3.1.99.B2: XPF endonuclease
This is an abbreviated version!
For detailed information about XPF endonuclease, go to the full flat file.
Word Map on EC 3.1.99.B2
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3.1.99.B2
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structure-specific
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endonucleases
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flap
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fanconi
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sulfolobus
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fork
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interstrand
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crosslinks
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anemia
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uv-induced
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eukaryal
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stalled
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slide
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nucleases
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xpf-ercc1
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nicked
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crossover
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incises
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helicase
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sliding
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helix-hairpin-helix
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meiotic
- 3.1.99.B2
-
structure-specific
- endonucleases
- flap
-
fanconi
- sulfolobus
-
fork
-
interstrand
-
crosslinks
- anemia
-
uv-induced
-
eukaryal
-
stalled
-
slide
- nucleases
-
xpf-ercc1
-
nicked
-
crossover
-
incises
- helicase
-
sliding
-
helix-hairpin-helix
-
meiotic
Reaction
The best substrate for the structure-specific endonuclease is a 3' flap followed by a nicked duplex, nicked three-way junctions, and nicked fourway junctions. The enzyme from Sulfolobus is only active in the presence of the sliding clamp PCNA (Proliferating-Cell-Nuclear-Antigen). It cleaves the replication fork or 3'-flap structures in the homodimeric form. =
Synonyms
SsoXPF, xeroderma pigmentosum group F endonuclease, XPF endonuclease, XPF repair endonuclease
ECTree
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Substrates Products
Substrates Products on EC 3.1.99.B2 - XPF endonuclease
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REACTION DIAGRAM
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importance of the downstream duplex in directing the endonuclease activity
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additional information
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Sulfolobus XPF is only active in the presence of the sliding clamp PCNA (Proliferating-Cell-Nuclear-Antigen), which is a heterotrimer in this organism. Interactions with two of the three subunits of PCNA are mediated via a C-terminal interaction motif. The PCNA-XPF complex acts as a structure-specific nuclease on a range of DNA flap, bubble and junction substrates. The best substrate is a 3' flap structure, which is cut too quickly to allow accurate determination of the reaction rate using manual sampling methods. This rate is estimated as 6.8 per. The splayed duplex is cleaved at a rate of 0.60 per min, at least 10fold more slowly than the 3' flap substrate, and the fixed four-way junction is cut with a rate of 0.057 per min, 10-fold more slowly than the splayed duplex and 100fold more slowly than the flap substrate. As the four-way junction is cut in all four arms, the true rate of cleavage of this substrate is probably fourfold faster
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additional information
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the best substrate is a 3' flap followed by a nicked duplex, nicked three-way junctions, and nicked fourway junctions. The efficient cleavage of a nicked duplex substrate demonstrates that no single-stranded flap is required, rather a discontinuity in the DNA duplex is enough to promote the nuclease activity
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additional information
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the best substrate is a 3' flap followed by a nicked duplex, nicked three-way junctions, and nicked fourway junctions. The efficient cleavage of a nicked duplex substrate demonstrates that no single-stranded flap is required, rather a discontinuity in the DNA duplex is enough to promote the nuclease activity
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additional information
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the best substrate is a 3' flap followed by a nicked duplex, nicked three-way junctions, and nicked fourway junctions. The efficient cleavage of a nicked duplex substrate demonstrates that no single-stranded flap is required, rather a discontinuity in the DNA duplex is enough to promote the nuclease activity
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-
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additional information
?
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Sulfolobus XPF is only active in the presence of the sliding clamp PCNA (Proliferating-Cell-Nuclear-Antigen), which is a heterotrimer in this organism. Interactions with two of the three subunits of PCNA are mediated via a C-terminal interaction motif. The PCNA-XPF complex acts as a structure-specific nuclease on a range of DNA flap, bubble and junction substrates. The best substrate is a 3' flap structure, which is cut too quickly to allow accurate determination of the reaction rate using manual sampling methods. This rate is estimated as 6.8 per. The splayed duplex is cleaved at a rate of 0.60 per min, at least 10fold more slowly than the 3' flap substrate, and the fixed four-way junction is cut with a rate of 0.057 per min, 10-fold more slowly than the splayed duplex and 100fold more slowly than the flap substrate. As the four-way junction is cut in all four arms, the true rate of cleavage of this substrate is probably fourfold faster
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additional information
?
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importance of the downstream duplex in directing the endonuclease activity
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?