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3.1.8.2: diisopropyl-fluorophosphatase

This is an abbreviated version!
For detailed information about diisopropyl-fluorophosphatase, go to the full flat file.

Word Map on EC 3.1.8.2

Reaction

diisopropyl fluorophosphate
+
H2O
=
diisopropyl phosphate
+
fluoride

Synonyms

DFPase, di-isopropylphosphorofluoridate fluorohydrolase, dialkylfluorophosphatase, diisopropyl fluorophosphatase, diisopropyl phosphorofluoridate hydrolase, diisopropylfluorophosphatase, diisopropylfluorophosphonate dehalogenase, diisopropylphosphofluoridase, EC 3.8.2.1, h-PON1, isopropylphosphorofluoridase, More, OP-hydrolyzing enzyme, OPA anhydrase, OPA anhydrolase, OPAA, OPAA-2, opd, OPH, organophosphate acid anhydrase, organophosphate anhydrolase/prolidase, organophosphorus acid anhydrolase, organophosphorus hydrolase, organophosphorus-hydrolyzing enzyme, paraoxonase 1, phosphotriesterase, PON1, PTES5, RC, regucalcin, senescence marker protein-30, SMP30, somanase, tabunase

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.8 Phosphoric-triester hydrolases
                3.1.8.2 diisopropyl-fluorophosphatase

Specific Activity

Specific Activity on EC 3.1.8.2 - diisopropyl-fluorophosphatase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.527
0.998
with diisopropyl phosphorofluoridate as substrate, pH 8.0, 25°C, 1 mM MgCl2
1.512
105
mutant F173V
106
mutant R146S
110
substrate sarin, pH 8.0, temperature not specified in the publication
124
mutant H287F
129
mutant H287W
13.8
purified recombinant PON1 wild-type enzyme, pH 10.5, 25°C, substrate diisopropyl fluorophosphate
154
mutant H287L
17.95
purified recombinant PON1-hFc fusion enzyme, pH 10.5, 25°C, substrate diisopropyl fluorophosphate
173
mutant T195L
174
mutant Q77Y
187
mutant N237S
19
mutant H287A
193
mutant Q77W
194
wild-type
198
substrate cyclosarin, pH 8.0, temperature not specified in the publication
199
mutant F314A
2.4
-
O-cyclohexyl methylphosphonofluoridate (-)isomer as substrate, pH 7.2, 1 mM MnCl2
2.7
-
O-cyclohexyl methylphosphonofluoridate (-)isomer as substrate, pH 7.2, 1 mM MnCl2
200
mutant D232S
209
mutant Y144S
225
substrate diisopropyl fluorophosphate, pH 8.0, temperature not specified in the publication
29.2
-
O-cyclohexyl methylphosphonofluoridate (+)isomer as substrate, pH 7.2, 1 mM MnCl2
31
mutant F173A
382.39
purified recombinant engineered His-tagged InaV-N-DFPase enzyme, substrate paraoxon, pH 7.2, 30°C
4
mutant N175D
63
mutant F173S
65.3
-
O-cyclohexyl methylphosphonofluoridate (+)isomer as substrate, pH 7.2, 1 mM MnCl2
7
mutant N120D
78
mutant T195A
82
substrate soman, pH 8.0, temperature not specified in the publication
84
mutant H287Y
90
mutant H287Q
92
mutant F173Y
97
mutant Q304F
additional information