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3.1.4.55: phosphoribosyl 1,2-cyclic phosphate phosphodiesterase

This is an abbreviated version!
For detailed information about phosphoribosyl 1,2-cyclic phosphate phosphodiesterase, go to the full flat file.

Reaction

5-phospho-alpha-D-ribose 1,2-cyclic phosphate
+
H2O
=
alpha-D-ribose 1,5-bisphosphate

Synonyms

phnP

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.4 Phosphoric-diester hydrolases
                3.1.4.55 phosphoribosyl 1,2-cyclic phosphate phosphodiesterase

Engineering

Engineering on EC 3.1.4.55 - phosphoribosyl 1,2-cyclic phosphate phosphodiesterase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D164A
0.07% of wild-type activity
D187A
83% of wild-type activity
D54A
0.09% of wild-type activity
H200A
10% of wild-type activity, 6.33fold decrease in kcat/KM with substituted methyl phenylphosphate diesters with leaving group pKa values ranging from 4 to 8.4. With bis(pnitrophenyl)phosphate as a substrate, there is a 10fold decrease in kcat/KM, primarily the result of a large increase in KM. The nickel ion-activated H200A mutant displays a bell-shaped pH dependence for kcat/KM with pKa values comparable to those of the wild-type enzyme
H222A
14% of wild-type activity
H78A
0.3% of wild-type activity
T75A
3% of wild-type activity