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3.1.4.53: 3',5'-cyclic-AMP phosphodiesterase

This is an abbreviated version!
For detailed information about 3',5'-cyclic-AMP phosphodiesterase, go to the full flat file.

Word Map on EC 3.1.4.53

Reaction

adenosine 3',5'-cyclic phosphate
+
H2O
=
AMP

Synonyms

3',5'-cAMP phosphodiesterase, 3',5'-cyclic AMP phosphodiesterase, adenosine 3',5'-cyclic monophosphate PDE, adenosine 3',5'-cyclicmonophosphate-specific PDE, cAMP phosphodiesterase, cAMP phosphodiesterase-4, cAMP-PDE, cAMP-phosphodiesterase, cAMP-phosphodiesterase 1C, cAMP-phosphodiesterase 4D7, cAMP-specific 3',5'-cyclic phosphodiesterase 4D, cAMP-specific cyclic nucleotide phosphodiesterase, cAMP-specific PDE, cAMP-specific PDE4A5, cAMP-specific PDE4D2, cAMP-specific phosphodiesterase, cAMP-specific phosphodiesterase 4D, cAMP-specific phosphodiesterase 4D11, cAMP-specific phosphodiesterase-4D5, cAMPspecific PDE, class I phosphodiesterase PDEB1, class I phosphodiesterase PDEB2, CpdA, CPDS, cyclic AMP phosphodiesterase type 4, cyclic AMP phosphodiesterase-4, cyclic AMP-specific phosphodiesterase, cyclic nucleotide phosphodiesterase, cyclic nucleotide phosphodiesterase 4, cyclic nucleotide phosphodiesterase 4D, cyclic nucleotide phosphodiesterase-8A, DdPDE4, high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A, hPDE4A, hPDE4B, HSPDE4A4B, LmjPDEB1, LmjPDEB2, PDE, PDE IVB, PDE-46, PDE-4D3, PDE10A, PDE1C, PDE2, PDE3, PDE4, PDE4A, PDE4A cAMP-specific phosphodiesterase splice variant RD1, PDE4A1, PDE4A10, PDE4A4, PDE4A5, PDE4A8, PDE4B, PDE4B1, PDE4B2, PDE4B3, PDE4B4, PDE4B5, PDE4C, PDE4C2, PDE4D, PDE4D1, PDE4D11, PDE4D3, PDE4D5, PDE4D7, PDE5, PDE7, PDE7A, PDE7A1, PDE7A2, PDE7B, PDE8, PDE8A, PDE8A1, PDE8B, PdeA, PdeB, PDEB1, PdeH, PdeL, phosphodiesterase 4, phosphodiesterase 4 isoform A8, phosphodiesterase 4B, phosphodiesterase 4D, phosphodiesterase 5, phosphodiesterase 7, phosphodiesterase 7B, phosphodiesterase type 4, phosphodiesterase type 5, phosphodiesterase-4, phosphodiesterase-4A5, phosphodiesterase-4B, RegA, RNPDE4A1A, TbPDE1, TbPDE2B, TbrPDEB1, TbrPDEB2, TcPDE1, TcPDE4, TcrPDEA1, TcrPDEB1

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.4 Phosphoric-diester hydrolases
                3.1.4.53 3',5'-cyclic-AMP phosphodiesterase

Cloned

Cloned on EC 3.1.4.53 - 3',5'-cyclic-AMP phosphodiesterase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
baculovirus-expressed PDE4 in Sf9 cells
-
baculovirus-expressed PDE7 in Sf9 cells
-
cells of two human follicular thyroid carcinoma cell lines (FTC133, FTC236) are stably transfected with a cDNA encoding the PDE4A cAMP-specific phosphodiesterase (PDE) splice variant RD1 (RNPDE4A1A) so as to generate the cloned cell lines, FTC133A and FTC236A
cloned and expressed in Sf9 cells with recombinant baculovirus infection
-
Epac1-camps-PDE4A1 fusion protein is expressed in HEK-293 cells
expressed in COS-1 cells
-
expressed in COS1 cells
-
expressed in Escherichia coli
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli M15(pREP4) cells
expressed in Escherichia coli strain BL21
expressed in HEK-293 cells
-
expressed in Neuro-2A and NIH-3T3 cells
expressed in Rattus norvegicus INS-1 832/13 cell line
-
expressed in Schizosaccharomyces pombe
-
expressed in transfected COS7 cells. The human PDE4A species, h6.1 (HSPDE4A4C), which lacks the N-terminal extension of PDE-46, is an entirely soluble species when expressed in COS7 cells
-
expression in baculovirus transfected Sf9 cells
expression in COS-7 cell
expression in COS1 cells
expression in COS7 cells
-
expression in Escherichia coli
expression in Escherichia coli as N-terminal glutathione S-transferase-fusion protein
expression in Saccharomyces cerevisiae
expression in Sf9 insect cells
expression in Spodoptera frugiperda
-
expression of PDE4B4 cDNA in COS7 cells. Treatment of COS7 cells with forskolin, to elevate cAMP levels, produced activation of PDE4B4, which is associated with the phosphorylation of PDE4B4 on Ser56 within UCR1
expression of TcrPDEB1 catalytic domain in Escherichia coli
expression of the catalytic domain in Escherichia coli
-
full coding sequence of the phosphodiesterase PDE4D1 is inserted in the bacterial expression vector pGEX-KG. N- and C-terminal truncations are also placed in the same vector, allowing the expression and purification of glutathione S-transferase (GST)-PDE fusion proteins using glutathione-Sepharose
-
gene cpdA, DNA and mino acid sequenc determination and analysis, expression of His-tagged enzyme in Escherichia coli strain M15(pREP4)
PDE7B protein is expressed in a baculovirus expression system
recombinant PDE7B expressed in transfected COS-7 cells
-
TbPDE1 is expressed in Saccharomyces cerevisiae at levels that are sufficient to produce a clear phenotype (heat-shock resistance, growth as a smooth suspension), but that are too low to be detectable in PDE assay of cell lysates. The N-terminally truncated construct pET-PDE1-(Arg189-Thr620) is expressed in Escherichia coli as an active enzyme
-
the catalytic domain of human PDE4D is cloned from HL-60 cells and expressed in Escherichia coli JM109 cells
-
the DdPDE4 catalytic domain (amino acids 264-771) is expressed in vegetative AX2 cells
transient expression of the engineered human PDE4A10 open reading frame in COS7 cells allows detection of a 121000 Da protein in both soluble and particulate fractions. PDE4A10 is localized primarily to the perinuclear region of COS7 cells
-
When expressed in COS-7 cells, PDE4A8 localizes predominantly in the cytosol, but 20% of the enzyme was associated with membrane fractions