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3.12.1.B1: tetrathionate hydrolase

This is an abbreviated version!
For detailed information about tetrathionate hydrolase, go to the full flat file.

Word Map on EC 3.12.1.B1

Reaction

tetrathionate
+
H2O
=
thiosulfate
+
sulfate
+
sulfur
+ 2 H+

Synonyms

4THase, Af-Tth, Ahos_1670, SH-tth, Teth, tetrathionate hydrolase, Tth1

ECTree

     3 Hydrolases
         3.12 Acting on sulfur-sulfur bonds
             3.12.1 Acting on sulfur-sulfur bonds (only sub-subclass identified to date)
                3.12.1.B1 tetrathionate hydrolase

Purification

Purification on EC 3.12.1.B1 - tetrathionate hydrolase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli results in the formation of inclusion bodies of the protein in an inactive form. The recombinant protein can be successfully activated after an in vitro refolding treatment. The enzyme requires exposure to an acidicenvironment during protein folding for activation
Q0KK37
high salt concentrations are needed to release enzyme activity during cell disruption
-
partial
recombinant inactive wild-type and mutant enzymes refolded from Escherichia coli strain BL21 Star™(DE3) inclusion bodies