3.1.99.B2: XPF endonuclease

This is an abbreviated version, for detailed information about XPF endonuclease, go to the full flat file.

Reaction

The best substrate for the structure-specific endonuclease is a 3' flap followed by a nicked duplex, nicked three-way junctions, and nicked fourway junctions. The enzyme from Sulfolobus is only active in the presence of the sliding clamp PCNA (Proliferating-Cell-Nuclear-Antigen). It cleaves the replication fork or 3'-flap structures in the homodimeric form. =

Synonyms

SsoXPF, xeroderma pigmentosum group F endonuclease, XPF endonuclease, XPF repair endonuclease

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
            
                3.1.99.B2 XPF endonuclease