

isomaltotriose + alpha-D-glucose

Flavobacterium sp.

-

-

697134

-

-

?

B-1298 soluble dextran + H2O

D-glucose + ?

Flavobacterium sp.

-

incubation at 40°C for 24 h, specifically splitz alpha-1,2-glucosidic linkages

697134

-

-

?

B-1299 soluble dextran + H2O

D-glucose + ?

Flavobacterium sp.

-

incubation at 40°C for 24 h

697134

-

-

?

B-1397 soluble dextran + H2O

D-glucose + ?

Flavobacterium sp.

-

incubation at 40°C for 24 h

697134

-

-

?

dextran + H2O

alpha-D-glucose

2 entries

mutan IG-1 + H2O

?

Flavobacterium sp.

-

major linkages: alpha-1,3-, alpha-1,6-, 18% as effective as soluble dextran for induction of enzyme

646645

-

-

?

additional information

?

-

2 entries

dextran + H2O

alpha-D-glucose

Flavobacterium sp.

-

B-1299, soluble, major linkages: alpha-1,6-, alpha-1,3-, alpha-1,2-, most effective substrate for induction of enzyme

646645

hydrolysis of 1,2-alpha-D-glucosidic linkages at the branched points, producing free D-glucose

646645

?

dextran + H2O

alpha-D-glucose

Flavobacterium sp.

-

clinical dextran, major linkages: alpha-1,6-, alpha-1,3-, 55% as effective as soluble dextran for induction of enzyme

646645

hydrolysis of 1,2-alpha-D-glucosidic linkages at the branched points, producing free D-glucose

646645

?

additional information

?

-

Flavobacterium sp.

-

effect of different carbon sources on enzyme activity, no activity with alpha-1,3-glucan, starch, glucose or sucrose

646645

-

-

?

additional information

?

-

Flavobacterium sp.

-

hydrolysis of alpha-1,2-glucosidic linkages at branching points of dextran

646645

-

-

?