3.1.4.55: phosphoribosyl 1,2-cyclic phosphate phosphodiesterase
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For detailed information about phosphoribosyl 1,2-cyclic phosphate phosphodiesterase, go to the full flat file.
Reaction
Synonyms
phnP
ECTree
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Engineering
Engineering on EC 3.1.4.55 - phosphoribosyl 1,2-cyclic phosphate phosphodiesterase
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D80A
H200A
10% of wild-type activity, 6.33fold decrease in kcat/KM with substituted methyl phenylphosphate diesters with leaving group pKa values ranging from 4 to 8.4. With bis(pnitrophenyl)phosphate as a substrate, there is a 10fold decrease in kcat/KM, primarily the result of a large increase in KM. The nickel ion-activated H200A mutant displays a bell-shaped pH dependence for kcat/KM with pKa values comparable to those of the wild-type enzyme