3.2.1.114: mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase

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For detailed information about mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase, go to the full flat file.

Reaction

Synonyms

1,3(1,6)-alpha-D-mannosidase, 1,6-alpha-D-mannosidase, Afams1, alpha-1,3 mannosidase, alpha-1,3-mannosidase, alpha-D-mannosidase II, alpha-mannosidase, alpha-mannosidase II, alpha-mannosidase III, alpha-mannosidase IIx, alpha-MII, alpha1-3,6-mannosidase, Bt3991, class II alpha-D-mannosidase, class II alpha-mannosidase, class IIC alpha-mannosidase, dGMII, Drosophila GMII, exo-1,3-1,6-alpha-mannosidase, GH38 alpha-mannosidase II, GH38 enzyme, GH38 Golgi alpha-mannosidase II, GlcNAc transferase I-dependent alpha1,3[alpha1,6]mannosidase, glycoside hydrolase, GmII, Golgi alpha-mannosidase II, Golgi mannosidase IIx, human type II alpha-mannosidase, jack bean alpha-mannosidase, Man II, MAN IIx, MAN2A1, ManII, ManIII, mannose-trimming enzyme, mannosidase II, mannosidase, exo-1,3-1,6-alpha-, mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase, mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase II, MII, More, NAM, neutral alpha-mannosidase, SpGH38, TM1851

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.114 mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase

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Results	in table
12	Activating Compound
28	AA Sequence
11	Application
2	CAS Registry Number
18	Cloned(Commentary)
18	Crystallization (Commentary)
78	Disease/ Diagnostics
1	Expression
8	General Information
10	General Stability
43	IC50 Value
232	Inhibitors
6	kcat/KM [mM/s]
73	Ki Value [mM]
19	KM Value [mM]
70	Localization
71	Metals/Ions
37	Molecular Weight [Da]
39	Natural Substrates/ Products (Substrates)
96	Organism
5	Pathway
67	PDB ID
31	pH Optimum
8	pH Range
10	pH Stability
5	pI Value
21	Posttranslational Modification
14	Protein Variants
30	Purification (Commentary)
5	Reaction
6	Reaction Type
91	Reference
82	Source Tissue
16	Specific Activity [micromol/min/mg]
11	Storage Stability
219	Substrates and Products (Substrate)
30	Subunits
108	Synonyms
1	Systematic Name
37	Temperature Optimum [°C]
2	Temperature Range [°C]
5	Temperature Stability [°C]
7	Turnover Number [1/s]

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Cloned on EC 3.2.1.114 - mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

alpha-mannosidase II and IIx, expression in COS cells as protein A fusion, overexpression of alpha-mannosidase IIx in CHO-K1 cells

Homo sapiens

-

646641

alpha-mannosidase III cDNA is cloned

Spodoptera frugiperda

-

646644

cDNA fragments encoding full-length enzymes subcloned into the pcDNA3.1 mammalian expression vector. cDNA fragments encoding soluble forms of enzymes subcloned into pcDNA-HSH. Full-length alpha-mannosidase IIx expressed in MII/alpha-mannosidase IIx double-null fibroblasts. Soluble recombinant alpha-mannosidase IIx expressed in COS cells

Mus musculus

-

682539

DNA and amino acid sequence determination and analysis

Aspergillus fumigatus

Q5I5K3

708612

expressed in Drosophila melanogaster

Homo sapiens

-

714909

expressed in HEK293 cells

Homo sapiens

-

664599

expressed in S2 insect cells

Drosophila melanogaster

-

731694

expressed it in Pichia pastoris expression system

Capra hircus

V9XY47

749611

full-length cDNA is cloned and sequenced, 8-12fold overexpression in COS cells

Mus musculus

P27046

646631

gene M1 GAS SF370, DNA and amino acid sequence determination and analysis, cloning of the coding sequence for SpGH38 residues 1-901, overexpression as 3C protease cleavable N-terminal His6-tagged enzyme in Escherichia coli strain BL21(DE3)

Streptococcus pyogenes M1 GAS

Q99YP5

710392

gene MAN2A1 from mutant strain Lec36 and wild-type gene, DNA and amino acid sequence determination and analysis. Wild-type and mutant enzyme expression analysis, overview

Homo sapiens

-

709035

GmII is cloned, sequenced and localized to a single site, 85D14-18, on the right arm of chromosome 3

Drosophila melanogaster

Q24451

646633

GMII, DNA and amino acid sequence determination and analysis, expression of His-tagged functional Golgi alpha-mannosidase II as soluble protein, which is secreted to the culture medium, in Spodoptera frugiperda Sf21 insect cells using the baculovirus transfection system, transient expression of GFP-tagged enzyme in Nicotiana benthamiana plants shows localization of the recombinant enzyme to the Golgi and endoplasmic reticulum

Arabidopsis thaliana

Q9LFR0

666601

Golgi mannosidase II gene is located on chromosome 5q21, Golgi mannosidase IIx on chromosome 15q25

Homo sapiens

-

646639

isolation of partial cDNA clones, gene is located on chromosome 5

Homo sapiens

-

646631

pcDNA-HSH-MX expression vector encoding the soluble alpha-mannosidase IIx, transfection into Cos-1 cells

Mus musculus

-

681767

preparation of expression construct containing 6 x His and tobacco etch virus protease recognition sites in the N-terminus of NAM and integrated into the Pichia pastoris chromosome under inducible alcohol oxygenase promoter. CHO-K1 cells transfected with pNAM-EGFP

Homo sapiens

-

680041

TM1851, expression in Escherichia coli

Thermotoga maritima

-

663538