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3.1.6.2: steryl-sulfatase

This is an abbreviated version!
For detailed information about steryl-sulfatase, go to the full flat file.

Word Map on EC 3.1.6.2

Reaction

3beta-hydroxyandrost-5-en-17-one 3-sulfate
+
H2O
=
3beta-hydroxyandrost-5-en-17-one
+
sulfate

Synonyms

3-beta-hydroxysteroid sulfate sulfatase, arylsufatase, arylsulfatase C, ASC, AtsA, cholesterol sulfate sulfohydrolase, CHS-ase, dehydroepiandrosterone sulfatase, dehydroepiandrosterone sulfate sulfatase, DHEAS, E1-STS, ES, estrone sulfatase, More, neurosteroid sulfatase, NSS, oestrone sulfatase, phenolic steroid sulfatase, pregnenolone sulfatase, steroid 3-sulfatase, steroid sulfatase, steroid sulfate sulfohydrolase, steroid sulphatase, sterol sulfatase, sterolsulfate sulfohydrolase, Steryl-sulfate sulfohydrolase, STS, sulfatase, sterol

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.6 Sulfuric-ester hydrolases
                3.1.6.2 steryl-sulfatase

Subunits

Subunits on EC 3.1.6.2 - steryl-sulfatase

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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
coprecipitation of mutant enzyme with FLAG-tagged enzyme
heterotetramer
-
alpha2,beta2, 2 * 57000 + 2 * 38000, two 57 kDa catalytic and two 38 kDa regulatory subunits, SDS-PAGE
hexamer
monomer
-
1 * 78000, solubilized in Triton X-100
tetramer
-
4 * 72000, SDS-PAGE
trimer
-
alpha, 3 * 78000, SDS-PAGE,solubilized in Triton X-100
additional information
-
all oligomeric states between a monomer and a tetramer are possible, varying aggregation properties of enzyme under various buffer, pH, detergent and ionic strength conditions, gel filtration suggests ES to be predominantly a homotetramer, native PAGE analysis of solubilized single crystals of ES shows all possible oligomeric states between a monomer and a tetramer, but predominantly the monomeric and dimeric states