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3.2.1.114: mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase

This is an abbreviated version!
For detailed information about mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase, go to the full flat file.

Word Map on EC 3.2.1.114

Reaction

Man5GlcNAc3-[protein]
+ 2 H2O =
Man3GlcNAc3-[protein]
 + 2 alpha-D-mannopyranose

Synonyms

1,3(1,6)-alpha-D-mannosidase, 1,6-alpha-D-mannosidase, Afams1, alpha-1,3 mannosidase, alpha-1,3-mannosidase, alpha-D-mannosidase II, alpha-mannosidase, alpha-mannosidase II, alpha-mannosidase III, alpha-mannosidase IIx, alpha-MII, alpha1-3,6-mannosidase, Bt3991, class II alpha-D-mannosidase, class II alpha-mannosidase, class IIC alpha-mannosidase, dGMII, Drosophila GMII, exo-1,3-1,6-alpha-mannosidase, GH38 alpha-mannosidase II, GH38 enzyme, GH38 Golgi alpha-mannosidase II, GlcNAc transferase I-dependent alpha1,3[alpha1,6]mannosidase, glycoside hydrolase, GmII, Golgi alpha-mannosidase II, Golgi mannosidase IIx, human type II alpha-mannosidase, jack bean alpha-mannosidase, Man II, MAN IIx, MAN2A1, ManII, ManIII, mannose-trimming enzyme, mannosidase II, mannosidase, exo-1,3-1,6-alpha-, mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase, mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase II, MII, More, NAM, neutral alpha-mannosidase, SpGH38, TM1851

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.114 mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase

Storage Stability

Storage Stability on EC 3.2.1.114 - mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C or 4°C, 10 mM sodium acetate buffer, pH 5, with or without CaCl2, 4 months, stable
-
-20°C or 4°C, 4 months, less than 5% loss of activity
-
-20°C, in 10 mM HEPES/NaOH, pH 7.5, 2 mM MgCl2, 0.1% Nonidet-P40, 0.2 M NaCl, less than 10% loss of activity within 3 months
-
0-4°C, 10 mM HEPES buffer, pH 7.1, 0.1% Triton X-100, 0.5 mM PMSF, 0.2 mM dithiothreitol, 10% glycerol, at least 2 weeks, stable
-
0-4°C, 10 mM HEPES buffer, pH 7.2, 10% glycerol, several days, stable
-
2-4°C, 10 mM potassium phosphate buffer, pH 7.2, 1% Triton X-100, 4 weeks, 20% loss of activity
-
4°C, column-bound glutathione S-transferase-tagged secreted form of SfManIII, at least 1 week, stable
-
4°C, intact enzyme, several weeks, stable
-
5°C or frozen, 0.1 M potassium phosphate buffer, pH 7.2, Triton X-100, 30 days, 20% loss of activity
-
frozen, 10 mM HEPES buffer, pH 7.1, 0.1% Triton X-100, 0.5 mM PMSF, 0.2 mM dithiothreitol, 10% glycerol, at least 2 months, stable
-
frozen, at least 2 months, stable
-