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3.2.1.11: dextranase

This is an abbreviated version!
For detailed information about dextranase, go to the full flat file.

Word Map on EC 3.2.1.11

Reaction

Dextran
+
H2O
=
dextran fragment 1
 +
dextran fragment 2

Synonyms

1,6-alpha-D-glucan-6-glucanohydrolase, 6-alpha-D-glucan 6-glucanohydrolase, alpha-1,6-D-glucan 6-glucanohydrolase, alpha-1,6-D-glucan-6-glucanohydrolase, Alpha-1,6-glucan-6-glucanohydrolase, alpha-D-1,6-glucan-6-glucanohydrolase, alpha-dextranase, alpha-glucanase, AODex, Dex, Dex2, Dex410, Dex49A, DexA, dextran hydrolase, dextranase, dextranase DL 2, dextranase I, dextranase II, Dextranase Plus L, dextransucrase, DL 2, DXSR, endo-dextranase, endodextranase, extracellular dextranase, Fjoh_4430, LSD1, More, rDex, SmDex, Teth39_0264, TLDex, TPDex

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.11 dextranase

Cloned

Cloned on EC 3.2.1.11 - dextranase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
dex1 gene from Dex70-1B and Dex70-34, expression in Escherichia coli TOP10F
-
dex1 gene from strain Dex40-8 and strain Dex50-2, expression in Escherichia coli TOP10F
-
DNA and amino acid sequence determination and analysis, sequence comparisons, expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) CodonPlus-RIL
expressed in Escherichia coli BL21(DE3) cells
expressed in Pichia pastoris strain GS115
-
expressed in Saccharomyces cerevisiae INVSc1 via fusion of the DNA segment encoding the mature dextranase protein with alpha-factor signal sequence, and insertion into the GAL1-controlled expression vector pYES2/CT (construction of the four dextranase-expressing plasmids pYES2/CT-dex, pYES2/CT-dex-tag, pYES2/CT-alphaF-dex(m) and pYES2/CT-alphaF-dex(m)-tag). Dextranase construct pYESCT2-alphaF-dex(m) introduced into a strain of Saccharomyces cerevisiae expressing the human cytochrome P4503A4 (CYP3A4) and the cognate reductase
expression in Escherichia coli
expression in Pichia pastoris
gene aodex, DNA and amino acid sequence determination and analysis, expression of the His-tagged enzyme in Escherichia coli
gene dex2, expression of the engineered fusion enzyme DSXR in Escherichia coli strain BL21(DE3)pLysS, optimization of protein expression conditions for enhancement of protein production by the effects of three-level-three-factors and their mutual interaction in Escherichia coli
into vector pET-21a(+), recombinant form with six histidine residues tagged in the C-terminus expressed in Escherichia coli strain BL21(DE3)
into vector pPIC9k-His6 and expressed in Pichia pastoris GS115 strain under the control of AOX1 promoter
-
isoenzyme lsd1, expression in Saccharomyces cerevisiae INVSc1
-
the dextransucrase-dextranase fusion protein is expressed in Escherichia coli BL21(DE3) cells
wild-type dex1 gene directly cloned into NotI and XbaI sites of pEH75 and transformed into Escherichia coli Mach1TM-T1 cells. Dex1 coding regions from wild-type and thermostable mutants PCR-amplified from pEH75 and cloned into pET-45b(+) and transformed into Escherichia coli expression host BL21(DE3)