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3.13.1.3: 2'-hydroxybiphenyl-2-sulfinate desulfinase

This is an abbreviated version!
For detailed information about 2'-hydroxybiphenyl-2-sulfinate desulfinase, go to the full flat file.

Word Map on EC 3.13.1.3

Reaction

2'-hydroxybiphenyl-2-sulfinate
+
H2O
=
2-Hydroxybiphenyl
 +
sulfite

Synonyms

2'-hydroxybiphenyl-2-sulfinate desulfinase, 2'-hydroxybiphenyl-2-sulfinic acid desulfinase, 2-(2'-hydroxyphenyl)benzene sulfinate desulfinase, 2-(2'-hydroxyphenyl)benzene sulfinate hydrolase, 2-(2'-hydroxyphenyl)benzenesulfinate desulfinase, 2-(2-hydroxyphenyl)benzenesulfinate:H2O hydrolase, Desulfinase, DszB, EC 3.1.2.24, gene dszB-encoded hydrolase, hydrolase, 2,(2'-hydroxyphenyl)benzene sulfinate, TdsB

ECTree

     3 Hydrolases
         3.13 Acting on carbon-sulfur bonds
             3.13.1 Acting on carbon-sulfur bonds
                3.13.1.3 2'-hydroxybiphenyl-2-sulfinate desulfinase

Engineering

Engineering on EC 3.13.1.3 - 2'-hydroxybiphenyl-2-sulfinate desulfinase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C26S
-
site-directed mutagenesis of the only cysteine residue results in an completely inactive mutant
H60Q
-
mutation causes an 17fold reduction in the specific activity
Q65H
increase in thermostability, decrease in catalytic activity
R70I
-
inactive mutant mostly exists in the insoluble fraction of the cell extract
R70K
-
inactive mutant mostly exists in the insoluble fraction of the cell extract
Y63A
increase in catalytic activity
Y63F
increase in catalytic activity, similar thermostability as wild-type
Y63F/Q65H
increase in thermostability without loss of catalytic activity or affinity for substrate
Y63K
decrease in activity
Y63S
affinity for the substrate is about 7-fold lower than that of the wild-type enzyme
Y63W
activity similar to wild-type
Q65H
Rhodococcus erythropolis IGTS8 / ATCC 53968
-
increase in thermostability, decrease in catalytic activity
-
Y63A
Rhodococcus erythropolis IGTS8 / ATCC 53968
-
increase in catalytic activity
-
Y63F
Rhodococcus erythropolis IGTS8 / ATCC 53968
-
increase in catalytic activity, similar thermostability as wild-type
-
Y63S
Rhodococcus erythropolis IGTS8 / ATCC 53968
-
affinity for the substrate is about 7-fold lower than that of the wild-type enzyme
-
Y63W
Rhodococcus erythropolis IGTS8 / ATCC 53968
-
activity similar to wild-type
-
C26S
-
site-directed mutagenesis of the only cysteine residue results in an completely inactive mutant
-