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3.1.6.2: steryl-sulfatase

This is an abbreviated version!
For detailed information about steryl-sulfatase, go to the full flat file.

Word Map on EC 3.1.6.2

Reaction

3beta-hydroxyandrost-5-en-17-one 3-sulfate
+
H2O
=
3beta-hydroxyandrost-5-en-17-one
 +
sulfate

Synonyms

3-beta-hydroxysteroid sulfate sulfatase, arylsufatase, arylsulfatase C, ASC, AtsA, cholesterol sulfate sulfohydrolase, CHS-ase, dehydroepiandrosterone sulfatase, dehydroepiandrosterone sulfate sulfatase, DHEAS, E1-STS, ES, estrone sulfatase, More, neurosteroid sulfatase, NSS, oestrone sulfatase, phenolic steroid sulfatase, pregnenolone sulfatase, steroid 3-sulfatase, steroid sulfatase, steroid sulfate sulfohydrolase, steroid sulphatase, sterol sulfatase, sterolsulfate sulfohydrolase, Steryl-sulfate sulfohydrolase, STS, sulfatase, sterol

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.6 Sulfuric-ester hydrolases
                3.1.6.2 steryl-sulfatase

Cloned

Cloned on EC 3.1.6.2 - steryl-sulfatase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
2.4 kb STS cDNA, expression in MCF-7 and COS-1 cells
-
expression in COS-7 cell
expression in Escherichia coli
expression in Escherichia coli and CHO cells
-
expression in monkey kidney COS-1 cells
-
expression in mouse A9 cells
-
His6-tagged wild-type STS cDNA subcloned into pCEP4, transfected into 293-EBNA cells, cell line with stable overexpression of STS-His6 selected for production of tagged wildtype STS, subsequent specific antibody production, mutated STS is transfected into human MCF-7 cells and monkey COS-1 cells to test for STS activity by immunoblotting
overexpression of STS gene in human breast cancer MCF-7 cells, i.e. MCS-2 cells, transplanted in female nude mice
-
partial sequence of porcine StS, subcloned into pGEM T vector, amplified in Escherichia coli