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3.1.8.2: diisopropyl-fluorophosphatase

This is an abbreviated version!
For detailed information about diisopropyl-fluorophosphatase, go to the full flat file.

Word Map on EC 3.1.8.2

Reaction

diisopropyl fluorophosphate
+
H2O
=
diisopropyl phosphate
 +
fluoride

Synonyms

DFPase, di-isopropylphosphorofluoridate fluorohydrolase, dialkylfluorophosphatase, diisopropyl fluorophosphatase, diisopropyl phosphorofluoridate hydrolase, diisopropylfluorophosphatase, diisopropylfluorophosphonate dehalogenase, diisopropylphosphofluoridase, EC 3.8.2.1, h-PON1, isopropylphosphorofluoridase, More, OP-hydrolyzing enzyme, OPA anhydrase, OPA anhydrolase, OPAA, OPAA-2, opd, OPH, organophosphate acid anhydrase, organophosphate anhydrolase/prolidase, organophosphorus acid anhydrolase, organophosphorus hydrolase, organophosphorus-hydrolyzing enzyme, paraoxonase 1, phosphotriesterase, PON1, PTES5, RC, regucalcin, senescence marker protein-30, SMP30, somanase, tabunase

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.8 Phosphoric-triester hydrolases
                3.1.8.2 diisopropyl-fluorophosphatase

Cloned

Cloned on EC 3.1.8.2 - diisopropyl-fluorophosphatase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli DH5alpha cells
-
expression in Escherichia coli
expression in Pichia pastoris
-
gene opd, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
gene opd, recombinant expression of wild-type and mutant MBP-tagged enzymes in Escherichia coli strain BL21(DE3)
gene PON1, recombinant expression of the human paraoxonase 1 in Drosophila melanogaster S2 stable cell line with induction by CuSO4 for 14 days. The recombinant human PON1 is fused with the human immunoglobulin Fc domain (PON1-hFc) to improve protein stability and purification efficiency, method optimization, overview. Recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) harboring the pGTf2 plasmid expressing GroEL-GroES and TF chaperones
gene PON1, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) in inclusion bodies, subcloning in Escherichia coli strain DH5alpha
phylogenetic analysis
recombinant expression of His-tagged wild-type and engineered enzymes in Escherichia coli strain BL21 (IDE3) pLysS