3.1.8.2: diisopropyl-fluorophosphatase

This is an abbreviated version!
For detailed information about diisopropyl-fluorophosphatase, go to the full flat file.

Word Map on EC 3.1.8.2

3.1.8.2

dfpase

organophosphorus

dfp

degradation

squid

paraoxonase

sarin

phosphotriesterases

paraoxon

environmental protection

loligo

opaas

arylesterase

bioscavenger

vx

methylphosphonofluoridate

medicine

cyclosarin

six-bladed

carbanilate

udp-glucosyltransferase

diisopropylphosphorofluoridate

2.8.2.1

arylsulfotransferase

udp-glucuronyltransferase

2.3.1.5

analysis

Reaction

Synonyms

DFPase, di-isopropylphosphorofluoridate fluorohydrolase, dialkylfluorophosphatase, diisopropyl fluorophosphatase, diisopropyl phosphorofluoridate hydrolase, diisopropylfluorophosphatase, diisopropylfluorophosphonate dehalogenase, diisopropylphosphofluoridase, EC 3.8.2.1, h-PON1, isopropylphosphorofluoridase, More, OP-hydrolyzing enzyme, OPA anhydrase, OPA anhydrolase, OPAA, OPAA-2, opd, OPH, organophosphate acid anhydrase, organophosphate anhydrolase/prolidase, organophosphorus acid anhydrolase, organophosphorus hydrolase, organophosphorus-hydrolyzing enzyme, paraoxonase 1, phosphotriesterase, PON1, PTES5, RC, regucalcin, senescence marker protein-30, SMP30, somanase, tabunase

ECTree

3 Hydrolases

3.1 Acting on ester bonds

3.1.8 Phosphoric-triester hydrolases

3.1.8.2 diisopropyl-fluorophosphatase

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Results	in table
22	Activating Compound
13	AA Sequence
29	Application
1	CAS Registry Number
20	Cloned(Commentary)
14	Crystallization (Commentary)
1	Expression
21	General Information
4	General Stability
36	Inhibitors
59	kcat/KM [mM/s]
5	Ki Value [mM]
76	KM Value [mM]
8	Localization
54	Metals/Ions
15	Molecular Weight [Da]
25	Natural Substrates/ Products (Substrates)
86	Organism
49	PDB ID
11	pH Optimum
3	pH Range
1	pH Stability
1	pI Value
2	Posttranslational Modification
86	Protein Variants
23	Purification (Commentary)
16	Reaction
1	Reaction Type
74	Reference
1	Renatured (Commentary)
42	Source Tissue
54	Specific Activity [micromol/min/mg]
4	Storage Stability
247	Substrates and Products (Substrate)
20	Subunits
98	Synonyms
1	Systematic Name
10	Temperature Optimum [°C]
1	Temperature Range [°C]
1	Temperature Stability [°C]
54	Turnover Number [1/s]

Purification

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Purification on EC 3.1.8.2 - diisopropyl-fluorophosphatase

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8 entries

Ni-NTA column chromatography and Q Sepharose column chromatography

Loligo vulgaris

690417

Ni-NTA column chromatography and Q-Sepharose column chromatography

2 entries

partial

2 entries

partial, 3 enzymes of different MW

Rangia cuneata

646551

recombinant enzyme, production of large amounts

Loligo vulgaris

646561

recombinant His-tagged wild-type and engineered enzymes from Escherichia coli strain BL21 (IDE3) pLysS

Loligo vulgaris

Q7SIG4

749614

recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography

Sphingobium fuliginis

P0A433

751964

recombinant human paraoxonase 1 fused to human immunoglobulin Fc domain from Drosophila melanogaster S2 cells by protein A affinity chromatography and dialysis. Recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis

Homo sapiens

P27169

752102

recombinant wild-type and mutant enzymes refolded from Escherichia coli strain BL21(DE3) inclusion bodies by ion exchange chromatography

Homo sapiens

P27169

749628

SMP30 49fold from liver by anion exchange and hydrophobic interaction chromatography, and gel filtration

Rattus norvegicus

Q03336

664937