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3.2.1.114: mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase

This is an abbreviated version!
For detailed information about mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase, go to the full flat file.

Word Map on EC 3.2.1.114

Reaction

Man5GlcNAc3-[protein]
+ 2 H2O =
Man3GlcNAc3-[protein]
 + 2 alpha-D-mannopyranose

Synonyms

1,3(1,6)-alpha-D-mannosidase, 1,6-alpha-D-mannosidase, Afams1, alpha-1,3 mannosidase, alpha-1,3-mannosidase, alpha-D-mannosidase II, alpha-mannosidase, alpha-mannosidase II, alpha-mannosidase III, alpha-mannosidase IIx, alpha-MII, alpha1-3,6-mannosidase, Bt3991, class II alpha-D-mannosidase, class II alpha-mannosidase, class IIC alpha-mannosidase, dGMII, Drosophila GMII, exo-1,3-1,6-alpha-mannosidase, GH38 alpha-mannosidase II, GH38 enzyme, GH38 Golgi alpha-mannosidase II, GlcNAc transferase I-dependent alpha1,3[alpha1,6]mannosidase, glycoside hydrolase, GmII, Golgi alpha-mannosidase II, Golgi mannosidase IIx, human type II alpha-mannosidase, jack bean alpha-mannosidase, Man II, MAN IIx, MAN2A1, ManII, ManIII, mannose-trimming enzyme, mannosidase II, mannosidase, exo-1,3-1,6-alpha-, mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase, mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase II, MII, More, NAM, neutral alpha-mannosidase, SpGH38, TM1851

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.114 mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase

Purification

Purification on EC 3.2.1.114 - mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
1200fold
-
ammonium sulfate fractionation of recombinant enzyme followed by two cobalt chelating chromatography steps
-
anion-exchange chromatography, chromatofocusing chromatography, gel filtration
-
glutathione S-transferase-tagged secreted form of SfManIII
-
intact 124 kDa Man II and chymotrypsin-cleaved soluble 110 kDa catalytic subunit
-
method not mentioned
-
native enzyme 600fold from mature seeds to homogeneity by ammonium sulfate fractionation, ion exchange and hydrophobic interaction chromatography, and gel filtration, and another 2 steps of ion exchange chromatography and gel filtration
-
native enzyme from mature seeds to homogeneity by ammonium sulfate fractionation, ion exchange and hydrophobic interaction chromatography, and gel filtration
-
partial
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant protein A fusion of alpha-mannosidase II and IIx, expressed in COS cells
-
recombinant secreted His-tagged soluble enzyme from culture medium of Spodoptera frugiperda Sf21 insect cells by nickel affinity chromatography
recombinant TM1851 from Escherichia coli by ion exchange and hydroxyapatite chromatography, and gel filtration
-
soluble alpha-mannosidase IIx protein purified by a nickel affinity column
-