3.1.30.2: Serratia marcescens nuclease

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For detailed information about Serratia marcescens nuclease, go to the full flat file.

Reaction

endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products =

Synonyms

barley nuclease, BFN1, BMN, CAD, caspase-activated DNase, DFF, DFF40/CAD endonuclease, DNA fragmentation factor, EC 3.1.4.9, ENDO1, ENDO2, ENDO4, ENDO5, endonuclease, endonuclease (Serratia marcescens), endonuclease 1, endonuclease 2, endonuclease 4, endonuclease 5, Kamchatka crab duplex-specific nuclease, More, mung bean nuclease, NUC49, nucA, NucANLS, nuclease A, nuclease I, nuclease, nucleate endo-, nucleate endonuclease, Par_DSN, plant nuclease I, plant S1-like nuclease, plant type I nuclease, rNUC49, S1-like nuclease, Serratia marcescens endonuclease, Serratia marcescens nuclease, Sm2, Sma, Sma nuc, Sma nuc endonuclease, SMnase

ECTree

     3 Hydrolases

         3.1 Acting on ester bonds

             3.1.30 Endoribonucleases that are active with either ribo- or deoxyribonucleic acids and produce 5'-phosphomonoesters

                3.1.30.2 Serratia marcescens nuclease

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Results	in table
3	Activating Compound
39	AA Sequence
1	Application
1	CAS Registry Number
14	Cloned(Commentary)
3	Crystallization (Commentary)
2473	Disease/ Diagnostics
1	Expression
14	General Information
1	General Stability
68	Inhibitors
6	Ki Value [mM]
8	KM Value [mM]
11	Localization
35	Metals/Ions
15	Molecular Weight [Da]
22	Natural Substrates/ Products (Substrates)
49	Organism
10	PDB ID
20	pH Optimum
8	pH Range
3	pH Stability
1	pI Value
1	Posttranslational Modification
15	Protein Variants
21	Purification (Commentary)
6	Reaction
4	Reaction Type
45	Reference
2	Renatured (Commentary)
29	Source Tissue
28	Specific Activity [micromol/min/mg]
2	Storage Stability
116	Substrates and Products (Substrate)
9	Subunits
62	Synonyms
9	Temperature Optimum [°C]
2	Temperature Range [°C]
8	Temperature Stability [°C]
3	Turnover Number [1/s]

Expression

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Expression on EC 3.1.30.2 - Serratia marcescens nuclease

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EXPRESSION

ORGANISM

UNIPROT

LITERATURE

both NucA with and without a nuclear localization signal prove to be effective in killing porcine fibroblasts compared to controls, gene expression analysis of surviving colonies indicates that survival is related to low or absent expression of the toxic genes

Serratia marcescens

P13717

701699