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3.1.8.2: diisopropyl-fluorophosphatase

This is an abbreviated version!
For detailed information about diisopropyl-fluorophosphatase, go to the full flat file.

Word Map on EC 3.1.8.2

Reaction

diisopropyl fluorophosphate
+
H2O
=
diisopropyl phosphate
 +
fluoride

Synonyms

DFPase, di-isopropylphosphorofluoridate fluorohydrolase, dialkylfluorophosphatase, diisopropyl fluorophosphatase, diisopropyl phosphorofluoridate hydrolase, diisopropylfluorophosphatase, diisopropylfluorophosphonate dehalogenase, diisopropylphosphofluoridase, EC 3.8.2.1, h-PON1, isopropylphosphorofluoridase, More, OP-hydrolyzing enzyme, OPA anhydrase, OPA anhydrolase, OPAA, OPAA-2, opd, OPH, organophosphate acid anhydrase, organophosphate anhydrolase/prolidase, organophosphorus acid anhydrolase, organophosphorus hydrolase, organophosphorus-hydrolyzing enzyme, paraoxonase 1, phosphotriesterase, PON1, PTES5, RC, regucalcin, senescence marker protein-30, SMP30, somanase, tabunase

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.8 Phosphoric-triester hydrolases
                3.1.8.2 diisopropyl-fluorophosphatase

Inhibitors

Inhibitors on EC 3.1.8.2 - diisopropyl-fluorophosphatase

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-hydroxyquinoline
a specific reversible competitive inhibitor of h-PON1 that is known to bind in the active site of the enzyme and inhibit the hydrolytic activities of the enzyme
Acetylcholine
-
high concentration
Butyrylcholine
-
high concentration
fluoride
product fluoride effectively inhibits organophosphorus hydrolase-catalyzed hydrolysis of diisopropylfluorophosphate (DFP) by a linear mixed inhibition, which is partially released by triethanolamine adding at initial or later reaction stage
Hg2+
-
strong
iodoacetamide
-
-
iodoacetic acid
Mipafox
N,N'-diisopropyldiamidofluorophosphate
i.e. mipafox or DDFP. the inhibitor's hydrolysis product, N,N'-diisopropyldiamidophosphate, is present in the cocrystal structure and bound by coordinating the binuclear metals and forming hydrogen bonds and nonpolar interactions with active site residues. An unusual common feature of the binding of the two ligands is the involvement of only one oxygen atom of the glycolate carboxylate and the product DDP tetrahedral phosphate in bridging the two Mn2+ ions, binding structure analysis, detailed overview
N-ethylmaleimide
O,O-dicyclopentylphosphoroamidate
crystallization data, phosphoryl oxygen of inhibitor is directly coordinated to the catalytic calcium ion
additional information
-